Devices, methods and systems for target detection

ABSTRACT

Polymer arrays suitable to perform quantitative and qualitative detection as well as sorting of a target molecules and related devices methods and systems.

CROSS REFERENCE TO RELATED APPLICATIONS

This application claims priority to U.S. Provisional Application No.61/302,535 entitled “Using Phylogenetic Probes For Quantification OfStable Isotope Labeling And Microbial Community Analysis” filed on Feb.8, 2010, docket IB-2774P1 and with U.S. Provisional Application No.61/302,827 entitled “Chip-SIP: Quantification of Nucleic Acid StableIsotope Labeling with Biopolymer Microarrays and Secondary IonizationMass Spectrometry (SIMS)” filed on Feb. 9, 2010, with docket numberIL-12105, each of which is incorporated herein by reference in itsentirety. The present application may also be related to U.S.application Ser. No. 12/366,476 entitled “ ”Functionalized platform forarrays configured for optical detection of targets and related arrays,methods and systems” filed on Feb. 5, 2009 with docket IL-11703, and toU.S. application Ser. No. ______, entitled “Using Phylogenetic ProbesFor Quantification Of Stable Isotope Labeling And Microbial CommunityAnalysis” filed on Feb. 8, 2011 with docket IB-2774, each of which isherein also incorporated by reference in its entirety.

STATEMENT OF GOVERNMENT INTEREST

The United States Government has rights in this invention pursuant toContract No. DE-AC52-07NA27344 between the United States Department ofEnergy and Lawrence Livermore National Security, LLC for the operationof Lawrence Livermore National Laboratory

FIELD

The present disclosure relates to devices methods and systems for targetdetection. In particular, the present disclosure relates to devicesmethods and systems for detection of targets on a polymer array.

BACKGROUND

The application of molecular techniques has rapidly advanced thedetection and identification of targets in sample where targets ofvarious chemical natures are included. Several techniques are availablethat allow detection of target molecules such as polymers, and inparticular biopolymers, for various purposes, including, for example,identification of microorganisms and microbial systems.

However, reproducibility and/or quantification of targets can still bechallenging in particular when detection is performed using a polymerarray. Chemical similarities between the target molecules can interferewith the ability to accurately detect multiple targets. In certain casesability to predict the extent of hybridization and sensitivity of somerelated reporting techniques can make detection of specific moleculesand related quantitation quite challenging.

SUMMARY

Provided herein are devices, methods and systems configured for targetdetection through Secondary Ion Mass Spectrometry (SIMS), which, inseveral embodiments, allow quantitative and/or sensitive detection oftargets bound to a polymer array. In particular, in several embodiments,devices methods and systems herein described allow quantitative and/orsensitive detection of target polymers presenting SIMS detectable labelsfollowing binding of the target polymers with the polymer array.

According to a first aspect a method for quantitative detection of atarget is described. The method comprises, labeling a target with a SIMSdetectable label, which can in particular be formed by stable isotopeprobes, to provide a SIMS labeled target, the SIMS labeled targetcapable of binding a polymer of a polymer array herein described. Themethod further comprises contacting the SIMS labeled target with thepolymer array for a time and under conditions that allow binding of theSIMS labeled target molecule to the polymer array. The method alsocomprises performing SIMS detection of the polymer array following thecontacting to detect the SIMS labeled target bound to the polymer array.For the polymer array, the platform comprises a substrate coated with anelectrically conductive layer and the polymer is attached to theplatform through a functional linker molecule attached to theelectrically conductive layer.

According to a second aspect a method to detect a target in a sample isdescribed: The method comprises exposing the sample to a labeldetectable by Secondary Ion Mass Spectrometry (SIMS label) for a timeand under conditions that allow binding of the SIMS label with thetarget. The method further comprises contacting a polymer array with thesample to allow binding of the labeled target to the polymer array. Themethod also comprises performing Secondary Ion Mass Spectrometry on thepolymer array following the contacting in order to detect the SIMSlabeled target. In the polymer array, the platform comprises a substratecoated with an electrically conductive layer and the polymer is attachedto the platform through a functional linker molecule attached to theelectrically conductive layer.

According to a third aspect, a system for detection of a target isdescribed, that comprises a polymer array herein described, and a SIMSdetectable label. In some embodiments, the system can further includeSIMS detecting elements, such as suitable pieces of equipment to performdetection of a target comprising the SIMS detectable label.

According to a fourth aspect, a functionalized platform is described,that comprises a substrate having an electrically conductive surface,the electrically conductive surface attaching a functionalized linkermolecule comprising an organosilane presenting an organosilanefunctional group. The functionalized platform is also configured to beassociated, during operation, with a polymer array, through attachmentof the polymers of the polymer array with the functionalized linkermolecule, and the polymer array is configured for SIMS detection of atarget attached to a polymer on the polymer array, through a SIMSdetectable label attached to the target.

According to a fifth aspect, a polymer array is described that isconfigured to allow SIMS detection of a target attached to the polymerthrough a SIMS detectable label attached to the target. The polymerarray comprises a polymer attached to a functionalized platformdescribed herein wherein the polymer is attached to the functionalizedlinker molecules of the platform.

According to a sixth aspect, a bio-chip is described that comprises apolymer array herein described.

The platforms, arrays, methods and systems described allow in severalembodiments quantitative detection of targets such as a polymers and inparticular biopolymer comprising nucleic acids, polypeptides andadditional polymers identifiable by a skilled person.

The platforms, arrays, methods and systems described herein can be usedin connection with applications wherein quantitative detection sortingand/or analysis of targets of interest and in particular nucleic acidmolecules through an array is desired, including but not limited tomedical application, biological analysis and diagnostics including butnot limited to clinical applications.

The details of one or more embodiments of the disclosure are set forthin the accompanying drawings and the description below. Other features,objects, and advantages will be apparent from the description anddrawings, and from the claims.

BRIEF DESCRIPTION OF THE DRAWINGS

The accompanying drawings, which are incorporated into and constitute apart of this specification, illustrate one or more embodiments of thepresent disclosure and, together with the detailed description and theexamples, serve to explain the principles and implementations of thedisclosure.

FIG. 1 shows a schematic illustration of the basic coupling chemistrybetween phosphonate (phosphonic acid) and a metal oxide coated surfaceto generate a functionalized platform according to an embodiment of thepresent disclosure.

FIG. 2A shows oligonucleotides spotted onto a glass surface coated withthe industry standard triethoxy silane.

FIG. 2B shows an illustration of oligonucleotides spotted on a glasssurface coated with an alkyl phosphonate compound terminated with analkyl phosphonate surface. The illustration is comparative to thecorresponding oligonucleotide probes of FIG. 2A.

FIG. 2C shows a diagram illustrating the signal to noise ratio forfluorescent signal of the array of FIG. 2A (silane linker upper trace)and the array of FIG. 2B (ITO lower trace).

FIG. 3 shows a chart illustrating data from an indium tin oxide(referred to as ITO) coated array slide analysis by NanoSIMS. Ion countsfrom the NanoSIMS analysis indicate the suitability of ITO surfaces forSIMS analysis, as the conductive, oxide coating allows for consistention sputtering with depth.

FIG. 4 shows an ITO coated microarray slide analyzed by NanoSIMS. Thesuitability and stability of ITO coated arrays for SIMS analysis aredemonstrated. NanoSIMS secondary electron (left), and ¹⁸O and ¹²C ionimages indicating no evidence of sample charging. Clockwise from topleft: secondary electron (SE) image, silicon ion image (28Si), oxygenion image (16O), carbon ion image (12C).

FIG. 5A shows a post-hybridization fluorescence scan of an ITO alkylphosphonate array synthesized with multiple different Pseudomonasstutzeri probes and hybridized with RNA from ¹³C labeled P. stutzericells. The scale of fluorescence intensity moves from low to high, blackto white. Each square region is comprised of a 2×2 grid of separateprobe spots, all targeting the same DNA sequence.

FIG. 5B shows a montage of NanoSIMS ¹³C enrichment images collected froma nanoSIMS analysis of the same array shown in FIG. 5A. The scale ofenrichment moves from low to high, black-dark grey-medium grey-lightgray-white.

FIG. 5C shows a plot of the quantitative NanoSIMS data displayed in FIG.5B versus the fluorescence data shown in FIG. 5A. The correlationbetween the two is good, as evidenced by the R² value.

FIG. 6 shows fluorescence detection by microarray scanner (FIG. 6A) and¹³C enrichment by NanoSIMS (FIG. 6B) of RNA enriched with as little as0.5% following RNA hybridization to ITO microarray and then detectionvia NanoSIMS).

FIG. 7 shows a plot of quantitative NanoSIMS data versus hybridizationdata for an array hybridized with extracted RNA from two bacterialspecies (Vibrio cholera and Bacillus cereus) grown separately on twodifferent levels of ¹³C-glucose (FIG. 7A) and ¹⁵N-ammonium (FIG. 7B) astheir sole carbon and nitrogen source. RNA is from bacterial cultures ofVibrio cholerae (squares), and Bacillus cereus (triangles); background(diamond) values are displayed for reference. HCE=hybridizationcorrected enrichment, a metric which allows different populations of RNAmolecules to be compared with respect to their isotopic enrichment. Eachdata point represents a distinct probe specific for each bacterialspecies.

FIG. 8 illustrates detection by array fluorescence (FIG. 8A); and d¹⁵Nby NanoSIMS (FIG. 8B) of Pseudomonas stutzeri grown on 25% ¹⁵N ammonium,and Bacillus cereus grown on natural abundance ammonium; with RNAextracted, mixed in equal concentrations, hybridized on ITO-coatedarray.

FIG. 9 shows diagrams illustrating the results of experiments withsimple two-member communities performed with devices, methods andsystems herein described. The two member communities comprisePseudomonas stutzeri grown on 100% ¹³C glucose, and Vibrio cholera grownon 20% ¹³C glucose. In FIG. 9A, each data point represents a NanoSIMSanalysis of a single array probe spot (plotted against arrayfluorescence). In FIG. 9B a one-way ANOVA test, indicating astatistically significant difference between the two RNA populations(p<0.0001) is shown.

FIG. 10A shows an array designed to target marine microorganismsdesigned using ARB software; each row on the array represents a seriesof probes designed to hybridize to a different taxon (microbialspecies), as indicated.

FIG. 10B shows a schematic illustration of an analysis performed withdevices, methods and systems herein described. It quantitativelyillustrates the flow of three organic substrates to different bacterialtaxa in an estuary, identifying substrate specialists and generalists;the thicknesses of the lines are proportional to the substrateincorporation rates.

DETAILED DESCRIPTION

Devices, arrays methods and systems described herein are also indicatedas “Chip-SIP” that in several embodiments allow detection of a target ona polymer array through Secondary Ion Mass Spectrometry.

The term “detect” or “detection” as used herein indicates thedetermination of the existence, presence or fact of a target or signalin a limited portion of space, including but not limited to a sample, areaction mixture, a molecular complex and a substrate including aplatform and an array. A detection is “quantitative” when it refers,relates to, or involves the measurement of quantity or amount of thetarget or signal (also referred as quantitation), which includes but isnot limited to any analysis designed to determine the amounts orproportions of the target or signal. A detection is “qualitative” whenit refers, relates to, or involves identification of a quality or kindof the target or signal in terms of relative abundance to another targetor signal, which is not quantified. In several embodiments, the Chip-SIPdevices, methods and systems allows quantitative detection of single ormultiple targets.

The term “target” as used herein indicates an analyte of interest. Theterm “analyte” refers to a substance, compound or component whosepresence or absence in a sample has to be detected. Analytes include butare not limited to biomolecules and in particular biomarkers. The term“biomolecule” as used herein indicates a substance compound or componentassociated with a biological environment, especially the nucleic acidsDNA and RNA. The term “biomarker” indicates a biomolecule that isassociated with a specific state of a biological environment includingbut not limited to a phase of cellular cycle, health and disease state.The presence, absence, reduction, upregulation of the biomarker isassociated with and is indicative of a particular state. Biomoleculesthat are detectable through Chip-SIP include in particular biopolymers,which in certain embodiments can also be used as biomarkers.

According to various embodiments the Chip-SIP herein described,detection of a target can be performed through Secondary Ion MassSpectrometry analysis of a polymer array presenting a target, typicallyformed by one or more biopolymers.

The term “polymer array” as used herein indicates a regular and imposinggrouping or arrangement of polymer molecules immobilized on anappropriate or compatible substrate in an ordered manner, herein alsoindicated as probe polymers. More particularly, the term polymer arrayindicates an ordered grouping of probe polymers arranged so to allow,under appropriate conditions, specific binding of a target to at leastone of the polymer composing the polymer array and subsequent detectionof the target bound to the polymer.

In Chip-SIP detection, polymer arrays are attached on a functionalizedplatform through linkage with functional linker molecules attached on anelectrically conductive layer and presenting functional groups forbinding with probe polymers.

The term “platform” as used herein indicates a physical and usually flatstructure suitable for carrying a polymer array. A platform typicallycomprises a substrate functionalized to be capable of reacting with apolymer of the polymer array and the polymer array.

The term “substrate” as used herein indicates a base material on whichprocessing can be conducted to modify the chemical nature of at leastone surface of the base material. Exemplary chemical modificationsinclude functionalization and/or depositing on the modified surface alayer of a second material chemically different from the base material.Exemplary substrates in the sense of the present disclosure include butare not limited to glass, such as silica-based glass, plastics, such ascyclo-olefin copolymer, carbonates and the like, and silicon materials,such as the ones used in the electronic industry. The substrate can betwo dimensional such as a typical glass microscope slide of standarddimension, i.e. 25 mm×75 mm.

In platform described herein a substrate is coated with a functionalizedelectrically conductive layer that can be formed by a metal oxide layer.The term “layer” as used herein indicates a single thickness of materialcovering a surface. Accordingly, a metal oxide layer is a thickness of ametal oxide compound covering a substrate surface of the substrate ofthe platform or a portion thereof.

The term “metal oxide” as used herein indicates a compound including atleast one oxygen atom bound to a metal atom. Exemplary metal oxidesinclude in particular amphoteric metal oxide such as aluminum oxide andother metal oxides wherein the metal element is in a +3 oxidation state,tin oxide other metal oxides wherein the metal element is in a +4oxidation state or mixture thereof. In an embodiment, the metal oxidecomprises Indium Tin Oxide, a solid solution of indium (III) oxide(In₂O₃) and tin oxide (SnO₂), typically 90% In₂O₃, 10% SnO₂ by weight,which is a particularly suitable electrically conductive material.

In platform herein described, a metal oxide thickness can be applied tothe substrate by deposition of the metal oxide performed by techniquesidentifiable by a skilled person. In particular, in several embodimentsherein disclosed, the surface of a substrate is coated by the metaloxide, wherein the term “coat” and “coating” indicates a covering of themetal oxide applied to the surface using techniques known in the art.Exemplary techniques suitable to apply a coating to a substrate includechemical vapor deposition, conversion coating, plating and othertechniques identifiable by a skilled person. In case of ITO thin filmsof indium tin oxide coating procedures can be performed by electron beamevaporation, physical vapor deposition, or a range of sputter depositiontechniques. Concentration of charge carriers during deposition isselected in view of the desired electrical conductivity since a highconcentration will increase the material's conductivity, but decreaseits transparency.

In platforms and the microarray herein described, the metal oxide isfunctionalized to allow attachment of a polymer array. The terms“functionalize” and “functionalization” as used herein, indicates theappropriate chemical modifications of a molecular structure (including asubstrate or a compound) resulting in attachment of a functional groupto the molecular structure. The term “functional group” as used hereinindicates specific groups of atoms within a molecular structure that areresponsible for the characteristic chemical reactions of that structure.Exemplary functional groups include, hydrocarbons, groups containinghalogen, groups containing oxygen, groups containing nitrogen and groupscontaining phosphorus and sulfur all identifiable by a skilled person.The term “attach” or “attached” as used herein, refers to connecting oruniting by a bond, link, force or tie in order to keep two or morecomponents together, which encompasses either direct or indirectattachment such that for example where a first compound is directlybound to a second compound or material, and the embodiments wherein oneor more intermediate compounds, and in particular molecules, aredisposed between the first compound and the second compound or material.

In platforms herein described the electrically conductive layer isfunctionalized to attach an alkyl phosphonate compound that presents analkyl phosphonate functional group and/or with organosilanes thatpresents an organosilane functional group. The term “present” as usedherein with reference to a compound or functional group indicatesattachment performed to maintain the chemical reactivity of the compoundor functional group as attached. Accordingly, a functional grouppresented on a surface is able to perform under the appropriateconditions the one or more chemical reactions that chemicallycharacterize the functional group.

In particular, in some embodiments, the metal oxide layer is treatedwith a solution of a functionalized alkyl phosphonate compound. In thoseembodiments, the phosphonates form an ordered monolayer on the metaloxide surface and are covalently linked to the metal oxide via formationof stable metal-phosphodiester bonds as has been well-established inpublished scientific literature. In some embodiments, the metal oxide isfunctionalized with an organosilane, e.g. triethoxyaminoproply silane orother organosilane identifiable by a skilled person. Thealkylphosphonate functional group and/or organosilane functional groupsare used to attach probe polymers of a polymer array.

The term “polymer” as used herein indicates a large molecule(macromolecule) composed of repeating structural units typicallyconnected by covalent chemical bonds. Polymers constitute a large classof natural and synthetic materials with a variety of properties andpurposes and include bio-polymers which are the typical polymercomponent of polymer arrays as identified herewith. Biopolymers comprisepolysaccharides polymers made up of many monosaccharides joined togetherby glycosidic bonds, polynucleotide and polypeptides that are originallyproduced by living organisms including viruses.

The term “polynucleotide” as used herein indicates an organic polymercomposed of two or more monomers including nucleotides, nucleosides oranalogs thereof. The term “nucleotide” refers to any of severalcompounds that consist of a ribose or deoxyribose sugar joined to apurine or pyrimidine base and to a phosphate group and that is the basicstructural unit of nucleic acids. The term “nucleoside” refers to acompound (such as guanosine or adenosine) that consists of a purine orpyrimidine base combined with deoxyribose or ribose and is foundespecially in nucleic acids. The term “nucleotide analog” or “nucleosideanalog” refers respectively to a nucleotide or nucleoside in which oneor more individual atoms have been replaced with a different atom or awith a different functional group. Accordingly, the term polynucleotideincludes nucleic acids of any length including DNA, RNA, DNA or RNAanalogs and fragments thereof. A polynucleotide of three or morenucleotides is also called a nucleotidic oligomers or oligonucleotide.Exemplary polynucleotides composing arrays herein disclosed are DNAmolecules, and in particular DNA oligomers, peptide nucleic acids(PNAs), locked nucleic acid polymers (LNAs) and the like.

The term “peptide nucleic acid” indicates an artificially synthesizedpolymer similar to DNA or RNA and is used in biological research andmedical treatments. PNA is not known to occur naturally. In particular,while DNA and RNA have a deoxyribose and ribose sugar backbone,respectively, whereas PNA's backbone is composed of repeatingN-(2-aminoethyl)-glycine units linked by peptide bonds. The variouspurine and pyrimidine bases are linked to the backbone by methylenecarbonyl bonds. PNAs are depicted like peptides, with the N-terminus atthe first (left) position and the C-terminus at the right.

The term “locked nucleic acid”, often referred to as inaccessible RNA,indicates a modified RNA nucleotide. The ribose moiety of an LNAnucleotide is modified with an extra bridge connecting the 2′ and 4′carbons. The bridge “locks” the ribose in the 3′-endo structuralconformation, which is often found in the A-form of DNA or RNA. LNAnucleotides can be mixed with DNA or RNA bases in the oligonucleotidewhenever desired. Such oligomers are commercially available. The lockedribose conformation enhances base stacking and backbonepre-organization. This significantly increases the thermal stability(melting temperature) of oligonucleotides. LNA nucleotides are used toincrease the sensitivity and specificity of expression in DNAmicroarrays, FISH probes, real-time PCR probes and other molecularbiology techniques based on oligonucleotides. For the in situ detectionof miRNA the use of LNA is currently (2005) the only efficient method. Atriplet of LNA nucleotides surrounding a single-base mismatch sitemaximizes LNA probe specificity unless the probe contains the guaninebase of G-T mismatch.

The term “polypeptide” as used herein indicates an organic polymercomposed of two or more amino acid monomers and/or analogs thereof. Theterm “polypeptide” includes amino acid polymers of any length includingfull length proteins and peptides, as well as analogs and fragmentsthereof. A polypeptide of three or more amino acids is also called aprotein oligomer or oligopeptide. As used herein the term “amino acid”,“amino acidic monomer”, or “amino acid residue” refers to any of thetwenty naturally occurring amino acids including synthetic amino acidswith unnatural side chains and including both D and L optical isomers.The term “amino acid analog” refers to an amino acid in which one ormore individual atoms have been replaced, either with a different atom,isotope, or with a different functional group but is otherwise identicalto its natural amino acid analog.

The term “protein” as used herein indicates a polypeptide with aparticular secondary and tertiary structure that can participate in, butnot limited to, interactions with other biomolecules including otherproteins, DNA, RNA, lipids, metabolites, hormones, chemokines, and smallmolecules. Exemplary proteins composing arrays herein described areantibodies.

The term “antibody” as used herein refers to a protein that is producedby activated B cells after stimulation by an antigen and bindsspecifically to the antigen promoting an immune response in biologicalsystems and that typically consists of four subunits including two heavychains and two light chains. The term antibody includes natural andsynthetic antibodies, including but not limited to monoclonalantibodies, polyclonal antibodies or fragments thereof. Exemplaryantibodies include IgA, IgD, IgG1, IgG2, IgG3, IgM and the like.Exemplary fragments include Fab Fv, Fab′ F(ab′)2 and the like. Amonoclonal antibody is an antibody that specifically binds to and isthereby defined as complementary to a single particular spatial andpolar organization of another biomolecule which is termed an “epitope”.A polyclonal antibody refers to a mixture of monoclonal antibodies witheach monoclonal antibody binding to a different antigenic epitope.Antibodies can be prepared by techniques that are well known in the art,such as immunization of a host and collection of sera (polyclonal) or bypreparing continuous hybridoma cell lines and collecting the secretedprotein (monoclonal).

In polymer array herein described, any of the above polymers can besynthesized or added and in particular spotted on a coated substrateaccording to techniques identifiable by a skilled person.

Applicants have surprisingly found that probe polymer arrays onfunctionalized platforms herein described allow detection of properlylabeled target performed through SIMS.

The term “SIMS” or “Secondary Ion Mass Spectrometry” as used hereinindicates a technique typically used in materials science and surfacescience to analyze the composition of solid surfaces and thin films bysputtering the surface of the specimen with a focused primary ion beamand collecting and analyzing ejected secondary ions. These secondaryions are typically measured with a mass spectrometer or other SIMSdetecting elements to determine the elemental, isotopic, or molecularcomposition of the surface. In several applications, SIMS is one of themost sensitive surface analysis techniques able to detect elementspresent in the parts per billion range. Exemplary procedures anddetecting elements suitable for SIMS analysis are described for examplein the enclosed references (see e.g. ref (1) and ref (15)). A skilledperson will be able to identify additional instruments and proceduresthat are suitable for the implementation of Chip-SIP herein describedupon reading of the present disclosure.

A detection performed through SIMS, or “SIMS detection” is performedthrough measurement of a SIMS detectable signal typically issued by aSIMS label on a surface following sputtering of the surface with afocused primary ion beam. The terms “label” and “labeled molecule” asused herein refer to any elemental label capable of detection, which ingeneral comprise radioactive isotopes, stable isotopes, halogenatedoligonucleotide probes, metal ions, nanoparticles, and the like. As aconsequence the wording “labeling signal” indicates in general thesignal emitted from the label that allows detection of the label.

A “SIMS label” as used herein indicates a label capable of issuing aSIMS detectable signal on a surface following sputtering of the surfacewith a focused primary ion beam. A “SIMS detectable signal” indicates asignal that is detectable through the use of SIMS detecting elements,(e.g. a sector, quadrupole, and time-of-flight mass analyzer) A SIMSdetectable signal is typically in the form of characteristic secondaryions detectable through any appropriate SIMS detecting element as willbe understood by a skilled person. Exemplary SIMS labels comprise stableisotopes wherein the term “stable isotope” refers to a non-radioactiveisotopic form of an element, which can include, but is not limited to,¹³C or ¹⁵N, ¹⁸O, ¹⁹E, ¹²⁷I, ⁷⁹Br, or ¹⁹⁷Au.

Metal oxide layers and in particular layers formed by comprising IndiumTin Oxide (ITO), are particularly suitable for SIMS analysis because oftheir conductive properties and stability under reduced pressure.Presentation of probe polymers on such an electrically conducting layer,made possible by use of functionalized linker molecules such asphosphohonate or organosilane, in combination with use of SIMSdetectable label has enabled a detection of single and in particularmultiple targets that in some embodiments, is significantly moresensitive of corresponding approach of the art. Additional detailsconcerning procedures specific embodiments of platform presenting alkylphosphonate functional groups are described in US Pat. Application US2009-0203549 and International Application WO 2009/100201, each of whichis herein incorporated in its entirety.

In several embodiments of the Chip-SIP devices, methods and systems,detection of a SIMS signal issued by a SIMS labeled target bound on apolymer array herein described allows quantitative and/or qualitativedetection of target.

In particular in some embodiments a quantitative detection of a targetcan be performed by labeling the target with a SIMS detectable label toprovide a SIMS labeled target, the SIMS labeled target capable ofbinding a polymer of a polymer array herein described.

Suitable labeling procedures depend on the target and desired detection.For example nucleic acids can be labeled by incorporating ¹³C and/or ¹⁵Nin the nucleic acids during synthesis which can be performed within acell, or in vitro e.g. in a cell free system. Additional labeling can beperformed by attaching gold nanoparticles or halogen atoms (F, I, Br) toDNA or RNA. In an embodiment, the labeling can be performed by exposinga sample to a SIMS-label to allow binding of the SIMS label with atarget whose quantity or presence in the sample one wants to detect. Theterm “exposing” or “expose” or “to expose” as used herein refers to acontacting of the sample performed to allow the introduction ofSIMS-label (e.g. stable isotopes) to a sample, to allow attachment ofthe SIMS-label in the target if present in the sample. By way ofexample, in embodiments where detection of nucleic acids in microbes isdesired, a bacterial population can be grown on a substrate enrichedwith a SIMS label formed by stable isotopes. By way of example, bacteriacan be grown in a liquid media substance containing stable isotopeswherein the bacteria feed off the stable isotope-containing liquidmedia. Additional methods for enabling the attachment of a SIMS-labelonto a target in a sample are identifiable by a skilled person dependingon the specific target and label selected for the detection.

In an embodiment, the SIMS labeled target resulting from a labelingprocedure is then contacted with a polymer array for a time and underconditions to allow binding of the SIMS labeled target molecule to thepolymer array.

In some embodiments, the contacting is performed by isolating thelabeled target from a sample comprising the target (e.g. by extractionof nucleic acids from an organism) and then contacting the isolatedtarget with a polymer array herein described.

In particular, in embodiments, where the target are also biopolymers thecontacting can be performed by hybridization of the probe polymers withthe target polymer. The term “hybridize” or “hybridization” or“hybridized” as used herein refers to a process by which single strandsof nucleic acid sequences form double-helical segments via hydrogenbonding between complementary nucleotides covalently bonded to afunctionalized platform. Other forms of specific binding between probepolymers and target herein described will be identifiable by a skilledperson. Additional forms of contacting include protein-proteininteractions, antigen-antibody interaction, nucleic acid proteininteraction and additional interactions identifiable by a skilled personupon reading of the present disclosure.

In some embodiments, the contacting is performed with a polymer arraythat comprises an arrayed series of thousands of microscopic spots ofthe polymer of interest, called features, each containing a smallamount, (e.g. picomoles) of a specific probe polymer and in particular aprobe biopolymer, (for example a DNA polymer having a specificsequence). Exemplary biopolymers include, a short section of a gene orother DNA element that are used as stationary probes capable of bindingto added sample molecule (target) under conditions or varying bindingstringency. In an embodiment, arrays can include but are not limited to:features ranging in size from 25 square microns (μ²) to 250 squaremicrons (μ²) that are made by mechanically (robotically) or manuallyspotting a defined volume of polymer on the substrate surface. In anembodiment microarrays can include but are not limited to featuresranging in size from 5 square microns (μ²) to 250 square microns (μ²)that are prepared by de novo synthesis of a plurality of definedbiopolymer material, e.g. DNA probes; using established solid phasesynthetic chemistry. In some embodiments, probe polymers are used thecomprise oligonucleotides between 25 and 50 base pairs, although oneskilled in the art would recognize that oligonucleotides that are muchshorter than 25 base pairs, or significantly longer than 50 base pairscould be used. Probe arrangement suitable for SIMS includes anyorganized arrangement where probe spots are a consistent distance apart,ideally laid out in a precise grid pattern.

Following contacting, SIMS detection of the polymer array is performedto qualitative and/or quantitatively detect the SIMS labeled targetbound to the polymer array. Detecting can be performed with SIMSdetecting elements which comprise many SIMS instruments having aresolution of about 10 microns or less, (e.g. a ToF-SIMS). In principle,any SIMS instrument can be used to detect the presence of stableisotopes as described above provided it can rater over a sample featurebetween 13-15 micron.

In embodiments, wherein an oligonucleotide array is being used to detector sort a population of nucleic acids, the Chip-SIP approach will allowone to measure the relative amount of hybridization of the target andthe surface probe. In particular, in some of those embodiments, Chip-SIPallows relatively rapid, high sensitivity measurements of complexpopulations of target such as RNA fragments with rapid throughput andhigh resolution. As demonstrated by Applicants (see Example 5 and inFIG. 7), quantitation can be achieved with an isotopic labelconcentration as low as 0.05%.

In some embodiments, Chip-SIP also allows multiple labels to be usedsimultaneously. SIMS detection with Chip-SIP further allows forquantification of label incorporation. In some of those embodiments,Chip-SIP can be used for multiplex detection and can be used inapplications such as molecular biology and in medicine to analyze/detectmolecular recognition, e.g. hybridization between complementary strandsof DNA and other chemical and biological properties associated withmolecular recognition between biopolymers of interest.

In an embodiment, the Chip-SIP method combines polymer microarraymethodology with nano-scale secondary ion mass spectrometry (NanoSIMS)analysis. In particular, Chip-SIP can be accomplished by SIMS-labelingtargets, such as microbial nucleic acids (e.g. by exposing organismsand/or microbial communities to isotopically enriched substrates),contacting the SIMS labeled target with a polymer array configured forSIMS detection (e.g. hybridizing the SIMS labeled microbial nucleic acidto an engineered high-density oligonucleotide microarray as describedherein), and then analyzing the polymer array binding the SIMS labeledtarget through NanoSIMS.

NanoSIMS is an imaging secondary ion mass spectrometer with theunprecedented combination of high spatial resolution (50 nm), highsensitivity (1 of every 20 C/N atoms) and high mass specificity (2, 3).For example, when an ITO microarray is hybridized to isotopicallylabeled RNA fragments, the added oligonucleotides can be quantified withNanoSIMS imaging; the conductive ITO layer uniquely facilitatesgeneration of secondary ions for measurement and quantification. If thepopulation of DNA oligonucleotides are assembled as a microarray on theITO surface, a test population of complimentary nucleic acid polymers,e.g. DNA, RNA or analogs thereof (PNAs and the like) containing a stableisotope can be hybridized, and the extent of hybridization can bemeasured and quantified directly by NanoSIMS. Some of the currentmethods require substantial (15-50 atom %) enrichment of thestable-isotope, whereas Chip-SIP is able to detect small isotopicenrichments (<1 atom %). This provides for the ability to measure thelevel of isotopic enrichment of DNA/RNA hybridization to pre-synthesizedDNA array probes, which can be used for various purposes includinglinking the identity of microbes to their functional roles.

In some embodiments, CHIP-SIP can be used to: connect identity tophysiological function of microorganisms in most environmental ormedical settings (i.e. soils. sediments, lake water marine water, insectgut, human tissue) and/or to quantify hybridization or molecularrecognition events of nucleic acids on microarray surfaces. Functionalroles of microorganisms include, but are not limited to, microbialbiofilms pathogenic to human tissues, microbial communities involved inbioremediation, microorganisms controlling the fate of greenhouse gases,microbial communities present in a wide variety of engineeredbioreactors, biodegradation of pollutants, and additional functionalroles identifiable by a skilled person

In some embodiments, Chip-SIP is accomplished by isotopically-labelingmicrobial nucleic acids by exposing organisms and/or microbialcommunities to isotopically enriched substrates. The nucleic acids arethen hybridized to the engineered high-density oligonucleotidemicroarray as described herein, and then analyzed by NanoSIMS.

In several embodiments, Chip-SIP can be used to decipher of wide varietyof microbial systems having unique functional roles: microbial biofilmspathogenic to human tissues, microbial communities involved inbioremediation, microorganisms controlling the fate of greenhouse gases,microbial communities present in a wide variety of engineeredbioreactors, biodegradation of pollutants, etc.

Microbial systems refer to systems formed by microorganisms. The term“microorganism” as used herein refers to prokaryotic and eukaryoticcells, which grow as single cells, or when growing in association withother cells, do not form organs. Microorganisms include, but are notlimited to, bacteria, yeast, molds, protozoa, plankton and fungi.Exemplary microbial system that can be investigated with Chip SIPcomprise Pseudomonas stutzeri, Vibria cholera, Bacillus cereus,Francisellia tularensis, and the cellulose-degrading and N-fixingmicroorganisms found in the guts of the passalid beetle Odontotaeniusdisjunctus In an embodiment, Chip-SIP analysis can be performed onmicroorganisms that are collected from a marine and/or estuarineenvironment.

In particular in some embodiments, nucleic acid stable isotope probingtechniques (4, 5) can be used to directly connect specific substrateutilization to microbial identity. In an exemplary approach naturalmicrobial communities are incubated in the presence of substratesenriched in rare isotopes (e.g., ¹³C or ¹⁵N). The organisms, includingtheir nucleic acids, incorporate the substrate and become isotopicallyenriched over time. DNA- and RNA-Stable isotope probing techniqueexposure requires high substrate concentrations in order to meet thesensitivity threshold of density gradient separation (in manysystems >20% ¹³C DNA) (6) and can be extremely difficult to perform with¹⁵N substrates (>40% ¹⁵N DNA required) (7). Traditional SIP furtherrequires long exposure times (risking community cross-feeding),low-throughput (1-2 weeks lab processing time per sample batch), andincomplete quantification. Related culture-independent approaches canlink microbial identity to function and can also have ideal qualitiessuch as high sensitivity or in situ resolution (e.g. ¹³C-PLFA (8); ELFISH (9), FISH MAR (10), isotope arrays (11)). In contrast, the multiplestable isotope (e.g. ¹⁵N and ¹³C) incorporation made possible with theChip-SIP method combines high throughput, sensitivity, taxonomicresolution, and quantitative estimation.

Molecular approaches for detection of microbes typically targetconserved biomarkers present in all organisms of interest, such as thesmall subunit ribosomal RNA molecule (16S rRNA for prokaryotes and 18SrRNA for eukaryotes). Detection and monitoring of bacteria and archaearoutinely rely upon classifying heterogeneous 16S rRNA molecules, eitheras RNA or as gene fragments amplified by universal PCR.

In an embodiment described herein, cellular RNA is used as the nucleicacid to identify organisms because one skilled in the art wouldrecognize that the higher synthesis rates of cellular RNA allows rapidresponse to environmental stimuli.

An embodiment described herein, rRNA is used as the nucleic acid toidentify organisms. One skilled in the art would recognize that the useof rRNA facilitates the identification of organisms with higher ribosomecontent, which is the active fraction of a microbial community. Oneskilled in the art would recognize, however, that any type of natural orsynthesized nucleic acid can be used with the methods and systemdescribed herein.

As described herein, following extraction from a sample population ofinterest, isotopically hybridized nucleic acids can be hybridized to afunctional platform using probes complementary to active communitymicroorganism. Hybridization allows the identification of each probehaving a target match, as evidenced by a fluorescent signal.

In an embodiment, 16S rRNA microarrays can be used to analyze theprokaryotic composition of complex environmental samples, such as thoseobtained from bioaerosols (12), soils ((13) and water(14). Suchmicroarrays take advantage of the potential for array technology toidentify individual components and assess multiple samplessimultaneously. The 16S rRNA PhyloChip consists of almost 9,000 sets of25-mer oligonucleotide probes, and is exemplary of a type of 16S rRNAmicroarray that can be used as a functionalized platform. Each set isspecific for one 16S-rRNA gene of a particular species or group ofrelated species. Each probe set is composed of at least 11 individualperfect-match probes and their corresponding single mismatch probes,which contain one centrally located sequence mismatch. The mismatchprobe allows for the assessment and control of non-specifichybridization. For data analysis using the 16S rRNA PhyloChip, a summarystatistic that describes the quantity of sequence-specific hybridizationto each probe set can be calculated from the ratio of perfect-match tomismatch probe fluorescence for each probe and the consistency influorescence across all the probes within a given probe set.

In an embodiment, 18S DNA microarrays can be used to analyze theeukaryotic composition of complex environmental samples.

In an embodiment, hybridized mixtures of ¹³C-RNA are combined withmixtures of RNA from multiple organisms. Such an approach can provideboth a qualitative and quantitative measure (e.g. a spot can beidentified as either enriched or not, and the degree of enrichment canbe known by the heavy/light isotope ratio of the spot). Additionally,different organisms can be labeled to differing degrees, creating astandard curve of ¹³C-RNA samples, with which it can be determined thesensitivity limits and ability to generate quantitative informationbased upon the degree of isotope incorporation and thus intensity of ¹³Cin individual spots. Hybridized RNA containing stable isotopes can thenbe quantified trough SIM detection for example using NanoSIMS as hereindescribed.

As disclosed herein, the functionalized platform, probe polymers,polymer arrays and SIMS-label, can be provided as a part of systems todetect targets according to any of the methods described herein. Thesystems can be provided in the form of kits of parts.

In a kit of parts, the functionalized platform, probe polymers, polymerarrays and SIMS-label and other reagents to perform the methods can becomprised in the kit independently. One or more probe polymers andSIMS-labels can be included in one or more compositions alone or inmixtures identifiable by a skilled person. Each of the one or more ofprobe polymers or SIMS labels or other reagents can be in a compositiontogether with a suitable vehicle.

Additional reagents can include molecules suitable to enhance or favorthe contacting according to any embodiments herein described and/ormolecules, standards and/or equipment to allow detection of pressuretemperature and possibly other suitable conditions.

In particular, the components of the kit can be provided, with suitableinstructions and other necessary reagents, in order to perform themethods here described. The kit will normally contain the compositionsin separate containers. Instructions, for example written or audioinstructions, on paper or electronic support such as tapes or CD-ROMs,for carrying out the assay, will usually be included in the kit. The kitcan also contain, depending on the particular method used, otherpackaged reagents and materials (i.e. wash buffers and the like).

Further advantages and characteristics of the present disclosure willbecome more apparent hereinafter from the following detailed disclosureby way of illustration only with reference to an experimental section.

EXAMPLES

The platforms, arrays, methods and systems herein disclosed are furtherillustrated in the following examples, which are provided by way ofillustration and are not intended to be limiting.

In particular, in the following examples platforms, array and relatedmethods and systems are described that use ITO coated glass slideattaching nucleic acid probes through organosilanes or alkyl phosphonateand directed to detection of target biopolymers such as DNA or RNA. Askilled person will be able to adapt the exemplary materials, structuresand procedure to additional supports, conductive material probesfunctionalized linker probes and targets in accordance with the presentdisclosure.

Example 1 Custom Conductive Surface for Microarrays

A custom conductive surface for the microarrays is used to eliminatecharging during SIMS analysis. Glass slides coated with indium-tin oxide(ITO; Sigma) are treated with an amino- or hydroxy-alkyl phosphonate toprovide a starting matrix for DNA synthesis (FIG. 1).

Custom-designed microarrays (feature size=15 μm) are synthesized using aphotolabile deprotection strategy (15) on the LLNL Maskless ArraySynthesizer (MAS)(Roche Nimblegen, Madison, Wis.). Reagents forsynthesis (Roche Nimblegen) are delivered via an automated DNAsynthesizer (Expedite, PerSeptive Biosystems). For quality control (todetermine that DNA synthesis was successful), each slide contains a setof DNA probes to Arabidopsis calmodulin protein kinase 6 (CPK6); thelatter is detected using complimentary oligonucleotides labeled with Cy3(Integrated DNA Technologies).

If synthesis is successful, hybridization with Cy3 or Cy5-labeledcomplimentary targets reveals a series of ordered fiducial marks (probespots with the complementary sequence synthesized throughout the arrayarea). Probes targeting microbial taxa are arranged in a densely packedformation to decrease the total area analyzed by imaging secondary ionmass spectrometry the NanoSIMS. Hybridized arrays are later analyzedusing a Cameca NanoSIMS 50 which provides the critical capacity todetect isotopic enrichment in the captured ribosomal RNA fragments.

Example 2 Target RNA Extraction, Labelingand Subsequent ArrayHybridization

RNA from pelleted cells (for pure culture laboratory strains) andfilters (for aquatic field samples) are extracted with the Qiagen RNEasykit according to manufacturer's instructions, with slight modificationsfor field samples.

This protocol was used for pure cultures of P. stutzeri, V cholera andB. cereus, and it has also worked for the complex communities found inseawater and insect hindguts.

Filters are incubated in 200 μL TE buffer with 5 mg mL⁻¹ lysozyme andvortexed for 10 min at RT. RLT buffer (800 μL, Qiagen) is then added,vortexed, centrifuged, and the supernatant transferred to a new tube.Ethanol (560 μl) is added, mixed gently, and the sample is applied tothe kit-provided mini-column.

The remaining manufacturer's protocol is subsequently followed. At thispoint, RNA samples are split: one fraction saved for fluorescentlabeling (see below), the other saved unlabeled for NanoSIMS analysis.This procedure is used because the labeling protocol introducesbackground carbon (mostly ¹²C) that dilutes the ¹³C signal (data notshown). Alexafluor 546 labeling is done with the Ulysis kit (Invitrogen)for 10 min at 90° C. (2 μL RNA, 10 μL labeling buffer, 2 μL Alexafluorreagent), followed by fragmentation. All RNA (fluorescently labeled ornot) is fragmented using 5× fragmentation buffer (Affymetrix) for 10 minat 90° C. before hybridization. Labeled RNA is purified using aSPIN-OUT™mini-column (Millipore), and RNA is concentrated by ethanolprecipitation to a final concentration of 500 ng μL⁻¹.

For array hybridization, RNA samples in 1× Hybridization buffer(Nimblegen) are placed on Nimblegen X4 mixer slides and incubated insidea Maui hybridization system (BIOMICRO® Systems) for 18 hrs at 42° C. andsubsequently washed according to manufacturer's instructions(Nimblegen). Arrays with fluorescently labeled RNA are imaged with aGenepix 4000B fluorescence scanner at pmt=650 units. Arrays with RNAthat is not fluorescently labeled are marked with a diamond pen and alsoimaged with the fluorescence scanner to subsequently navigate to theanalysis spots in the NanoSIMS.

These spots are observable in the fluorescence image because fiducialprobe spots are synthesized around the outline of the area to beanalyzed by NanoSIMS. Prior to NanoSIMS analysis, samples are not metalcoated to avoid further dilution of the RNA's isotope ratio or loss ofmaterial Finally, slides are trimmed and mounted in custom-builtstainless steel holders.

Example 3 NanoSIMS Analysis

Secondary ion mass spectrometry analysis of microarrays hybridized with¹³C and/or ¹⁵N rRNA is performed with a Cameca NanoSIMS 50 (Cameca,Gennevilliers, France).

A Cs+ primary ion beam is used to enhance the generation of negativesecondary ions. Carbon and nitrogen isotopic ratios are determined byelectrostatic peak switching on electron multipliers in pulse countingmode, alternately measuring ¹²C¹⁴N⁻ and ¹²C¹⁵N⁻ simultaneously for the¹⁵N/¹⁴N ratio, and then measuring ¹²C¹⁴N⁻ and ¹³C¹⁴N⁻ and simultaneouslyfor the ¹³C/¹²C ratio. Peak switching strategy is used because thesecondary ion count rate for the CN⁻ species in these samples is 5-10times higher than any of the other carbon species (e.g., C⁻, CH⁻, C₂ ⁻),and therefore higher precision is achieved even though total analyticaltime is split between the two CN⁻ species at mass 27.

If only one isotopic ratio was needed, peak switching was not performed.Mass resolution is set to ˜10,000 mass resolving power to minimize thecontribution of isobaric interferences to the species of interest (e.g.,¹¹B¹⁶O⁻ contribution to ¹³C¹⁴N⁻<1/100; ¹³C₂ ⁻ contribution to¹²C¹⁴N⁻<1/1000). Analyses are performed in imaging mode to generatedigital ion images of the sample for each ion species. Analyticalconditions are optimized for speed of analysis, ability to spatiallyresolve adjacent hybridization locations, and analytical stability. Theprimary beam current is set to 5 to 7 pA Cs⁺, which yields spatialresolution of 200-400 nm and a maximum count rate on the detectors of˜300,000 cps ¹²C¹⁴N. Analysis area is 50×50 μm² with a pixel density of256×256 with 0.5 or 1 ms/pixel dwell time. For peak switching, one scanof the analysis area is made per species set, resulting in two scans peranalytical cycle. With these conditions, reproducible secondary ionratios can be measured for a maximum of 4 cycles through the two sets ofmeasurements before the sample is largely consumed.

Data are collected for 2 to 4 cycles. Based on total counts for analyzedcycles, precision of 2-3% for ¹³C¹⁴N and 1-4% for ¹⁵N¹²C can be achieveddepending on the enrichment and hybridization intensity. A singlemicroarray analysis of approximately 2500 probes, with an area of 0.75mm² and the acquisition of 300 images, was carried out using the Camecasoftware automated chain analysis in 16 hours. Ion images are stitchedtogether and processed to generate isotopic ratios with custom software(L'IMAGE, L. Nittler, Carnegie Institution of Washington). Ion countsare corrected for detector dead time on a pixel by pixel basis.

Hybridization locations are selected by hand or with the auto-ROIfunction, and isotopic ratios are calculated for the selected regionsover all cycles to produce the location isotopic ratios. Isotopic ratiosare converted to delta values using δ=[(R_(meas)/R_(standard)) 1]×1000,where R the measured ratio and R_(standard) is the standard ratio(0.00367 for ¹⁵N/¹⁴N and 0.011237 for Data are corrected for naturalabundance ratios measured in unhybridized locations of the sample.

Example 4 Detection and Data Analysis

For each taxon identified by a microarray probe spot, isotopicenrichment of individual probe spots is plotted against fluorescence andthe linear regression slope is calculated with the y-interceptconstrained to natural isotope abundances (zero permil for ¹⁵N data and−20 permil for ¹³C data).

This calculated slope (permil/fluorescence), referred to ashybridization-corrected enrichment (HCE), is a metric that can be usedto compare the relative incorporation of a given substrate by differenttaxa. It should be noted that due to the different naturalconcentrations of ¹³C and ¹⁵N, and more importantly, differentbackground contributions from the microarray, HCEs for ¹⁵N substratesand ¹³C substrates are not comparable.

Example 5 Applicability of Chip-SIP to Microbial Cultures

Initial tests spotted slides with synthetic DNA oligonucleotidesrepresenting/covering the genome of a strain of Francisellia tularensis,creating a DNA array. Microscopic examination of the autofluorescence ofthe arrays provides initial visual assessment of spotting efficiency andsample-substrate interaction.

The features on an alkyl phosphonate spotted array are approximately 150um in diameter (FIG. 2B), while those spotted on traditional glasssilane (coated with the industry standard y-aminopropylsilane) areroughly half the size.

The results of the test illustrated in FIG. 2A and FIG. 2B showpreliminary evaluation of glass arrays with (FIG. 2A) traditionaltriethoxy silane coating versus (FIG. 2B) new alkylphosphonate surfacechemistry., (C) ITO array has higher signal to noise for fluorescentsignal than traditional silane array. The preliminary results ofspotting DNA oligonucleotides (short pieces of DNA) for thealkylphosphonate surface suggests it has highly stable binding, andlarger, more uniform spot size. In hybridization tests withfluorescently labeled cDNA generated from F. tularensis RNA samples,signals generated from the phosphonate surface were comparable to thetriethoxy silane derivatized slide (hybridization data not shown).

A further series of experiments showed that an ITO coated array slidecan be successfully analyzed by NanoSIMS. 5 μm region of ITO coatedmicroarray was sputtered for 20 minutes. The ion plot of carbon (¹²C),oxygen (¹⁶O) and silicon (²⁸Si) generated during NanoSIMS analysis of anITO-coated microarray is illustrated in FIG. 3.

As shown in the illustration of FIG. 3, the ion concentrations change asthe array surface is sputtered by the NanoSIMS primary ion bean. ITOcoating makes this array much more conducive, and appropriate forNanoSIMS analysis, than traditional microarraysIon counts over time froma NanoSIMS depth analysis indicate the suitability of ITO surfaces forSIMS analysis (FIG. 3), as the conductive, oxide coating allows forconsistent ion sputtering for a sustained period with depth.

A skilled person will understand that the exemplary results shown inFIG. 3 provide a proof of concept for the devices methods and systemsherein described with a simple ITO coated glass slide (not yet printedwith oligos to make it an array) and not yet hybridized. In particularthe results on the conductive platform that has been analyzed by SIMSshow minimization of charges build up and a cleaner signal with respectto certain other traditional array of the art.

The suitability and stability of ITO coated arrays for SIMS analysis aredemonstrated with a further series of experiments resulting in NanoSIMSanalysis images following sputtering of a 5 μm region for 20 minutes.

The results illustrated in FIG. 4). show no evidence of sample charging(as there is with an uncoated, standard microarray). These exemplaryresult provides a proof of concept with a simple ITO coated glass slide(not yet printed with oligos to make it an array) and not yet hybridizedas would be understood by a skilled person.

In further proof of concept experiments, after extracting RNA frommicrobial cultures of Pseudomonas stuzeri exposed to ¹³C glucose,NanoSIMS was used to detect isotopic enrichment in P. stuzeri rRNAhybridized to oligonucleotide probe spots on a microarray.

The results of hybridization of extracted RNA from a single bacterialspecies (Pseudomonas stutzeri) grown on ¹³C-glucose as the sole carbonsource are illustrated in FIG. 5A (fluorescence microarray scanner),FIG. 5B (¹³C enrichment by NanoSIMS) and FIG. 5C (Plot of ¹³C enrichmentby NanoSIMS versus fluorescence by microarray scanner). Each spot (anddata point) represents a distinct probe specific for Pseudomonas. P.stuzeri isolates were grown on 99 atom % ¹³C-glucose until fully isotopelabeled. RNA was extracted and hybridized to a microarray containingprobe sets designed to hybridize to P. stuzeri. Mismatch probes weresynthesized as negative controls.

By imaging multiple probe spots simultaneously with the NanoSIMS,isotopically enriched nucleic acids were identified against the largebackground of non-enriched genes in a mixed microbial community RNAsample (FIG. 5B). The excellent correlation (FIG. 5C) of these data(fluorescence (a measure of how much RNA is hybridized) and ¹³Cenrichment) to the standard fluorescence analysis of the array (FIG. 5A)demonstrates successful detection of labeled RNA by NanoSIMS.

NanoSIMS measurements demonstrate detection of ¹³C in successfullyhybridized probe spots (FIG. 5A and FIG. 5B). Fluorescence is a measureof how much RNA is hybridized, which is positively correlated with ¹³Cenrichment, demonstrating successful detection of labeled RNA byNanoSIMS (FIG. 5C).

Results of RNA hybridization to ITO microarray and then detection viaNanoSIMS illustrated in FIG. 6 also show that RNA enriched with 0.5% ¹³Cis successfully detected by the Chip-SIP approach (see in particularFIG. 6A Fluorescence by microarray scanner; and FIG. 6B ¹³C enrichmentby nanoSIMS.)

To demonstrate that the approach works with mixtures of nucleic acids,enriched to differing degrees, isolates of two bacterial strains (Vibriocholerae and Bacillus cereus) were grown on multiple differentenrichment levels of ¹³C glucose. Fluorescence and NanoSIMS analysis ofthe mixed ¹³C and ¹⁵N V. cholerae and B. cereus RNA on ITO arrayshybridized with differential isotopic enrichment shows clear separationof the two different RNA types (FIG. 7A and FIG. 7B). A useful parameterfor comparing the two taxa's RNA is “HCE”, or hybridization correctedenrichment, a metric which allows different populations of RNA moleculesto be compared with respect to their isotopic enrichment (FIG. 7). Theanalysis of ¹³C and ¹⁵N in two different types of RNA with differentialisotopic enrichment illustrated in FIG. 7A, and FIG. 7B—demonstratesclear separation of the two different types.

This exemplary series of experiments demonstrates that the Chip-SIPmethod works with mixtures of RNA (from different taxa) and also withmixtures of both low and high isotope enrichment.

Additional experiments with simple two-member communities includingPseudomonas stutzeri grown on 25% ¹⁵N ammonium and Bacillus cereus grownon natural abundance ammonium demonstrate that unenriched RNA is notdetected via false positive measurements. From each culture, RNA wasextracted, mixed in equal concentrations, and hybridized to anITO-coated array. Array fluorescence (FIG. 8A) and d¹⁵N by nanoSIMS(FIG. 8B) measurements prove that unlabeled taxa do not show isotopicsignal in NanoSIMS images, and the Chip-SIP method is quantitative (e.g.one taxon is more enriched than another).

The results illustrated in FIG. 8 show as a control that unlabeled taxado not show isotopic signals in NanoSIMS. This set of resultsdemonstrates that the NanoSIMS analysis is quantitative (e.g. one taxonis more enriched than another) and that non-specific binding is notoccurring on the arrays—otherwise isotopic enrichment would be evidentin the region of ¹²C (Bacillus cereus) probes at the bottom of thefigure. Additionally unlabeled taxa do not show isotopic signal inNanoSIMS analyses.

Additional experiments with simple two-member communities includingPseudomonas stutzeri grown on 100% ¹³C glucose and Vibrio cholera grownon 20% ¹³C glucose demonstrate that two different types of RNA, enrichedto different levels and mixed, can be statistically separated with theChip-SIP method (FIG. 9). A one way ANOVA analyzing the two populationsof data is significant (p<0.0001). These experiments prove thatunlabeled taxa do not show isotopic signal in NanoSIMS, and that theChip-SIP method is quantitative (e.g. proving one taxon is more enrichedthan another).

Additionally, the experiments of FIG. 9 demonstrates that unlabeled taxado not show isotopic signal in the NanoSIMS analysis of their RNAhybridized to an ITO microarray, and the Chip-SIP method is quantitative(e.g. one taxon is more enriched than another).

Example 6 Use of Chip-Sip to Identify Resource Utilization in ComplexMicrobial Communities

The Chip-SIP method of analyzing isotopic or elementally labeled RNAfragments on a high density ITO microarray, can be particularly usefulwhen applied to naturally occurring environmental microbes in which a16S rRNA and 18S rRNA microarray for common marine microbial taxa(bacteria, archaea, and protists) has been designed to target specificphylotypes (approximately at the species/genus level). In such cases,the technique allows simultaneous identification of a taxa's identityand its physiology.

Currently little is known about organic carbon incorporation patterns inmarine and estuarine environments, partly because the dominant organismsare uncultured and cannot be directly interrogated in the laboratory.Applicants used the Chip-SIP method to test whether different taxaincorporate amino acids, fatty acids, and starch for their carbon growthrequirements.

A Target taxa selection was performed by PhyloChip analysis and de novoprobe design. RNA extracts from SF Bay SIP experiment samples weretreated with DNAse I and reverse-transcribed to produce cDNA using theGenechip Expression 3′ amplification one-cycle cDNA synthesis kit(Affymetrix). The cDNA was PCR amplified with bacterial and archaealprimers, fragmented, fluorescently labeled, and hybridized to the G2PhyloChip (6). Taxa (16S operating taxonomic units, OTU) considered tobe present in the samples were identified based on 90% of the probes forthat taxon being responsive, defined as the signal of the perfect matchprobe >1.3 times the signal from the mismatch probe. From approximately1500 positively identified taxa, we chose a subset of 100 taxa commonlyfound in marine environments to target with chip-SIP. We also did nottarget OTUs previously identified from soil, sewage, and bioreactors asour goal was to characterize the activity of marine microorganisms.Using the Greengenes database (18) implemented in ARB (19), Applicantsdesigned 25 probes (25 by long), to create a ‘probe set’ for each taxon(see SEQ ID NO: 1 to SEQ ID NO: 2805 of the annexed Sequence Listingincorporated herein by reference in its entirety), as well as generalprobes for the three domains of life. Probes for single laboratorystrains (Pseudomonas stutzeri, Bacillus cereus, and Vibrio cholerae)were also designed with ARB (SEQ ID NO: 1 to SEQ ID NO: 2805 of theannexed Sequence Listing incorporated herein by reference in itsentirety). Sequences of the probes are also reported in the followingtable

TABLE 1 list of probes specific for laboratory bacterial strainsSEQUENCE_ID PROBE_SEQUENCE SEQUENCE_ID PROBE_SEQUENCE SEQUENCE_IDPROBE_SEQUENCE Pstutzeri_1 TAACCGTCCCCCCGAAG Vcholerae_1 AACTTAACCACCTTCBcereus_1 TCCACCTCGCGGTCTT GTTAGACT CTCCCTACTG GCAGCTCTT Pstutzeri_2GGTAACCGTCCCCCCGA Vcholerae_2 GTAGGTAACGTCAA Bcereus_2 GCCTTTCAATTTCGAAAGGTTAGA ATGATTAAGGT CCATGCGGT Pstutzeri_3 TGGTAACCGTCCCCCCG Vcholerae_3TGTAGGTAACGTCA Bcereus_3 CTCTTAATCCATTCGC AAGGTTAG AATGATTAAGG TCGACTTGCPstutzeri_4 GTAACCGTCCCCCCGAA Vcholerae_4 TAACTTAACCACCTT Bcereus_4CCACCTCGCGGTCTTG GGTTAGAC CCTCCCTACT CAGCTCTTT Pstutzeri_5ACTCCGTGGTAACCGTC Vcholerae_5 ACTTAACCACCTTCC Bcereus_5 CTCTGCTCCCGAAGGCCCCCGAA TCCCTACTGA AGAAGCCCTA Pstutzeri_6 CACTCCGTGGTAACCGT Vcholerae_6TTAACTTAACCACCT Bcereus_6 CCGCCTTTCAATTTCG CCCCCCGA TCCTCCCTAC AACCATGCGPstutzeri_7 TCACTCCGTGGTAACCG Vcholerae_7 TAAGGTATTAACTTA Bcereus_7TCTGCTCCCGAAGGA TCCCCCCG ACCACCTTCC GAAGCCCTAT Pstutzeri_8ACCGTCCCCCCGAAGGT Vcholerae_8 CTGTAGGTAACGTC Bcereus_8 ACCTGTCACTCTGCTCTAGACTAG AAATGATTAAG CCGAAGGAG Pstutzeri_9 ATCACTCCGTGGTAACC Vcholerae_9CTTAACCACCTTCCT Bcereus_9 GCTCTTAATCCATTCG GTCCCCCC CCCTACTGAA CTCGACTTGPstutzeri_10 CCGTGGTAACCGTCCCC Vcholerae_10 ATTAACTTAACCAC Bcereus_10CGCCTTTCAATTTCGA CCGAAGGT CTTCCTCCCTA ACCATGCGG Pstutzeri_11CTCCGTGGTAACCGTCC Vcholerae_11 AAGGTATTAACTTA Bcereus_11 ACTCTGCTCCCGAAGCCCCGAAG ACCACCTTCCT GAGAAGCCCT Pstutzeri_12 CCGTCCCCCCGAAGGTTVcholerae_12 TTAACCACCTTCCTC Bcereus_12 GCTCCCGAAGGAGAA AGACTAGCCCTACTGAAA GCCCTATCTC Pstutzeri_13 CCACCACCCTCTGCCAT Vcholerae_13CTTCTGTAGGTAACG Bcereus_13 TCACTCTGCTCCCGAA ACTCTAGC TCAAATGATTGGAGAAGCC Pstutzeri_14 TCCACCACCCTCTGCCA Vcholerae_14 TATTAACTTAACCACBcereus_14 TCTTAATCCATTCGCT TACTCTAG CTTCCTCCCT CGACTTGCA Pstutzeri_15TTCCACCACCCTCTGCC Vcholerae_15 ACGACGTACTTTGTG Bcereus_15CTGCTCCCGAAGGAG ATACTCTA AGATTCGCTC AAGCCCTATC Pstutzeri_16AATTCCACCACCCTCTG Vcholerae_16 TACGACGTACTTTGT Bcereus_16TAATCCATTCGCTCGA CCATACTC GAGATTCGCT CTTGCATGT Pstutzeri_17AAATTCCACCACCCTCT Vcholerae_17 ACTACGACGTACTTT Bcereus_17CACTCTGCTCCCGAA GCCATACT GTGAGATTCG GGAGAAGCCC Pstutzeri_18GAAATTCCACCACCCTC Vcholerae_18 CTACGACGTACTTTG Bcereus_18GGTCTTGCAGCTCTTT TGCCATAC TGAGATTCGC GTACCGTCC Pstutzeri_19ATTCCACCACCCTCTGC Vcholerae_19 GACTACGACGTACT Bcereus_19 TGCTCCCGAAGGAGACATACTCT TTGTGAGATTC AGCCCTATCT Pstutzeri_20 GGAAATTCCACCACCCTVcholerae_20 AGGTATTAACTTAA Bcereus_20 CTTAATCCATTCGCTC CTGCCATACCACCTTCCTC GACTTGCAT Pstutzeri_21 CAGGAAATTCCACCACC Vcholerae_21GGTATTAACTTAACC Bcereus_21 TTAATCCATTCGCTCG CTCTGCCA ACCTTCCTCCACTTGCATG Pstutzeri_22 AGGAAATTCCACCACCC Vcholerae_22 GTATTAACTTAACCABcereus_22 CTCCCGAAGGAGAAG TCTGCCAT CCTTCCTCCC CCCTATCTCT Pstutzeri_23CAGTGTCAGTATTAGCC Vcholerae_23 CGCGGTATCGCTGC Bcereus_23GTCACTCTGCTCCCGA CAGGTGGT CCTCTGTATAC AGGAGAAGC Pstutzeri_24TCAGTATTAGCCCAGGT Vcholerae_24 TCGCGGTATCGCTG Bcereus_24CACCTCGCGGTCTTGC GGTCGCCT CCCTCTGTATA AGCTCTTTG Pstutzeri_25TCAGTGTCAGTATTAGC Vcholerae_25 CTTGTCAGTTTCAAA Bcereus_25GTCTTGCAGCTCTTTG CCAGGTGG TGCGATTCCT TACCGTCCA Pstutzeri_26TGTCAGTATTAGCCCAG Vcholerae_26 TTGTCAGTTTCAAAT Bcereus_26TGTCACTCTGCTCCCG GTGGTCGC GCGATTCCTA AAGGAGAAG Pstutzeri_27GTCAGTATTAGCCCAGG Vcholerae_27 GCGGTATCGCTGCC Bcereus_27 TCCCGAAGGAGAAGCTGGTCGCC CTCTGTATACG CCTATCTCTA Pstutzeri_28 CCTCAGTGTCAGTATTAVcholerae_28 CCTGGGCATATCCG Bcereus_28 CGGTCTTGCAGCTCTT GCCCAGGTGTAGCGCAAGG TGTACCGTC Pstutzeri_29 CTCAGTGTCAGTATTAG Vcholerae_29TCCCACCTGGGCAT Bcereus_29 TCAAAATGTTATCCG CCCAGGTG ATCCGGTAGCGGTATTAGCCC Pstutzeri_30 ACCTCAGTGTCAGTATT Vcholerae_30 GGCATATCCGGTAGBcereus_30 CCTGTCACTCTGCTCC AGCCCAGG CGCAAGGCCCG CGAAGGAGA Pstutzeri_31GTGTCAGTATTAGCCCA Vcholerae_31 ACCTGGGCATATCC Bcereus_31TTCAAAATGTTATCCG GGTGGTCG GGTAGCGCAAG GTATTAGCC Pstutzeri_32AGTGTCAGTATTAGCCC Vcholerae_32 CTGGGCATATCCGG Bcereus_32CACCTGTCACTCTGCT AGGTGGTC TAGCGCAAGGC CCCGAAGGA Pstutzeri_33CACCTCAGTGTCAGTAT Vcholerae_33 CCCACCTGGGCATA Bcereus_33TCTTGCAGCTCTTTGT TAGCCCAG TCCGGTAGCGC ACCGTCCAT Pstutzeri_34GCACCTCAGTGTCAGTA Vcholerae_34 TGGGCATATCCGGT Bcereus_34CTGTCACTCTGCTCCC TTAGCCCA AGCGCAAGGCC GAAGGAGAA Pstutzeri_35CGCACCTCAGTGTCAGT Vcholerae_35 GGGCATATCCGGTA Bcereus_35GCGGTCTTGCAGCTCT ATTAGCCC GCGCAAGGCCC TTGTACCGT Pstutzeri_36TTCGCACCTCAGTGTCA Vcholerae_36 GCATATCCGGTAGC Bcereus_36 CGCGGTCTTGCAGCTGTATTAGC GCAAGGCCCGA CTTTGTACCG Pstutzeri_37 TCGCACCTCAGTGTCAGVcholerae_37 CCACCTGGGCATAT Bcereus_37 AGCTCTTAATCCATTC TATTAGCCCCGGTAGCGCA GCTCGACTT Pstutzeri_38 AATGCGTTAGCTGCGCC Vcholerae_38CATATCCGGTAGCG Bcereus_38 ACCTCGCGGTCTTGC ACTAAGAT CAAGGCCCGAAAGCTCTTTGT Pstutzeri_39 CACCACCCTCTGCCATA Vcholerae_39 CACCTGGGCATATCBcereus_39 TCGCGGTCTTGCAGCT CTCTAGCT CGGTAGCGCAA CTTTGTACC Pstutzeri_40ACACAGGAAATTCCACC Vcholerae_40 ATATCCGGTAGCGC Bcereus_40 CTCGCGGTCTTGCAGACCCTCTG AAGGCCCGAAG CTCTTTGTAC Pstutzeri_41 CACAGGAAATTCCACCAVcholerae_41 TATCCGGTAGCGCA Bcereus_41 TGCACCACCTGTCACT CCCTCTGCAGGCCCGAAGG CTGCTCCCG Pstutzeri_42 ACAGGAAATTCCACCAC Vcholerae_42TCCCCTGCTTTGCTC Bcereus_42 ATGCACCACCTGTCA CCTCTGCC TTGCGAGGTTCTCTGCTCCC Pstutzeri_43 GAAGTTAGCCGGTGCTT Vcholerae_43 GTCCCCTGCTTTGCTBcereus_43 ACCACCTGTCACTCTG ATTCTGTC CTTGCGAGGT CTCCCGAAG Pstutzeri_44GAAAGTTCTCTGCATGT Vcholerae_44 CCGAAGGTCCCCTG Bcereus_44 GCACCACCTGTCACTCAAGGCCT CTTTGCTCTTG CTGCTCCCGA Pstutzeri_45 AAAGTTCTCTGCATGTCVcholerae_45 GGTCCCCTGCTTTGC Bcereus_45 CACCACCTGTCACTCT AAGGCCTGTCTTGCGAGG GCTCCCGAA Pstutzeri_46 TCTCTGCATGTCAAGGC Vcholerae_46GAAGGTCCCCTGCT Bcereus_46 CATAAGAGCAAGCTC CTGGTAAG TTGCTCTTGCGTTAATCCATT Pstutzeri_47 GTTCTCTGCATGTCAAG Vcholerae_47 AGGTCCCCTGCTTTGBcereus_47 CCTCGCGGTCTTGCA GCCTGGTA CTCTTGCGAG GCTCTTTGTA Pstutzeri_48AGTTCTCTGCATGTCAA Vcholerae_48 CGAAGGTCCCCTGC Bcereus_48CCACCTGTCACTCTGC GGCCTGGT TTTGCTCTTGC TCCCGAAGG Pstutzeri_49AAGTTCTCTGCATGTCA Vcholerae_49 AAGGTCCCCTGCTTT Bcereus_49AAGAGCAAGCTCTTA AGGCCTGG GCTCTTGCGA ATCCATTCGC Pstutzeri_50CTCTGCATGTCAAGGCC Vcholerae_50 CCCCTGCTTTGCTCT Bcereus_50CGAAGGAGAAGCCCT TGGTAAGG TGCGAGGTTA ATCTCTAGGG Pstutzeri_51TTCTCTGCATGTCAAGG Vcholerae_51 TCTAGGGCACAACC Bcereus_51AAGCTCTTAATCCATT CCTGGTAA TCCAAGTAGAC CGCTCGACT Pstutzeri_52CTGCATGTCAAGGCCTG Vcholerae_52 CTCTAGGGCACAAC Bcereus_52 TAAGAGCAAGCTCTTGTAAGGTT CTCCAAGTAGA AATCCATTCG Pstutzeri_53 TCTGCATGTCAAGGCCTVcholerae_53 CCTCTAGGGCACAA Bcereus_53 ATAAGAGCAAGCTCT GGTAAGGTCCTCCAAGTAG TAATCCATTC Pstutzeri_54 TACTCACCCGTCCGCCG Vcholerae_54CGACGTACTTTGTGA Bcereus_54 CCCGAAGGAGAAGCC CTGAATCA GATTCGCTCCCTATCTCTAG Pstutzeri_55 CAGCCATGCAGCACCTG Vcholerae_55 TCAGTTTCAAATGCGBcereus_55 CCGAAGGAGAAGCCC TGTCAGAG ATTCCTAGGT TATCTCTAGG Pstutzeri_56ACAGCCATGCAGCACCT Vcholerae_56 AGTTTCAAATGCGA Bcereus_56CAAGCTCTTAATCCAT GTGTCAGA TTCCTAGGTTG TCGCTCGAC Pstutzeri_57GACAGCCATGCAGCAC Vcholerae_57 TGTCAGTTTCAAATG Bcereus_57 AAGGAGAAGCCCTATCTGTGTCAG CGATTCCTAG CTCTAGGGTT Pstutzeri_58 CTGGAAAGTTCTCTGCAVcholerae_58 GTTTCAAATGCGATT Bcereus_58 GAAGGAGAAGCCCTA TGTCAAGGCCTAGGTTGA TCTCTAGGGT Pstutzeri_59 TGGAAAGTTCTCTGCAT Vcholerae_59CTAGCTTGTCAGTTT Bcereus_59 GCAAGCTCTTAATCC GTCAAGGC CAAATGCGATATTCGCTCGA Pstutzeri_60 GGAAAGTTCTCTGCATG Vcholerae_60 TCTAGCTTGTCAGTTBcereus_60 AGCAAGCTCTTAATC TCAAGGCC TCAAATGCGA CATTCGCTCG eukaryotes_1AACTAAGAACGGCCAT sphingo_1_1 CCAGCTTGCTGCCCT alpha_7_1 ACATCTCTGTTTCCGCGCACCACCA CTGTACCATC GACCGGGAT eukaryotes_2 CACCAACTAAGAACGG sphingo_1_2CAGCTTGCTGCCCTC alpha_7_2 CATCTCTGTTTCCGCG CCATGCACC TGTACCATCCACCGGGATG eukaryotes_3 CCAACTAAGAACGGCC sphingo_1_3 GCCAGCTTGCTGCCalpha_7_3 AAACATCTCTGTTTCC ATGCACCAC CTCTGTACCAT GCGACCGGG eukaryotes_4ACCAACTAAGAACGGC sphingo_1_4 TGCCAGCTTGCTGCC alpha_7_4 GAAACATCTCTGTTTCCATGCACCA CTCTGTACCA CGCGACCGG eukaryotes_5 CCACCAACTAAGAACG sphingo_1_5CAGTTTACGACCCA alpha_7_5 AGAAACATCTCTGTTT GCCATGCAC GAGGGCTGTCTCCGCGACCG eukaryotes_6 TCCACCAACTAAGAACG sphingo_1_6 AGCAGTTTACGACCalpha_7_6 AACATCTCTGTTTCCG GCCATGCA CAGAGGGCTGT CGACCGGGA eukaryotes_7CAACTAAGAACGGCCA sphingo_1_7 AAGCAGTTTACGAC alpha_7_7 ATCTCTGTTTCCGCGATGCACCACC CCAGAGGGCTG CCGGGATGT eukaryotes_8 CTCCACCAACTAAGAACsphingo_1_8 GCAGTTTACGACCC alpha_7_8 CTGCCACTGTCCACCC GGCCATGCAGAGGGCTGTC GAGCAAGCT eukaryotes_9 TTGGAGCTGGAATTACC sphingo_1_9CCGCCTACCTCTAGT alpha_7_9 CCACTGTCCACCCGA GCGGCTGC GTATTCAAGC GCAAGCTCGGeukaryotes_10 TCAGGCTCCCTCTCCGG sphingo_1_10 CATTCCGCCTACCTC alpha_7_10GCCACTGTCCACCCG AATCGAAC TAGTGTATTC AGCAAGCTCG eukaryotes_11TCTCAGGCTCCCTCTCC sphingo_1_11 TGCTGTTGCCAGCTT alpha_7_11AAACCTCTAGGTAGA GGAATCGA GCTGCCCTCT TACCCACGCG eukaryotes_12TATTGGAGCTGGAATTA sphingo_1_12 GCTGTTGCCAGCTTG alpha_7_12CCAAACCTCTAGGTA CCGCGGCT CTGCCCTCTG GATACCCACG eukaryotes_13ATTGGAGCTGGAATTAC sphingo_1_13 TTGCTGTTGCCAGCT alpha_7_13GTCTGCCACTGTCCAC CGCGGCTG TGCTGCCCTC CCGAGCAAG eukaryotes_14TAAGAACGGCCATGCA sphingo_1_14 CACATTCCGCCTACC alpha_7_14 CCACCCGAGCAAGCTCCACCACCC TCTAGTGTAT CGGGTTTCTC eukaryotes_15 CTAAGAACGGCCATGCsphingo_1_15 GTCACATTCCGCCTA alpha_7_15 TGCCACTGTCCACCC ACCACCACCCCTCTAGTGT GAGCAAGCTC eukaryotes_16 ACTAAGAACGGCCATG sphingo_1_16TCACATTCCGCCTAC alpha_7_16 CAAACCTCTAGGTAG CACCACCAC CTCTAGTGTAATACCCACGC eukaryotes_17 CTCAGGCTCCCTCTCCG sphingo_1_17 GCTTTCGCTTAGCCGalpha_7_17 TCTGCCACTGTCCACC GAATCGAA CTAACTGTGT CGAGCAAGC eukaryotes_18CTATTGGAGCTGGAATT sphingo_1_18 CGCTTTCGCTTAGCC alpha_7_18CGTCTGCCACTGTCCA ACCGCGGC GCTAACTGTG CCCGAGCAA eukaryotes_19AAGAACGGCCATGCAC sphingo_1_19 TCGCTTAGCCGCTA alpha_7_19 TCCGAACCTCTAGGTCACCACCCA ACTGTGTATCG AGATTCCCAC eukaryotes_20 AGGCTCCCTCTCCGGAAsphingo_1_20 TTCGCTTAGCCGCTA alpha_7_20 CACCCGAGCAAGCTC TCGAACCCACTGTGTATC GGGTTTCTCG eukaryotes_21 CAGGCTCCCTCTCCGGA sphingo_1_21CTTTCGCTTAGCCGC alpha_7_21 ACCCGAGCAAGCTCG ATCGAACC TAACTGTGTAGGTTTCTCGT eukaryotes_22 GCTATTGGAGCTGGAAT sphingo_1_22 CTGTTGCCAGCTTGCalpha_7_22 CCGTCTGCCACTGTCC TACCGCGG TGCCCTCTGT ACCCGAGCA eukaryotes_23TTTCTCAGGCTCCCTCT sphingo_1_23 GTTGCCAGCTTGCTG alpha_7_23CCGAACCTCTAGGTA CCGGAATC CCCTCTGTAC GATTCCCACG eukaryotes_24GGCTCCCTCTCCGGAAT sphingo_1_24 TGTTGCCAGCTTGCT alpha_7_24AACCTCTAGGTAGAT CGAACCCT GCCCTCTGTA ACCCACGCGT eukaryotes_25CACTCCACCAACTAAGA sphingo_1_25 CGCTTAGCCGCTAA alpha_7_25 TCCACCCGAGCAAGCACGGCCAT CTGTGTATCGC TCGGGTTTCT archaea_1 TTGTGGTGCTCCCCCGC sphingo_2_1TCACCGCTACACCC alpha_8_1 CTGCCACTGTCCACCC CAATTCCT CTCGTTCCGCT GAGCAAGCTarchaea_2 TGCTCCCCCGCCAATTC sphingo_2_2 GCTATCGGCGTTCTG alpha_8_2GCCACTGTCCACCCG CTTTAAGT AGGAATATCT AGCAAGCTCG archaea_3CGCGCCTGCTGCGCCCC sphingo_2_3 CGCTATCGGCGTTCT alpha_8_3 AAACCTCTAGGTAGAGTAGGGCC GAGGAATATC TACCCACGCG archaea_4 TTTCGCGCCTGCTGCGC sphingo_2_4TCGGCGTTCTGAGG alpha_8_4 GTCTGCCACTGTCCAC CCCGTAGG AATATCTATGC CCGAGCAAGarchaea_5 TCGCGCCTGCTGCGCCC sphingo_2_5 TTCACCGCTACACCC alpha_8_5CCACCCGAGCAAGCT CGTAGGGC CTCGTTCCGC CGGGTTTCTC archaea_6TTCGCGCCTGCTGCGCC sphingo_2_6 TTTCACCGCTACACC alpha_8_6 TGCCACTGTCCACCCCCGTAGGG CCTCGTTCCG GAGCAAGCTC archaea_7 GTGCTCCCCCGCCAATT sphingo_2_7TCGCTTTCGCTTAGC alpha_8_7 CAAACCTCTAGGTAG CCTTTAAG CACTTACTGT ATACCCACGCarchaea_8 GCTCCCCCGCCAATTCC sphingo_2_8 CGGCGTTCTGAGGA alpha_8_8TCTGCCACTGTCCACC TTTAAGTT ATATCTATGCA CGAGCAAGC archaea_9GCGCCTGCTGCGCCCCG sphingo_2_9 AACTAATGGGGCGC alpha_8_9 ACTGTCCACCCGAGCTAGGGCCT ATGCCCATCCC AAGCTCGGGT archaea_10 CGCCTGCTGCGCCCCGTsphingo_2_10 CGCTTAGCCACTTAC alpha_8_10 CCACTGTCCACCCGA AGGGCCTGTGTATATCGC GCAAGCTCGG archaea_11 GCCTGCTGCGCCCCGTA sphingo_2_11ACTAATGGGGCGCA alpha_8_11 CCAAACCTCTAGGTA GGGCCTGG TGCCCATCCCGGATACCCACG archaea_12 GTTTCGCGCCTGCTGCG sphingo_2_12 GCCATGCAGCACCTalpha_8_12 GTCCACCCGAGCAAG CCCCGTAG CGTATAGAGTC CTCGGGTTTC archaea_13CTTGTGGTGCTCCCCCG sphingo_2_13 AGCCATGCAGCACC alpha_8_13 TCCACCCGAGCAAGCCCAATTCC TCGTATAGAGT TCGGGTTTCT archaea_14 GGTTTCGCGCCTGCTGCsphingo_2_14 CAGCCATGCAGCAC alpha_8_14 CGTCTGCCACTGTCCA GCCCCGTACTCGTATAGAG CCCGAGCAA archaea_15 AGGTTTCGCGCCTGCTG sphingo_2_15ACAGCCATGCAGCA alpha_8_15 TGTCCACCCGAGCAA CGCCCCGT CCTCGTATAGAGCTCGGGTTT archaea_16 CCTGCTGCGCCCCGTAG sphingo_2_16 CTTACTTGTCAGCCTalpha_8_16 ACCTCTAGGTAGATA GGCCTGGA ACGCACCCTT CCCACGCGTT archaea_17CCTTGTGGTGCTCCCCC sphingo_2_17 ACTTACTTGTCAGCC alpha_8_17CACCCGAGCAAGCTC GCCAATTC TACGCACCCT GGGTTTCTCG archaea_18CCCCTTGTGGTGCTCCC sphingo_2_18 CCACTGACTTACTTG alpha_8_18TAAGCCGTCTGCCAC CCGCCAAT TCAGCCTACG TGTCCACCCG archaea_19ACCCCTTGTGGTGCTCC sphingo_2_19 CACTGACTTACTTGT alpha_8_19ACCCGAGCAAGCTCG CCCGCCAA CAGCCTACGC GGTTTCTCGT archaea_20CCCTTGTGGTGCTCCCC sphingo_2_20 GACTTACTTGTCAGC alpha_8_20CCGTCTGCCACTGTCC CGCCAATT CTACGCACCC ACCCGAGCA archaea_21CACCCCTTGTGGTGCTC sphingo_2_21 TGACTTACTTGTCAG alpha_8_21AACCTCTAGGTAGAT CCCCGCCA CCTACGCACC ACCCACGCGT archaea_22GTGTGTGCAAGGAGCA sphingo_2_22 CTGACTTACTTGTCA alpha_8_22GCCGTCTGCCACTGTC GGGACGTAT GCCTACGCAC CACCCGAGC archaea_23TGTGTGCAAGGAGCAG sphingo_2_23 ACTGACTTACTTGTC alpha_8_23 TAGATACCCACGCGTGGACGTATT AGCCTACGCA TACTAAGCCG archaea_24 CGGTGTGTGCAAGGAG sphingo_2_24CCATGCAGCACCTC alpha_8_24 AAGCCGTCTGCCACT CAGGGACGT GTATAGAGTCCGTCCACCCGA archaea_25 GGTGTGTGCAAGGAGC sphingo_2_25 CGCTTTCGCTTAGCCalpha_8_25 GTAGATACCCACGCG AGGGACGTA ACTTACTGTA TTACTAAGCC bacteria_1CGCTCGTTGCGGGACTT sphingo_3_1 AGTTTCCTCGAGCTA alpha_9_1 TCTCCGGCGACCAAAAACCCAAC TGCCCCAGTT CTCCCCATGT bacteria_2 GCTCGTTGCGGGACTTA sphingo_3_2CGAGTTTCCTCGAG alpha_9_2 CGTCTCCGGCGACCA ACCCAACA CTATGCCCCAG AACTCCCCATbacteria_3 GACTTAACCCAACATCT sphingo_3_3 GTTTCCTCGAGCTAT alpha_9_3GTCTCCGGCGACCAA CACGACAC GCCCCAGTTA ACTCCCCATG bacteria_4AACCCAACATCTCACGA sphingo_3_4 TTTCCTCGAGCTATG alpha_9_4 CTCCGGCGACCAAACCACGAGCT CCCCAGTTAA TCCCCATGTC bacteria_5 ACTTAACCCAACATCTC sphingo_3_5GAGTTTCCTCGAGCT alpha_9_5 GCCGTCTCCGGCGAC ACGACACG ATGCCCCAGT CAAACTCCCCbacteria_6 TAACCCAACATCTCACG sphingo_3_6 TCGAGTTTCCTCGAG alpha_9_6TCCGGCGACCAAACT ACACGAGC CTATGCCCCA CCCCATGTCA bacteria_7GGACTTAACCCAACATC sphingo_3_7 TTACCGAAGTAAAT alpha_9_7 CCGTCTCCGGCGACCTCACGACA GCTGCCCCTCG AAACTCCCCA bacteria_8 CTTAACCCAACATCTCA sphingo_3_8GTTGCTAGCTCTACC alpha_9_8 CGCCGTCTCCGGCGA CGACACGA CTAAACAGCG CCAAACTCCCbacteria_9 TTAACCCAACATCTCAC sphingo_3_9 AGTTGCTAGCTCTAC alpha_9_9CCGGCGACCAAACTC GACACGAG CCTAAACAGC CCCATGTCAA bacteria_10GGGACTTAACCCAACAT sphingo_3_10 CCATTTACCGAAGT alpha_9_10 ACGCCGTCTCCGGCGCTCACGAC AAATGCTGCCC ACCAAACTCC bacteria_11 ACTGCTGCCTCCCGTAGsphingo_3_11 CATTTACCGAAGTA alpha_9_11 GAACTGAAGGACGCC GAGTCTGGAATGCTGCCCC GTCTCCGGCG bacteria_12 CTCGTTGCGGGACTTAA sphingo_3_12CGCCATTTACCGAA alpha_9_12 CGGCGACCAAACTCC CCCAACAT GTAAATGCTGCCCATGTCAAG bacteria_13 CGGGACTTAACCCAACA sphingo_3_13 TTGCTAGCTCTACCCalpha_9_13 GTCGGCAGCCTCCCTT TCTCACGA TAAACAGCGC ACGGGTCGG bacteria_14TCGTTGCGGGACTTAAC sphingo_3_14 GCCATTTACCGAAG alpha_9_14 GGTCGGCAGCCTCCCCCAACATC TAAATGCTGCC TTACGGGTCG bacteria_15 CGTTGCGGGACTTAACCsphingo_3_15 TCCTCGAGCTATGCC alpha_9_15 TGGTCGGCAGCCTCC CAACATCTCCAGTTAAAG CTTACGGGTC bacteria_16 GTTGCGGGACTTAACCC sphingo_3_16TTCCTCGAGCTATGC alpha_9_16 TCGGCAGCCTCCCTTA AACATCTC CCCAGTTAAACGGGTCGGC bacteria_17 TGCGGGACTTAACCCAA sphingo_3_17 CAGTTGCTAGCTCTAalpha_9_17 GTGGTCGGCAGCCTC CATCTCAC CCCTAAACAG CCTTACGGGT bacteria_18TTGCGGGACTTAACCCA sphingo_3_18 TGCTAGCTCTACCCT alpha_9_18CGTGGTCGGCAGCCT ACATCTCA AAACAGCGCC CCCTTACGGG bacteria_19CCCCACTGCTGCCTCCC sphingo_3_19 CCGTCAGATCCTCTC alpha_9_19CGGCAGCCTCCCTTA GTAGGAGT GCAAGAGTAT CGGGTCGGCG bacteria_20GCGGGACTTAACCCAAC sphingo_3_20 CTCGAGCTATGCCC alpha_9_20 CGCACCTCAGCGTCAATCTCACG CAGTTAAAGGT GATCCGGACC bacteria_21 GCGCTCGTTGCGGGACTsphingo_3_21 CCTCGAGCTATGCC alpha_9_21 AATCTTTCCCCCTCAG TAACCCAACCAGTTAAAGG GGCTTATCC bacteria_22 TCCCCACTGCTGCCTCC sphingo_3_22CCAGTTGCTAGCTCT alpha_9_22 CGAACTGAAGGACGC CGTAGGAG ACCCTAAACACGTCTCCGGC bacteria_23 ATTCCCCACTGCTGCCT sphingo_3_23 TCTCTCTGGATGTCAalpha_9_23 TACCCTCTTCCGATCT CCCGTAGG CTCGCATTCT CTAGCCTAG bacteria_24TTCCCCACTGCTGCCTC sphingo_3_24 ATCTCTCTGGATGTC alpha_9_24GGCAGCCTCCCTTAC CCGTAGGA ACTCGCATTC GGGTCGGCGA bacteria_25ACCCAACATCTCACGAC sphingo_3_25 CTCTCTGGATGTCAC alpha_9_25GGCGACCAAACTCCC ACGAGCTG TCGCATTCTA CATGTCAAGG rhodobacter_1TCCCCAGGCGGAATGCT caldithrix_1_1 ACTCCTCAGAGCTTC alpha_10_1CGCACCTGAGCGTCA TAATCCGT ATCGCCCACG GATCTAGTCC rhodobacter_2CTCCCCAGGCGGAATGC caldithrix_1_2 CTCCTCAGAGCTTCA alpha_10_2TCGCACCTGAGCGTC TTAATCCG TCGCCCACGC AGATCTAGTC rhodobacter_3ACTCCCCAGGCGGAATG caldithrix_1_3 AACAGGGCTTTACA alpha_10_3CGTGCGCCACTCTCC CTTAATCC CTCCTCAGAGC AGTTCCCGAA rhodobacter_4CCCCAGGCGGAATGCTT caldithrix_1_4 CACTCCTCAGAGCTT alpha_10_4CCGTGCGCCACTCTCC AATCCGTT CATCGCCCAC AGTTCCCGA rhodobacter_5CACCGCGTCATGCTGTT caldithrix_1_5 ACAGGGCTTTACAC alpha_10_5CCCGTGCGCCACTCTC ACGCGATT TCCTCAGAGCT CAGTTCCCG rhodobacter_6TCACCGCGTCATGCTGT caldithrix_1_6 ACACTCCTCAGAGC alpha_10_6CTGAGCGTCAGATCT TACGCGAT TTCATCGCCCA AGTCCAGGTG rhodobacter_7ATTCACCGCGTCATGCT caldithrix_1_7 CAGGGCTTTACACT alpha_10_7TTCGCACCTGAGCGT GTTACGCG CCTCAGAGCTT CAGATCTAGT rhodobacter_8TAGCCCAACCCGTAAGG caldithrix_1_8 TCCTCAGAGCTTCAT alpha_10_8CCAACCGTTATCCCCC GCCATGAG CGCCCACGCG ACTAAGAGG rhodobacter_9TACTCCCCAGGCGGAAT caldithrix_1_9 TACACTCCTCAGAG alpha_10_9TCCAACCGTTATCCCC GCTTAATC CTTCATCGCCC CACTAAGAG rhodobacter_10AGCCCAACCCGTAAGG caldithrix_1_10 CTTCTGGCACTCCCG alpha_10_10GCACCTGAGCGTCAG GCCATGAGG ACTTTCATGG ATCTAGTCCA rhodobacter_11GCCCAACCCGTAAGGG caldithrix_1_11 TTACACTCCTCAGA alpha_10_11CCTGAGCGTCAGATC CCATGAGGA GCTTCATCGCC TAGTCCAGGT rhodobacter_12AACGTATTCACCGCGTC caldithrix_1_12 CCTCAGAGCTTCATC alpha_10_12GTTAGCCCACCGTCTT ATGCTGTT GCCCACGCGG CGGGTAAAA rhodobacter_13TTCACCGCGTCATGCTG caldithrix_1_13 CCTAACAGGGCTTT alpha_10_13CCACTAAGAGGTAGG TTACGCGA ACACTCCTCAG TCCCCACGCG rhodobacter_14ACCGCGTCATGCTGTTA caldithrix_1_14 AGGGCTTTACACTC alpha_10_14TGAGCGTCAGATCTA CGCGATTA CTCAGAGCTTC GTCCAGGTGG rhodobacter_15GCGGAATGCTTAATCCG caldithrix_1_15 TTCTGGCACTCCCGA alpha_10_15ATCCCCCACTAAGAG TTAGGTGT CTTTCATGGC GTAGGTCCCC rhodobacter_16CCAACCCGTAAGGGCC caldithrix_1_16 TCTGGCACTCCCGA alpha_10_16GCTTTCACCCCTGACT ATGAGGACT CTTTCATGGCG GGCAAGACC rhodobacter_17CCCAGGCGGAATGCTTA caldithrix_1_17 CTCAGAGCTTCATC alpha_10_17CAACCGTTATCCCCC ATCCGTTA GCCCACGCGGC ACTAAGAGGT rhodobacter_18CCCAACCCGTAAGGGCC caldithrix_1_18 GGGCTTTACACTCCT alpha_10_18GCGTCACCGAAATCG ATGAGGAC CAGAGCTTCA AAATCCCGAC rhodobacter_19AATTCCACTCACCTCTC caldithrix_1_19 CTCCTAACAGGGCT alpha_10_19TGCGTCACCGAAATC TCGAACTC TTACACTCCTC GAAATCCCGA rhodobacter_20GAATTCCACTCACCTCT caldithrix_1_20 CTGGCACTCCCGAC alpha_10_20CGTCACCGAAATCGA CTCGAACT TTTCATGGCGT AATCCCGACA rhodobacter_21TATTCACCGCGTCATGC caldithrix_1_21 TCAGAGCTTCATCG alpha_10_21CTGCGTCACCGAAAT TGTTACGC CCCACGCGGCG CGAAATCCCG rhodobacter_22ACGTATTCACCGCGTCA caldithrix_1_22 ACCTCTACAGCAGT alpha_10_22TTTCGCACCTGAGCGT TGCTGTTA CCCGAAGGAAG CAGATCTAG rhodobacter_23GAACGTATTCACCGCGT caldithrix_1_23 CCCTCCTAACAGGG alpha_10_23CTTTCACCCCTGACTG CATGCTGT TTTTACACTCC GCAAGACCG rhodobacter_24GGAATTCCACTCACCTC caldithrix_1_24 GGTCGAAACCTCCA alpha_10_24CTAAAAGGTTAGCCC TCTCGAAC ACACCTAGTGC ACCGTCTTCG rhodobacter_25GTAGCCCAACCCGTAAG caldithrix_1_25 GTCGAAACCTCCAA alpha_10_25CCCACTAAGAGGTAG GGCCATGA CACCTAGTGCC GTCCCCACGC margrpA_1ACGAAGTTAGCCGGTGC chloroflexi_1_1 TCTCCGAGGAGTCG alpha_12_1CCGTGCGCCACTCTAT TTTCTTGT TTCCAGTTTCC AAATAGCGT margrpA_2CACGAAGTTAGCCGGTG chloroflexi_1_2 CTCCGAGGAGTCGT alpha_12_2CCCGTGCGCCACTCT CTTTCTTG TCCAGTTTCCC ATAAATAGCG margrpA_3GTTACTCACCCGTTCGC chloroflexi_1_3 ACGAATGGGTTTGA alpha_12_3CCAACCGTTATCCCG CAGTTTAC CACCACCCACA CAGAAAAAGG margrpA_4TAAGGGACATACTGACT chloroflexi_1_4 CGAATGGGTTTGAC alpha_12_4CCCGCAGAAAAAGGC TGACATCA ACCACCCACAC AGGTTCCCAC margrpA_5ATAAGGGACATACTGA chloroflexi_1_5 CTCTCCGAGGAGTC alpha_12_5ACCGTTATCCCGCAG CTTGACATC GTTCCAGTTTC AAAAAGGCAG margrpA_6AAGGGACATACTGACTT chloroflexi_1_6 TCCGAGGAGTCGTT alpha_12_6CAACCGTTATCCCGC GACATCAT CCAGTTTCCCT AGAAAAAGGC margrpA_7TTACTCACCCGTTCGCC chloroflexi_1_7 GAATGGGTTTGACA alpha_12_7CGTTTCCAACCGTTAT AGTTTACT CCACCCACACC CCCGCAGAA margrpA_8CGTTACTCACCCGTTCG chloroflexi_1_8 GCTCTCCGAGGAGT alpha_12_8CCGCAGAAAAAGGCA CCAGTTTA CGTTCCAGTTT GGTTCCCACG margrpA_9GCGTTACTCACCCGTTC chloroflexi_1_9 CCGAGGAGTCGTTC alpha_12_9CGCAGAAAAAGGCAG GCCAGTTT CAGTTTCCCTT GTTCCCACGC margrpA_10CGCGTTACTCACCCGTT chloroflexi_1_10 CGCTCTCCGAGGAG alpha_12_10CCGTTATCCCGCAGA CGCCAGTT TCGTTCCAGTT AAAAGGCAGG margrpA_11ACATACTGACTTGACAT chloroflexi_1_11 AATGGGTTTGACAC alpha_12_11CGTTATCCCGCAGAA CATCCCCA CACCCACACCT AAAGGCAGGT margrpA_12TACTGACTTGACATCAT chloroflexi_1_12 CGAGGAGTCGTTCC alpha_12_12ACCCGTGCGCCACTC CCCCACCT AGTTTCCCTTC TATAAATAGC margrpA_13GGACATACTGACTTGAC chloroflexi_1_13 AGGAGTCGTTCCAG alpha_12_13CACCCGTGCGCCACT ATCATCCC TTTCCCTTCAC CTATAAATAG margrpA_14GACATACTGACTTGACA chloroflexi_1_14 GAGGAGTCGTTCCA alpha_12_14TCCCGCAGAAAAAGG TCATCCCC GTTTCCCTTCA CAGGTTCCCA margrpA_15ATACTGACTTGACATCA chloroflexi_1_15 CGCTTTGCGACATG alpha_12_15GCAGAAAAAGGCAGG TCCCCACC AGCGTCAGGTT TTCCCACGCG margrpA_16CATACTGACTTGACATC chloroflexi_1_16 TGAGCGTCAGGTTC alpha_12_16GGAAACCAAACTCCC ATCCCCAC AATGCCAGGGT CATGTCAAGG margrpA_17AGGGACATACTGACTTG chloroflexi_1_17 ACGCTTTGCGACAT alpha_12_17CCTCCTGCAAGCAGG ACATCATC GAGCGTCAGGT TTAGCTCACC margrpA_18GGGACATACTGACTTGA chloroflexi_1_18 TCCCCACGCTTTGCG alpha_12_18TTTCGCGCCTCAGCGT CATCATCC ACATGAGCGT CAAAATCGG margrpA_19ACGCGTTACTCACCCGT chloroflexi_1_19 TCAGGTTCAATGCC alpha_12_19TTCGCGCCTCAGCGTC TCGCCAGT AGGGTACCGCT AAAATCGGA margrpA_20GCACGAAGTTAGCCGGT chloroflexi_1_20 ATCATCTCGGCCTTC alpha_12_20ACTCCCCATGTCAAG GCTTTCTT ACGTTCGACT GACTGGTAAG margrpA_21GGCACGAAGTTAGCCG chloroflexi_1_21 TGCGACATGAGCGT alpha_12_21GCCTCCTGCAAGCAG GTGCTTTCT CAGGTTCAATG GTTAGCTCAC margrpA_22TGGCACGAAGTTAGCCG chloroflexi_1_22 ATGAGCGTCAGGTT alpha_12_22CAGAAAAAGGCAGGT GTGCTTTC CAATGCCAGGG TCCCACGCGT margrpA_23ACTGACTTGACATCATC chloroflexi_1_23 CACGCTTTGCGACA alpha_12_23TCCGGCGGACCTTTCC CCCACCTT TGAGCGTCAGG CCCGTAGGG margrpA_24CTGGCACGAAGTTAGCC chloroflexi_1_24 CATGAGCGTCAGGT alpha_12_24TATCCCGCAGAAAAA GGTGCTTT TCAATGCCAGG GGCAGGTTCC margrpA_25ACGATTACTAGCGATTC chloroflexi_1_25 GTAATCATCTCGGC alpha_12_25CCCCTCTTTCTCCGGC CTGCTTCA CTTCACGTTCG GGACCTTTC vibrionaceae_1TATCCCCCACATCAGGG chloroflexi_2_1 GGTGACTCCCCTTTC alpha_13_1TCTAACTGTTCAAGC CAATTTCC AGGTTGCTAC AGCCTGCGAG vibrionaceae_2CGACATTACTCGCTGGC chloroflexi_2_2 AGGTGACTCCCCTTT alpha_13_2CTAACTGTTCAAGCA AAACAAGG CAGGTTGCTA GCCTGCGAGC vibrionaceae_3CCGACATTACTCGCTGG chloroflexi_2_3 CCCTCCCCATTAAGC alpha_13_3TAACTGTTCAAGCAG CAAACAAG GGGGAGATTT CCTGCGAGCC vibrionaceae_4CCCCACATCAGGGCAAT chloroflexi_2_4 GCAAGCTTGGCTCA alpha_13_4GTCTAACTGTTCAAG TTCCTAGG TCGGTACCGTT CAGCCTGCGA vibrionaceae_5CCCCCACATCAGGGCAA chloroflexi_2_5 CTCTCCCGATGTTCC alpha_13_5CGCTCCTCAGCGTCA TTTCCTAG AAGCAAGCTT GAAAATAGCC vibrionaceae_6CCCACATCAGGGCAATT chloroflexi_2_6 CCCCTCCCCATTAAG alpha_13_6GCTCCTCAGCGTCAG TCCTAGGC CGGGGAGATT AAAATAGCCA vibrionaceae_7CCACATCAGGGCAATTT chloroflexi_2_7 TTCCAAGCAAGCTT alpha_13_7TCGCTCCTCAGCGTCA CCTAGGCA GGCTCATCGGT GAAAATAGC vibrionaceae_8TCCCCCACATCAGGGCA chloroflexi_2_8 AGCAAGCTTGGCTC alpha_13_8CGTCTAACTGTTCAA ATTTCCTA ATCGGTACCGT GCAGCCTGCG vibrionaceae_9CCCGACATTACTCGCTG chloroflexi_2_9 ACTCTCCCGATGTTC alpha_13_9AACTGTTCAAGCAGC GCAAACAA CAAGCAAGCT CTGCGAGCCC vibrionaceae_10ATCCCCCACATCAGGGC chloroflexi_2_10 ACCCCTCCCCATTAA alpha_13_10CACGTCTAACTGTTCA AATTTCCT GCGGGGAGAT AGCAGCCTG vibrionaceae_11TGGTTATCCCCCACATC chloroflexi_2_11 TCTCCCGATGTTCCA alpha_13_11ACGTCTAACTGTTCA AGGGCAAT AGCAAGCTTG AGCAGCCTGC vibrionaceae_12CCCCCACATCAGGGCAA chloroflexi_2_12 CTCCCGATGTTCCAA alpha_13_12ACTGTTCAAGCAGCC TTTCCCAG GCAAGCTTGG TGCGAGCCCT vibrionaceae_13TCCCCCACATCAGGGCA chloroflexi_2_13 AATGACCCCTCCCC alpha_13_13CCGGGGATTTCACGT ATTTCCCA ATTAAGCGGGG CTAACTGTTC vibrionaceae_14CCCCACATCAGGGCAAT chloroflexi_2_14 GAATGACCCCTCCC alpha_13_14CTCCTCAGCGTCAGA TTCCCAGG CATTAAGCGGG AAATAGCCAG vibrionaceae_15CCCACATCAGGGCAATT chloroflexi_2_15 GTTCCAAGCAAGCT alpha_13_15TTCAAGCAGCCTGCG TCCCAGGC TGGCTCATCGG AGCCCTTTAC vibrionaceae_16CACATCAGGGCAATTTC chloroflexi_2_16 CGAATGACCCCTCC alpha_13_16TGTTCAAGCAGCCTG CCAGGCAT CCATTAAGCGG CGAGCCCTTT vibrionaceae_17CCACATCAGGGCAATTT chloroflexi_2_17 TGTTCCAAGCAAGC alpha_13_17CTGTTCAAGCAGCCT CCCAGGCA TTGGCTCATCG GCGAGCCCTT vibrionaceae_18ATCCCCCACATCAGGGC chloroflexi_2_18 TCGAATGACCCCTC alpha_13_18GTTCAAGCAGCCTGC AATTTCCC CCCATTAAGCG GAGCCCTTTA vibrionaceae_19TCCCGACATTACTCGCT chloroflexi_2_19 AAGCAAGCTTGGCT alpha_13_19CGGCATTGCTGGATC GGCAAACA CATCGGTACCG AGAGTTGCCT vibrionaceae_20GGTTATCCCCCACATCA chloroflexi_2_20 TGACCCCTCCCCATT alpha_13_20GGCATTGCTGGATCA GGGCAATT AAGCGGGGAG GAGTTGCCTC vibrionaceae_21CGCAAGTTGGCCGCCCT chloroflexi_2_21 CCACTCTCCCGATGT alpha_13_21CGCGGCATTGCTGGA CTGTATGC TCCAAGCAAG TCAGAGTTGC vibrionaceae_22GCAAGTTGGCCGCCCTC chloroflexi_2_22 CCTCCCCATTAAGC alpha_13_22GCATTGCTGGATCAG TGTATGCG GGGGAGATTTC AGTTGCCTCC vibrionaceae_23ATGGTTATCCCCCACAT chloroflexi_2_23 CAAGCTTGGCTCAT alpha_13_23GCGGCATTGCTGGAT CAGGGCAA CGGTACCGTTC CAGAGTTGCC vibrionaceae_24ACTCGCTGGCAAACAA chloroflexi_2_24 CCGATGTTCCAAGC alpha_13_24CCCGGGGATTTCACG GGATAAGGG AAGCTTGGCTC TCTAACTGTT vibrionaceae_25CGCATCTGAGTGTCAGT chloroflexi_2_25 CACTCTCCCGATGTT alpha_13_25ACGCGGCATTGCTGG ATCTGTCC CCAAGCAAGC ATCAGAGTTG alteromonadales_1CCCACTTGGGCCAATCT chlorella_p1_1 CGCCACTCATCGCA delta_1_1CCGAACTACGAACTG AAAGGCGA ATCTGGCAAGC CTTTCTGGGA alteromonadales_2ATCCCACTTGGGCCAAT chlorella_p1_2 GCCACTCATCGCAA delta_1_2TCCGAACTACGAACT CTAAAGGC TCTGGCAAGCC GCTTTCTGGG alteromonadales_3TCCCACTTGGGCCAATC chlorella_p1_3 CCACTCATCGCAAT delta_1_3TTGCTGCGGCACAGC TAAAGGCG CTGGCAAGCCA AGGGGTCAAT alteromonadales_4CCACTTGGGCCAATCTA chlorella_p1_4 CACTCATCGCAATCT delta_1_4GTTTGCTGCGGCACA AAGGCGAG GGCAAGCCAA GCAGGGGTCA alteromonadales_5CACTTGGGCCAATCTAA chlorella_p1_5 GCAAGCCAAATTGC delta_1_5TTTGCTGCGGCACAG AGGCGAGA ATGCGTACGAC CAGGGGTCAA alteromonadales_6ACTTGGGCCAATCTAAA chlorella_p1_6 GCCAAATTGCATGC delta_1_6TTGCCCAACGACTTCT GGCGAGAG GTACGACTTGC GGTACAACC alteromonadales_7CTTGGGCCAATCTAAAG chlorella_p1_7 TGGCAAGCCAAATT delta_1_7GGTTTGCCCAACGAC GCGAGAGC GCATGCGTACG TTCTGGTACA alteromonadales_8CACCTCAAGGCATGTTC chlorella_p1_8 CTGTGTCCACTCTGG delta_1_8TCCCCGAAGGGTTTG CCAAGCAT AACTTCCCCT CCCAACGACT alteromonadales_9TGAGCGTCAGTGTTGAC chlorella_p1_9 CCGTCCGCCACTCAT delta_1_9CCCCGAAGGGTTTGC CCAGGTGG CGCAATCTGG CCAACGACTT alteromonadales_10CGAAGCCCCCTTTGGTC chlorella_p1_10 CCGCCACTCATCGC delta_1_10CCGAAGGGTTTGCCC CGTAGACA AATCTGGCAAG AACGACTTCT alteromonadales_11ACAGAACCGAGGTTCC chlorella_p1_11 CGTCCGCCACTCATC delta_1_11CCCGAAGGGTTTGCC GAGCTTCTA GCAATCTGGC CAACGACTTC alteromonadales_12CAGAACCGAGGTTCCG chlorella_p1_12 CCTGTGTCCACTCTG delta_1_12CCCGGGCTTTCACAC AGCTTCTAG GAACTTCCCC CTGACTTAAA alteromonadales_13AGAACCGAGGTTCCGA chlorella_p1_13 GTCCGCCACTCATC delta_1_13GCTTCCTTCAGTGGTA GCTTCTAGT GCAATCTGGCA CCGTCAACA alteromonadales_14GAAAAACAGAACCGAG chlorella_p1_14 TCCGCCACTCATCGC delta_1_14AGGCGCCTGCATCCC GTTCCGAGC AATCTGGCAA CGAAGGGTTT alteromonadales_15GAACCGAGGTTCCGAG chlorella_p1_15 ACCTGTGTCCACTCT delta_1_15GGCGCCTGCATCCCC CTTCTAGTA GGAACTTCCC GAAGGGTTTG alteromonadales_16CCGAGGTTCCGAGCTTC chlorella_p1_16 GGCAAGCCAAATTG delta_1_16GCGCCTGCATCCCCG TAGTAGAC CATGCGTACGA AAGGGTTTGC alteromonadales_17CGAGGTTCCGAGCTTCT chlorella_p1_17 CTGGCAAGCCAAAT delta_1_17GCATCCCCGAAGGGT AGTAGACA TGCATGCGTAC TTGCCCAACG alteromonadales_18AACCGAGGTTCCGAGCT chlorella_p1_18 CCCGTCCGCCACTC delta_1_18ATCCCCGAAGGGTTT TCTAGTAG ATCGCAATCTG GCCCAACGAC alteromonadales_19ACCGAGGTTCCGAGCTT chlorella_p1_19 CACCTGTGTCCACTC delta_1_19CATCCCCGAAGGGTT CTAGTAGA TGGAACTTCC TGCCCAACGA alteromonadales_20AACAGAACCGAGGTTC chlorella_p1_20 ACCCGTCCGCCACT delta_1_20ACCTTAGGCGCCTGC CGAGCTTCT CATCGCAATCT ATCCCCGAAG alteromonadales_21AAACAGAACCGAGGTT chlorella_p1_21 CCACCTGTGTCCACT delta_1_21CCTTAGGCGCCTGCA CCGAGCTTC CTGGAACTTC TCCCCGAAGG alteromonadales_22CCGAAGCCCCCTTTGGT chlorella_p1_22 CACCCGTCCGCCAC delta_1_22TACCTTAGGCGCCTG CCGTAGAC TCATCGCAATC CATCCCCGAA alteromonadales_23GAAGCCCCCTTTGGTCC chlorella_p1_23 TCACCCGTCCGCCA delta_1_23ATACCTTAGGCGCCT GTAGACAT CTCATCGCAAT GCATCCCCGA alteromonadales_24AAGCCCCCTTTGGTCCG chlorella_p1_24 ACCACCTGTGTCCA delta_1_24CTTAGGCGCCTGCAT TAGACATT CTCTGGAACTT CCCCGAAGGG alteromonadales_25CCACCTCAAGGCATGTT chlorella_p1_25 CACCACCTGTGTCC delta_1_25CATACCTTAGGCGCC CCCAAGCA ACTCTGGAACT TGCATCCCCG polaribacters_1GCCAGATGGCTGCTCAT plastid_1_1 GGTCTCACGACTTG delta_2_1 CTCCAGTCTTTCGATATGTCCATA GCATCTCATTG GGATTCCCG polaribacters_2 TGCCAGATGGCTGCTCAplastid_1_2 TCTCCCTAGGCAGG delta_2_2 GGCCACCCTTGATCC TTGTCCATTTTTTGACCTG AAAAACCCGA polaribacters_3 TTGCCAGATGGCTGCTC plastid_1_3CCACGTGGATTCGA delta_2_3 AGGCCACCCTTGATC ATTGTCCA TACACGCAATG CAAAAACCCGpolaribacters_4 CCAGATGGCTGCTCATT plastid_1_4 ATGCACCACCTGTA delta_2_4AAGGGCACTCCAGTC GTCCATAC TGTGTCTGCCG TTTCGATAGG polaribacters_5GTTGCCAGATGGCTGCT plastid_1_5 CACCACCTGTATGT delta_2_5 GAGGCCACCCTTGATCATTGTCC GTCTGCCGAAG CCAAAAACCC polaribacters_6 TCCCTCAGCGTCAGTACplastid_1_6 AACACCACGTGGAT delta_2_6 GAAGGGCACTCCAGT ATACGTAGTCGATACACGC CTTTCGATAG polaribacters_7 CCCTCAGCGTCAGTACA plastid_1_7ACCACCTGTATGTGT delta_2_7 ACCCTAGCAAGCTAG TACGTAGT CTGCCGAAGC AGTGTTCTCGpolaribacters_8 GTCCCTCAGCGTCAGTA plastid_1_8 CTTCTCCCTAGGCAG delta_2_8CATGTAGAGGCCACC CATACGTA GTTTTTGACC CTTGATCCAA polaribacters_9CAGATGGCTGCTCATTG plastid_1_9 TGCACCACCTGTAT delta_2_9 AGAGGCCACCCTTGATCCATACC GTGTCTGCCGA TCCAAAAACC polaribacters_10 TTCGCATAGTGGCTGCTplastid_1_10 ACACCACGTGGATT delta_2_10 ACATGTAGAGGCCAC CATTGTCCCGATACACGCA CCTTGATCCA polaribacters_11 CGTCCCTCAGCGTCAGT plastid_1_11CCACCTGTATGTGTC delta_2_11 TACATGTAGAGGCCA ACATACGT TGCCGAAGCACCCTTGATCC polaribacters_12 AGACCCCCTACCTATCG plastid_1_12GCACCACCTGTATG delta_2_12 CCCCGAAGGGCACTC TTGCCATG TGTCTGCCGAACAGTCTTTCG polaribacters_13 CGCTTAGTCACTGAGCT plastid_1_13CACCACGTGGATTC delta_2_13 CCCTAGCAAGCTAGA AATGCCCA GATACACGCAAGTGTTCTCGT polaribacters_14 TGTTGCCAGATGGCTGC plastid_1_14CTCACGACTTGGCA delta_2_14 GCTTACATGTAGAGG TCATTGTC TCTCATTGTCCCCACCCTTGA polaribacters_15 GATTCGCTCCTATTCGC plastid_1_15CAGGTACACGTCAG delta_2_15 GGGCACTCCAGTCTTT ATAGTGGC AAACTTCCTCCCGATAGGAT polaribacters_16 TCGTCCCTCAGCGTCAG plastid_1_16 CTCCCTAGGCAGGTdelta_2_16 CCGAAGGGCACTCCA TACATACG TTTTGACCTGT GTCTTTCGATpolaribacters_17 TCGCTTAGTCACTGAGC plastid_1_17 CGGTCTCACGACTTdelta_2_17 CGAAGGGCACTCCAG TAATGCCC GGCATCTCATT TCTTTCGATApolaribacters_18 TCGCATAGTGGCTGCTC plastid_1_18 GACCAACTACTGATdelta_2_18 AGGGCACTCCAGTCT ATTGTCCA CGTCACCTTGG TTCGATAGGApolaribacters_19 CAGACCCCCTACCTATC plastid_1_19 GCTTCTCCCTAGGCAdelta_2_19 CCCGAAGGGCACTCC GTTGCCAT GGTTTTTGAC AGTCTTTCGApolaribacters_20 TTCGTCCCTCAGCGTCA plastid_1_20 CACCTGTATGTGTCTdelta_2_20 CCAGTCTTTCGATAG GTACATAC GCCGAAGCAC GATTCCCGGGpolaribacters_21 CTCTCTGTTGCCAGATG plastid_1_21 CTGTATGTGTCTGCCdelta_2_21 TCCAGTCTTTCGATAG GCTGCTCA GAAGCACTTC GATTCCCGGpolaribacters_22 GCAGATTCTATACGCGT plastid_1_22 CATGCACCACCTGTdelta_2_22 GTCTTTCGATAGGATT TACGCACC ATGTGTCTGCC CCCGGGATGpolaribacters_23 GGCAGATTCTATACGCG plastid_1_23 AGGTACACGTCAGAdelta_2_23 CTTTCGATAGGATTCC TTACGCAC AACTTCCTCCC CGGGATGTCpolaribacters_24 CACCTCTGACTTAATTG plastid_1_24 TCGGTCTCACGACTTdelta_2_24 CAGTCTTTCGATAGG ACCGCCTG GGCATCTCAT ATTCCCGGGApolaribacters_25 CCTCTGACTTAATTGAC plastid_1_25 CCTTCTACTTCGACTdelta_2_25 GGGCTCCCCGAAGGG CGCCTGCG CTACTCGAGC CACTCCAGTCdesulfovibrionales_1 CCCGAGCATGCTGATCT plastid_2_1 CAGGTAACGTCAGAdelta_3_1 GGCACAGAAAGGGTC CGAATTAC ACTTCCTCCCT AACACTTCCTdesulfovibrionales_2 CACCCGAGCATGCTGAT plastid_2_2 AGGTAACGTCAGAAdelta_3_2 TCGGCACAGAAAGGG CTCGAATT CTTCCTCCCTG TCAACACTTCdesulfovibrionales_3 TCACCCGAGCATGCTGA plastid_2_3 GGTAACGTCAGAACdelta_3_3 CGGCACAGAAAGGGT TCTCGAAT TTCCTCCCTGA CAACACTTCCdesulfovibrionales_4 TTCACCCGAGCATGCTG plastid_2_4 TCAGGTAACGTCAGdelta_3_4 CTTCGGCACAGAAAG ATCTCGAA AACTTCCTCCC GGTCAACACTdesulfovibrionales_5 GCACCCTCTAATTTCCT plastid_2_5 CGCGTTAGCTATAATdelta_3_5 CACTTTACTCTCCCGA AGAGGTCC ACCGCATGGG CGAATCGGAdesulfovibrionales_6 AGGGCACCCTCTAATTT plastid_2_6 AATACCGCATGGGTdelta_3_6 CCACTTTACTCTCCCG CCTAGAGG CGATACATGCG ACGAATCGGdesulfovibrionales_7 GGGCACCCTCTAATTTC plastid_2_7 CTGTATGTACGTTCCdelta_3_7 GCTTCGGCACAGAAA CTAGAGGT CGAAGGTGGT GGGTCAACACdesulfovibrionales_8 CCCTCTAATTTCCTAGA plastid_2_8 CCTGTATGTACGTTCdelta_3_8 CTCTCCCGACGAATC GGTCCCCT CCGAAGGTGG GGAATTTCTCdesulfovibrionales_9 ACCCTCTAATTTCCTAG plastid_2_9 TCAGCCGCGAGCTCdelta_3_9 CCGACGAATCGGAAT AGGTCCCC CTCTCTAGGCA TTCTCGTTCGdesulfovibrionales_10 ATTTCCTAGAGGTCCCC plastid_2_10 ATACCGCATGGGTCdelta_3_10 GCCACTTTACTCTCCC TGGATGTC GATACATGCGA GACGAATCGdesulfovibrionales_11 AGGGTACCGTCAAATGC plastid_2_11 ACCTGTATGTACGTTdelta_3_11 AGCTTCGGCACAGAA CTACCCTA CCCGAAGGTG AGGGTCAACAdesulfovibrionales_12 GAGGGTACCGTCAAAT plastid_2_12 GCCGCGAGCTCCTCdelta_3_12 ACTCTCACGAGTTCG GCCTACCCT TCTAGGCAGAA CTACCCTTTGdesulfovibrionales_13 GGGTACCGTCAAATGCC plastid_2_13 GCGCCTTCCTCCAAdelta_3_13 TCTCCCGACGAATCG TACCCTAT ACGGTTAGAAT GAATTTCTCGdesulfovibrionales_14 TTTCCTAGAGGTCCCCT plastid_2_14 AGCCGCGAGCTCCTdelta_3_14 TAGCTTCGGCACAGA GGATGTCA CTCTAGGCAGA AAGGGTCAACdesulfovibrionales_15 TTCCTAGAGGTCCCCTG plastid_2_15 CAGCCGCGAGCTCCdelta_3_15 CTCTCACGAGTTCGCT GATGTCAA TCTCTAGGCAG ACCCTTTGTdesulfovibrionales_16 TGAGGGTACCGTCAAAT plastid_2_16 CACCTGTATGTACGTdelta_3_16 GTGCTGGTTACACCC GCCTACCC TCCCGAAGGT GAAGGCAATCdesulfovibrionales_17 CTCTAATTTCCTAGAGG plastid_2_17 AATCAGCCGCGAGCdelta_3_17 CGCCACTTTACTCTCC TCCCCTGG TCCTCTCTAGG CGACGAATCdesulfovibrionales_18 CACCCTCTAATTTCCTA plastid_2_18 TAATCAGCCGCGAGdelta_3_18 CTCCCGACGAATCGG GAGGTCCC CTCCTCTCTAG AATTTCTCGTdesulfovibrionales_19 GGCACCCTCTAATTTCC plastid_2_19 ATCAGCCGCGAGCTdelta_3_19 CTTACTCTCACGAGTT TAGAGGTC CCTCTCTAGGC CGCTACCCTdesulfovibrionales_20 CCTCTAATTTCCTAGAG plastid_2_20 GGCGCCTTCCTCCAdelta_3_20 TGTGCTGGTTACACCC GTCCCCTG AACGGTTAGAA GAAGGCAATdesulfovibrionales_21 CAACCGTTATCCCCGTC plastid_2_21 CCGCGAGCTCCTCTCdelta_3_21 CTCACGAGTTCGCTA TTGAAGGT TAGGCAGAAA CCCTTTGTACdesulfovibrionales_22 ATCAAAGGCTGTTCCAC plastid_2_22 GCATGGGTCGATACdelta_3_22 CTGTGCTGGTTACACC CGTTGAGC ATGCGACATCT CGAAGGCAAdesulfovibrionales_23 TTGCTCGTTAGCTCGCC plastid_2_23 CCGCATGGGTCGATdelta_3_23 TCGCCACTTTACTCTC GGCTTCGG ACATGCGACAT CCGACGAATdesulfovibrionales_24 ATTGCTCGTTAGCTCGC plastid_2_24 TACCGCATGGGTCGdelta_3_24 CCTGTGCTGGTTACAC CGGCTTCG ATACATGCGAC CCGAAGGCAdesulfovibrionales_25 CCTAGAGGTCCCCTGGA plastid_2_25 ACCGCATGGGTCGAdelta_3_25 GCTTACTCTCACGAGT TGTCAAGC TACATGCGACA TCGCTACCC aquaficae_1AACCAGACGCTCCACCG plastid_3_1 CACCGTCGTATATCT altero_1_1 CCCACTTGGGCCAATGTTGTGCG GACCGACGAT CTAAAGGCGA aquaficae_2 ACCAGACGCTCCACCGG plastid_3_2TTCACCGTCGTATAT altero_1_2 ATCCCACTTGGGCCA TTGTGCGG CTGACCGACGATCTAAAGGC aquaficae_3 AAACCAGACGCTCCACC plastid_3_3 TCACCGTCGTATATCaltero_1_3 TCCCACTTGGGCCAA GGTTGTGC TGACCGACGA TCTAAAGGCG aquaficae_4TGCCACTGTAGCGCCTG plastid_3_4 GTAGCCGAGTTTCA altero_1_4 CCACTTGGGCCAATCTGTAGCCC GGCTACAATCC TAAAGGCGAG aquaficae_5 TAAACCAGACGCTCCACplastid_3_5 TAGCCGAGTTTCAG altero_1_5 CACTTGGGCCAATCT CGGTTGTGGCTACAATCCG AAAGGCGAGA aquaficae_6 GCCACTGTAGCGCCTGT plastid_3_6GACCTCATCCTCACC altero_1_6 ACTTGGGCCAATCTA GTAGCCCA TTCCTCCAATAAGGCGAGAG aquaficae_7 CCAGACGCTCCACCGGT plastid_3_7 AGCCGAGTTTCAGGaltero_1_7 CTTGGGCCAATCTAA TGTGCGGG CTACAATCCGA AGGCGAGAGC aquaficae_8CCACTGTAGCGCCTGTG plastid_3_8 GCCGAGTTTCAGGC altero_1_8 CTGTCAGTAACGTCATAGCCCAG TACAATCCGAA CAGCTAGCAG aquaficae_9 GCATAAAGGGCATACT plastid_3_9CCGAGTTTCAGGCT altero_1_9 ACAGAACCGAGGTTC GACCTGACG ACAATCCGAACCGAGCTTCTA aquaficae_10 TTAAACCAGACGCTCCA plastid_3_10 CTCCCGTAGGAGTCaltero_1_10 CAGAACCGAGGTTCC CCGGTTGT TGTTCCGTTCT GAGCTTCTAG aquaficae_11CATTGCCCACGATTCCC plastid_3_11 CCTCCCGTAGGAGT altero_1_11AGAACCGAGGTTCCG CACTGCTG CTGTTCCGTTC AGCTTCTAGT aquaficae_12ATTGCCCACGATTCCCC plastid_3_12 TCCCGTAGGAGTCT altero_1_12GAAAAACAGAACCGA ACTGCTGC GTTCCGTTCTA GGTTCCGAGC aquaficae_13CCATTGCCCACGATTCC plastid_3_13 CCCGTAGGAGTCTG altero_1_13GAACCGAGGTTCCGA CCACTGCT TTCCGTTCTAA GCTTCTAGTA aquaficae_14GCCCATTGCCCACGATT plastid_3_14 TGACCTCATCCTCAC altero_1_14CCGAGGTTCCGAGCT CCCCACTG CTTCCTCCAA TCTAGTAGAC aquaficae_15CCCATTGCCCACGATTC plastid_3_15 CTAAAGCATTCATC altero_1_15CGAGGTTCCGAGCTT CCCACTGC CTCCACGCGGT CTAGTAGACA aquaficae_16CGCCCATTGCCCACGAT plastid_3_16 CCTAAAGCATTCAT altero_1_16AACCGAGGTTCCGAG TCCCCACT CCTCCACGCGG CTTCTAGTAG aquaficae_17TGCCCACGATTCCCCAC plastid_3_17 CCCTAAAGCATTCA altero_1_17ACCGAGGTTCCGAGC TGCTGCCC TCCTCCACGCG TTCTAGTAGA aquaficae_18ATTAAACCAGACGCTCC plastid_3_18 ACCCTAAAGCATTC altero_1_18AACAGAACCGAGGTT ACCGGTTG ATCCTCCACGC CCGAGCTTCT aquaficae_19TTGCCCACGATTCCCCA plastid_3_19 ACATAAGGGGCATG altero_1_19AAACAGAACCGAGGT CTGCTGCC CTGACTTGACC TCCGAGCTTC aquaficae_20GCCCACGATTCCCCACT plastid_3_20 GTTCCGTTCTAAATC altero_1_20CCAACTGTTGTCCCCC GCTGCCCC CCAGTGTGGC ACCTCAAGG aquaficae_21CAGACGCTCCACCGGTT plastid_3_21 CATAAGGGGCATGC altero_1_21CCGGACTACGACGCA GTGCGGGC TGACTTGACCT CTTTAAGTGA aquaficae_22GGCATAAAGGGCATAC plastid_3_22 GCGGTATTGCTTGGT altero_1_22TGGGCCAATCTAAAG TGACCTGAC CAAGCTTTCG GCGAGAGCCG aquaficae_23GCAGTTCGGAATGCCTT plastid_3_23 CGGTATTGCTTGGTC altero_1_23GGGCCAATCTAAAGG GCCGAAGT AAGCTTTCGC CGAGAGCCGA aquaficae_24CAGTTCGGAATGCCTTG plastid_3_24 CACGCGGTATTGCTT altero_1_24TTGGGCCAATCTAAA CCGAAGTT GGTCAAGCTT GGCGAGAGCC aquaficae_25CGCAGTTCGGAATGCCT plastid_3_25 CATCCTCCACGCGG altero_1_25GGTTCCGAGCTTCTA TGCCGAAG TATTGCTTGGT GTAGACATCG bacilli_1CACTCTGCTCCCGAAGG plastid_4_1 CTTAAGCGCCGCCC altero_2_1 TCTCACTTGGGCCTCTAGAAGCCC TCCGAATGGTT CTTTGCGCC bacilli_2 GTCACTCTGCTCCCGAA plastid_4_2CCTTAAGCGCCGCC altero_2_2 CCCCTCGCAAAGGCA GGAGAAGC CTCCGAATGGTAGTTCCCAAG bacilli_3 CTGCTCCCGAAGGAGA plastid_4_3 TACCTTAAGCGCCGaltero_2_3 CCCTCGCAAAGGCAA AGCCCTATC CCCTCCGAATG GTTCCCAAGC bacilli_4TCACTCTGCTCCCGAAG plastid_4_4 ACCTTAAGCGCCGC altero_2_4 TCACTTGGGCCTCTCTGAGAAGCC CCTCCGAATGG TTGCGCCGG bacilli_5 TCTGCTCCCGAAGGAGA plastid_4_5AGCCCTACCTTAAG altero_2_5 CTTGGGCCTCTCTTTG AGCCCTAT CGCCGCCCTCCCGCCGGAGC bacilli_6 TGCTCCCGAAGGAGAA plastid_4_6 TTAAGCGCCGCCCTaltero_2_6 CGACATTCTTTAAGG GCCCTATCT CCGAATGGTTA GGTCCGCTCC bacilli_7CTCTGCTCCCGAAGGAG plastid_4_7 TAAGCGCCGCCCTC altero_2_7 CACTTGGGCCTCTCTTAAGCCCTA CGAATGGTTAG TGCGCCGGA bacilli_8 GCTCCCGAAGGAGAAG plastid_4_8TAGCCCTACCTTAA altero_2_8 CTCACTTGGGCCTCTC CCCTATCTC GCGCCGCCCTCTTTGCGCCG bacilli_9 ACTCTGCTCCCGAAGGA plastid_4_9 CTACCTTAAGCGCCaltero_2_9 ACTTGGGCCTCTCTTT GAAGCCCT GCCCTCCGAAT GCGCCGGAG bacilli_10CCGAAGCCGCCTTTCAA plastid_4_10 GCCCTACCTTAAGC altero_2_10CTACGACATTCTTTAA TTTCGAAC GCCGCCCTCCG GGGGTCCGC bacilli_11CGTCCGCCGCTAACTTC plastid_4_11 CCCTACCTTAAGCG altero_2_11CCGGACTACGACATT ATAAGAGC CCGCCCTCCGA CTTTAAGGGG bacilli_12GTCCGCCGCTAACTTCA plastid_4_12 CCTACCTTAAGCGC altero_2_12ATCTCACTTGGGCCTC TAAGAGCA CGCCCTCCGAA TCTTTGCGC bacilli_13CCGCCGCTAACTTCATA plastid_4_13 CTAGCCCTACCTTAA altero_2_13CCCCCTCGCAAAGGC AGAGCAAG GCGCCGCCCT AAGTTCCCAA bacilli_14AGCCGAAGCCGCCTTTC plastid_4_14 ACTAGCCCTACCTTA altero_2_14ACATTCTTTAAGGGG AATTTCGA AGCGCCGCCC TCCGCTCCAC bacilli_15CTCCCGAAGGAGAAGC plastid_4_15 AAGCGCCGCCCTCC altero_2_15TTGGGCCTCTCTTTGC CCTATCTCT GAATGGTTAGG GCCGGAGCC bacilli_16CAGCCGAAGCCGCCTTT plastid_4_16 CACTAGCCCTACCTT altero_2_16TCCCCCTCGCAAAGG CAATTTCG AAGCGCCGCC CAAGTTCCCA bacilli_17CTGTCACTCTGCTCCCG plastid_4_17 CGCCGCCCTCCGAA altero_2_17CCTCGCAAAGGCAAG AAGGAGAA TGGTTAGGCTA TTCCCAAGCA bacilli_18GCCGAAGCCGCCTTTCA plastid_4_18 GCGCCGCCCTCCGA altero_2_18GGGTCCGCTCCACAT ATTTCGAA ATGGTTAGGCT CACTGTCTCG bacilli_19CCCGTCCGCCGCTAACT plastid_4_19 GCCGCCCTCCGAAT altero_2_19ACGACATTCTTTAAG TCATAAGA GGTTAGGCTAA GGGTCCGCTC bacilli_20CCGTCCGCCGCTAACTT plastid_4_20 AGCGCCGCCCTCCG altero_2_20CATTCTTTAAGGGGTC CATAAGAG AATGGTTAGGC CGCTCCACA bacilli_21CGCCGCTAACTTCATAA plastid_4_21 ACGAGATTAGCTAG altero_2_21GACATTCTTTAAGGG GAGCAAGC CCTTCGCAGGT GTCCGCTCCA bacilli_22CCCGAAGGAGAAGCCC plastid_4_22 CCGCCCTCCGAATG altero_2_22AATCTCACTTGGGCCT TATCTCTAG GTTAGGCTAAC CTCTTTGCG bacilli_23CGAAGGAGAAGCCCTA plastid_4_23 CGCCCTCCGAATGG altero_2_23 TAAGGGGTCCGCTCCTCTCTAGGG TTAGGCTAACG ACATCACTGT bacilli_24 CCGAAGGAGAAGCCCTplastid_4_24 GCCCTCCGAATGGT altero_2_24 ATCCCCCTCGCAAAG ATCTCTAGGTAGGCTAACGA GCAAGTTCCC bacilli_25 TGTCACTCTGCTCCCGA plastid_4_25TCACTAGCCCTACCT altero_2_25 GGTCCGCTCCACATC AGGAGAAG TAAGCGCCGCACTGTCTCGC crenarch_1_1 AGCCTGTACGTTGAGCG plastid_5_1 CTCTACCCCTACCATcolwel_1_1 TGCGCCACTCACGGA TACAGATT ACTCAAGCCT TCAAGTCCAC crenarch_1_2CCTGTACGTTGAGCGTA plastid_5_2 GACGTCGTCCTCCA colwel_1_2 CTGCGCCACTCACGGCAGATTTA AATGGTTAGAC ATCAAGTCCA crenarch_1_3 GCCTGTACGTTGAGCGTplastid_5_3 CCTTAGACGTCGTCC colwel_1_3 GCTGCGCCACTCACG ACAGATTTTCCAAATGGT GATCAAGTCC crenarch_1_4 GAGCGTACAGATTTAAC plastid_5_4ACCTTAGACGTCGT colwel_1_4 TAGCTGCGCCACTCA CGAAAACT CCTCCAAATGGCGGATCAAGT crenarch_1_5 TGAGCGTACAGATTTAA plastid_5_5 CCTCTACCCCTACCAcolwel_1_5 GTTAGCTGCGCCACT CCGAAAAC TACTCAAGCC CACGGATCAA crenarch_1_6CAGCCTGTACGTTGAGC plastid_5_6 GCTAGTTCTCGCGA colwel_1_6 CGTTAGCTGCGCCACGTACAGAT ATTTGCGACTC TCACGGATCA crenarch_1_7 CCTTGTCACGAACCTCAplastid_5_7 CCTCTCGGCATATG colwel_1_7 GTGCGTTAGCTGCGC AGTTCGATGGGATTTAGCT CACTCACGGA crenarch_1_8 CTTGTCACGAACCTCAA plastid_5_8GACTAACGGTGTTG colwel_1_8 TGCGTTAGCTGCGCC GTTCGATA GGTATGACCAGACTCACGGAT crenarch_1_9 TTGTCACGAACCTCAAG plastid_5_9 ACTAACGGTGTTGGcolwel_1_9 TTAGCTGCGCCACTC TTCGATAA GTATGACCAGC ACGGATCAAG crenarch_1_10CTGTACGTTGAGCGTAC plastid_5_10 CCAACAGTTATTCCC colwel_1_10GCGTTAGCTGCGCCA AGATTTAA CTCCTAAGGG CTCACGGATC crenarch_1_11GTCACGAACCTCAAGTT plastid_5_11 CTCTCGGCATATGG colwel_1_11AGCTGCGCCACTCAC CGATAACG GGATTTAGCTG GGATCAAGTC crenarch_1_12TTCCCTTGTCACGAACC plastid_5_12 GCGCGAGCTCATCC colwel_1_12GCGGTATTGCTGCCCT TCAAGTTC TTAGGCAGTGT CTGTACCTG crenarch_1_13TCACGAACCTCAAGTTC plastid_5_13 CGCGAGCTCATCCTT colwel_1_13CGCGGTATTGCTGCC GATAACGC AGGCAGTGTA CTCTGTACCT crenarch_1_14TGTCACGAACCTCAAGT plastid_5_14 GCGAGCTCATCCTT colwel_1_14GGATCAAGTCCACGA TCGATAAC AGGCAGTGTAA ACGGCTAGTT crenarch_1_15CTGCAGCACTGCATTGG plastid_5_15 CACCTCTCGGCATAT colwel_1_15CGGATCAAGTCCACG CCACAAGC GGGGATTTAG AACGGCTAGT crenarch_1_16GCAGCCTGTACGTTGAG plastid_5_16 ACCTCTCGGCATAT colwel_1_16GCGCCACTCACGGAT CGTACAGA GGGGATTTAGC CAAGTCCACG crenarch_1_17CACGAACCTCAAGTTCG plastid_5_17 GCAGCCTACAATCC colwel_1_17ACGGATCAAGTCCAC ATAACGCC GAACTTGGACA GAACGGCTAG crenarch_1_18TGTACGTTGAGCGTACA plastid_5_18 GGCGCGAGCTCATC colwel_1_18CACGGATCAAGTCCA GATTTAAC CTTAGGCAGTG CGAACGGCTA crenarch_1_19CGTTGAGCGTACAGATT plastid_5_19 CGGCAGTCTCTCTA colwel_1_19CGCCACTCACGGATC TAACCGAA GAGATCCCAAT AAGTCCACGA crenarch_1_20GTACGTTGAGCGTACAG plastid_5_20 ATCACCGGCAGTCT colwel_1_20GCCACTCACGGATCA ATTTAACC CTCTAGAGATC AGTCCACGAA acido_1_15ACCTCTTCTGGAGTCCC margrpA_1_15 ACAACTGTATCCCG altero_3_15CTGTTGTCCCCCACGT CGAAGGGA AAGGATCCGCT TTTGGCATA acido_1_16CACCTCTTCTGGAGTCC margrpA_1_16 CAACTGTATCCCGA altero_3_16CTTGGGCTAATCAAA CCGAAGGG AGGATCCGCTG ACGCGCAAGG acido_1_17CGGCAGTCCCCCCAAAG margrpA_1_17 AACTGTATCCCGAA altero_3_17TCCCACTTGGGCTAAT TCCCCGGC GGATCCGCTGC CAAAACGCG acido_1_18CCCCGAAGGGGCCTTAC margrpA_1_18 AACAACTGTATCCC altero_3_18TTGGGCTAATCAAAA CGCTCAAC GAAGGATCCGC CGCGCAAGGC acido_1_19CCTCTTCTGGAGTCCCC margrpA_1_19 GTTAGCTCCGGTAC altero_3_19CCCACTTGGGCTAAT GAAGGGAA CGAAGGGGTCG CAAAACGCGC acido_1_20GGCAGTCCCCCCAAAGT margrpA_1_20 TTAGCTCCGGTACC altero_3_20TCACCGGCAGTCTCC CCCCGGCA GAAGGGGTCGA CTATAGTTCC acido_1_21AGCCATGCAGCACCTCT margrpA_1_21 GCGTTAGCTCCGGT altero_3_21TGGGCTAATCAAAAC TCTGGAGT ACCGAAGGGGT GCGCAAGGCC acido_1_22CAGCCATGCAGCACCTC margrpA_1_22 CGTTAGCTCCGGTA altero_3_22CCACTTGGGCTAATC TTCTGGAG CCGAAGGGGTC AAAACGCGCA acido_1_23CCCCCGAAGGGGCCTTA margrpA_1_23 TGCGTTAGCTCCGGT altero_3_23ATAGTTCCCGACATA CCGCTCAA ACCGAAGGGG ACTCGCTGGC acido_1_24ACAGCCATGCAGCACCT margrpA_1_24 TCCCTTACGACAGA altero_3_24CCATCGCTGGTTAGC CTTCTGGA CCTTTACGCTC AACCCTTTGT acido_1_25CCGAAGGGGCCTTACCG margrpA_1_25 ACTGTATCCCGAAG altero_3_25GGGCTAATCAAAACG CTCAACTT GATCCGCTGCA CGCAAGGCCC acido_2_1GTCAACTCCCTCCACAC margrpA_2_1 GCTGCCTTCGCATTT gamma_1_1 CTAAAAGGTCAAGCCCAAGTGTT GACTTTCCTC TCCCAACGGC acido_2_2 GGTCAACTCCCTCCACA margrpA_2_2GGCTGCCTTCGCATT gamma_1_2 ACTAAAAGGTCAAGC CCAAGTGT TGACTTTCCT CTCCCAACGGacido_2_3 GGGTCAACTCCCTCCAC margrpA_2_3 AGGCTGCCTTCGCA gamma_1_3GAAGAGGCCCTCTTT ACCAAGTG TTTGACTTTCC CCCTCTTAAG acido_2_4TCAACTCCCTCCACACC margrpA_2_4 ACAACTGTGCTCCG gamma_1_4 CACTAAAAGGTCAAGAAGTGTTC AAGAGCCCGCT CCTCCCAACG acido_2_5 GGGGTCAACTCCCTCCA margrpA_2_5TAACAACTGTGCTC gamma_1_5 GCATGTATTAGGCCT CACCAAGT CGAAGAGCCCG GCCGCCAACGacido_2_6 AGGGGTCAACTCCCTCC margrpA_2_6 AACAACTGTGCTCC gamma_1_6GGCTCCTCCAATAGT ACACCAAG GAAGAGCCCGC GAGAGCTTTC acido_2_7CAACTCCCTCCACACCA margrpA_2_7 GATACCATCTTCGG gamma_1_7 AAGAGGCCCTCTTTCAGTGTTCA GTACTGCAGAC CCTCTTAAGG acido_2_8 AAGGGGTCAACTCCCTC margrpA_2_8TTAACAACTGTGCTC gamma_1_8 CAAGAAGAGGCCCTC CACACCAA CGAAGAGCCC TTTCCCTCTTacido_2_9 GAAGGGGTCAACTCCCT margrpA_2_9 CAACTGTGCTCCGA gamma_1_9TCAAGAAGAGGCCCT CCACACCA AGAGCCCGCTG CTTTCCCTCT acido_2_10AACTCCCTCCACACCAA margrpA_2_10 CAGAAGGCTGCCTT gamma_1_10 TAGCTGCGCCACTAAGTGTTCAT CGCATTTGACT AAGGTCAAGC acido_2_11 ACTCCCTCCACACCAAGmargrpA_2_11 ACCATCTTCGGGTA gamma_1_11 CAGGCTCCTCCAATA TGTTCATCCTGCAGACTTC GTGAGAGCTT acido_2_12 CTCCCTCCACACCAAGT margrpA_2_12TTGCGGTTAGGATA gamma_1_12 CTCAGCGTCAGTATC GTTCATCG CCATCTTCGGGAATCCAGGGG acido_2_13 CAGTCCCCGTAGAGTTC margrpA_2_13 CTTGCGGTTAGGATgamma_1_13 AAAGGTCAAGCCTCC CCGCCATG ACCATCTTCGG CAACGGCTAG acido_2_14TCCCCGTAGAGTTCCCG margrpA_2_14 CCTTGCGGTTAGGAT gamma_1_14GCGTTAGCTGCGCCA CCATGACG ACCATCTTCG CTAAAAGGTC acido_2_15GTCCCCGTAGAGTTCCC margrpA_2_15 CCATCTTCGGGTACT gamma_1_15GAGGCCCTCTTTCCCT GCCATGAC GCAGACTTCC CTTAAGGCG acido_2_16AGTCCCCGTAGAGTTCC margrpA_2_16 GGATACCATCTTCG gamma_1_16AGAGGCCCTCTTTCCC CGCCATGA GGTACTGCAGA TCTTAAGGC acido_2_17GCAGTCCCCGTAGAGTT margrpA_2_17 ACCTGCCTTACCTTA gamma_1_17CCCCCTCTATCGTACT CCCGCCAT AACAGCTCCC CTAGCCTAT acido_2_18GGCAGTCCCCGTAGAGT margrpA_2_18 CCTGCCTTACCTTAA gamma_1_18CCCCTCTATCGTACTC TCCCGCCA ACAGCTCCCT TAGCCTATC acido_2_19CCGGCACGGAAGGGGT margrpA_2_19 CCAGAAGGCTGCCT gamma_1_19 TTCAAGAAGAGGCCCCAACTCCCT TCGCATTTGAC TCTTTCCCTC acido_2_20 ACGCGCTGGCAACTACGmargrpA_2_20 TGCGGTTAGGATAC gamma_1_20 AGGCCCTCTTTCCCTC GGTAAGGGCATCTTCGGGT TTAAGGCGT acido_2_21 GACGCGCTGGCAACTAC margrpA_2_21CGAAGAGCCCGCTG gamma_1_21 GCCCTCTTTCCCTCTT GGGTAAGG CATTATTTGGTAAGGCGTAT acido_2_22 TGACGCGCTGGCAACTA margrpA_2_22 CCACCATGAATTCTgamma_1_22 CCCTCTTTCCCTCTTA CGGGTAAG GCGTTCCTCTC AGGCGTATG acido_2_23AGCTCCGGCACGGAAG margrpA_2_23 CCTCCTTGCGGTTAG gamma_1_23CTCTTTCCCTCTTAAG GGGTCAACT GATACCATCT GCGTATGCG acido_2_24GCTCCGGCACGGAAGG margrpA_2_24 CATCTTCGGGTACTG gamma_1_24CCTCTTTCCCTCTTAA GGTCAACTC CAGACTTCCA GGCGTATGC acido_2_25CTCCGGCACGGAAGGG margrpA_2_25 CGGTTAGGATACCA gamma_1_25 GGCCCTCTTTCCCTCTGTCAACTCC TCTTCGGGTAC TAAGGCGTA acido_3_1 CTCACGGCATTCGTCCC OP10_1_1CCGCTTGCACGGGC gamma_2_1 TACCTGCTAGCAACC ACTCGACA AGTTCCGTAAG AGGGATAGGGacido_3_2 CGAGGTCCCCACGGTGT OP10_1_2 CCCGCTTGCACGGG gamma_2_2CAGCATTACCTGCTA CATGCGGT CAGTTCCGTAA GCAACCAGGG acido_3_3TCACCCTCACGGCATTC OP10_1_3 CGCTTGCACGGGCA gamma_2_3 TTACCTGCTAGCAACGTCCCACT GTTCCGTAAGA CAGGGATAGG acido_3_4 AGGTCCCCACGGTGTCA OP10_1_4TCCCGCTTGCACGG gamma_2_4 ACCTGCTAGCAACCA TGCGGTAT GCAGTTCCGTA GGGATAGGGGacido_3_5 GGACCGAGGTCCCCAC OP10_1_5 GGGTGCAGACAATT gamma_2_5TCAGCATTACCTGCTA GGTGTCATG CAGGTGACTTG GCAACCAGG acido_3_6CCGAGGTCCCCACGGTG OP10_1_6 CTCCCGCTTGCACG gamma_2_6 TCTCCCTGGAGTTCTCTCATGCGG GGCAGTTCCGT AGCATTACC acido_3_7 ACCCTCACGGCATTCGT OP10_1_7CCTCCCGCTTGCACG gamma_2_7 GTCTCCCTGGAGTTCT CCCACTCG GGCAGTTCCG CAGCATTACacido_3_8 ACCGAGGTCCCCACGGT OP10_1_8 GCTTGCACGGGCAG gamma_2_8CAGTCTCCCTGGAGTT GTCATGCG TTCCGTAAGAG CTCAGCATT acido_3_9CACCCTCACGGCATTCG OP10_1_9 CGGGTGCAGACAAT gamma_2_9 TCCCTGGAGTTCTCAGTCCCACTC TCAGGTGACTT CATTACCTG acido_3_10 GACCGAGGTCCCCACG OP10_1_10CCGTAAGAGTTCCC gamma_2_10 CTCCCTGGAGTTCTCA GTGTCATGC GACTTTACGCTGCATTACCT acido_3_11 CCTCACGGCATTCGTCC OP10_1_11 GCAGACAATTCAGGgamma_2_11 GCAGTCTCCCTGGAG CACTCGAC TGACTTGACGG TTCTCAGCAT acido_3_12TTCACCCTCACGGCATT OP10_1_12 TCGGGTGCAGACAA gamma_2_12 GGCAGTCTCCCTGGACGTCCCAC TTCAGGTGACT GTTCTCAGCA acido_3_13 GAGGTCCCCACGGTGTC OP10_1_13CGTAAGAGTTCCCG gamma_2_13 CCTGCTAGCAACCAG ATGCGGTA ACTTTACGCTGGGATAGGGGT acido_3_14 CCCTCACGGCATTCGTC OP10_1_14 TTGCACGGGCAGTTgamma_2_14 TGCTAGCAACCAGGG CCACTCGA CCGTAAGAGTT ATAGGGGTTG acido_3_15GGTCCCCACGGTGTCAT OP10_1_15 TCCGTAAGAGTTCC gamma_2_15 CTGCTAGCAACCAGGGCGGTATT CGACTTTACGC GATAGGGGTT acido_3_16 GTCCCCACGGTGTCATG OP10_1_16GGCAGTTCCGTAAG gamma_2_16 TAGCAACCAGGGATA CGGTATTA AGTTCCCGACTGGGGTTGCGC acido_3_17 GATTGTTCACCCTCACG OP10_1_17 CTTGCACGGGCAGTgamma_2_17 AGCAACCAGGGATAG GCATTCGT TCCGTAAGAGT GGGTTGCGCT acido_3_18AGGACCGAGGTCCCCA OP10_1_18 CGGGCAGTTCCGTA gamma_2_18 CTCAGCATTACCTGCTCGGTGTCAT AGAGTTCCCGA AGCAACCAG acido_3_19 ATTGTTCACCCTCACGG OP10_1_19TGCACGGGCAGTTC gamma_2_19 CTAGCAACCAGGGAT CATTCGTC CGTAAGAGTTCAGGGGTTGCG acido_3_20 TTGTTCACCCTCACGGC OP10_1_20 ACGGGCAGTTCCGTgamma_2_20 GCTAGCAACCAGGGA ATTCGTCC AAGAGTTCCCG TAGGGGTTGC acido_3_21TGTTCACCCTCACGGCA OP10_1_21 GCACGGGCAGTTCC gamma_2_21 GCATTACCTGCTAGCTTCGTCCC GTAAGAGTTCC AACCAGGGAT acido_3_22 GGATTGTTCACCCTCAC OP10_1_22CACGGGCAGTTCCG gamma_2_22 AGCATTACCTGCTAG GGCATTCG TAAGAGTTCCCCAACCAGGGA acido_3_23 CACGGCATTCGTCCCAC OP10_1_23 GCAGTTCCGTAAGAgamma_2_23 TCGCGAGTTGGCAGC TCGACAGG GTTCCCGACTT CCTCTGTACG acido_3_24TCACGGCATTCGTCCCA OP10_1_24 GGGCAGTTCCGTAA gamma_2_24 CTCGCGAGTTGGCAGCTCGACAG GAGTTCCCGAC CCCTCTGTAC acido_3_25 GCTTTGATCGCAAGGAC OP10_1_25CCCCCTTACTCCCCA gamma_2_25 CGCGAGTTGGCAGCC CGAGGTCC CACCTTAGACCTCTGTACGC actino_1_1 AAACCTAGATCCGTCAT OP3_1_1 ATCCAAGGGTGATA gamma_3_1TGCGACACCGAAGGG CCCACACG GGTCCTTACGG CAACCCCCCC actino_1_2CAAACCTAGATCCGTCA OP3_1_2 TCCAAGGGTGATAG gamma_3_2 CTGCGACACCGAAGGTCCCACAC GTCCTTACGGA GCAACCCCCC actino_1_3 CACCACCTGTATAGGGC OP3_1_3CCAAGGGTGATAGG gamma_3_3 GACTAGTTCCGAGTA GCTAATGC TCCTTACGGAT TGTCAAGGGCactino_1_4 ACCACCTGTATAGGGCG OP3_1_4 TGTTCTCCCCTGCTG gamma_3_4GCTGCGACACCGAAG CTAATGCA ACAGGAGTTT GGCAACCCCC actino_1_5CCACCTGTATAGGGCGC OP3_1_5 TTGTTCTCCCCTGCT gamma_3_5 AACGCGCTAGCTGCGTAATGCAC GACAGGAGTT ACACCGAAGG actino_1_6 CACCTGTATAGGGCGCT OP3_1_6CTTGTTCTCCCCTGC gamma_3_6 TAACGCGCTAGCTGC AATGCACA TGACAGGAGT GACACCGAAGactino_1_7 GCACCACCTGTATAGGG OP3_1_7 GTTCTCCCCTGCTGA gamma_3_7TTACTTAACCGCCAA CGCTAATG CAGGAGTTTA CGCGCGCTTT actino_1_8AACCTAGATCCGTCATC OP3_1_8 CATCCAAGGGTGAT gamma_3_8 ACGCGCTAGCTGCGACCACACGC AGGTCCTTACG CACCGAAGGG actino_1_9 TGCACCACCTGTATAGG OP3_1_9TCGACAGGTTATCC gamma_3_9 TTAACGCGCTAGCTG GCGCTAAT CGAACCCTAGG CGACACCGAAactino_1_10 AGCCCTGAACTTTCACG OP3_1_10 TTCGACAGGTTATCC gamma_3_10CGCGCTAGCTGCGAC ACCGACTT CGAACCCTAG ACCGAAGGGC actino_1_11GCCCTGAACTTTCACGA OP3_1_11 TTCTCCCCTGCTGAC gamma_3_11 TACTTAACCGCCAACCCGACTTG AGGAGTTTAC GCGCGCTTTA actino_1_12 GAGCCCTGAACTTTCAC OP3_1_12CCATCCAAGGGTGA gamma_3_12 AGCTGCGACACCGAA GACCGACT TAGGTCCTTACGGGCAACCCC actino_1_13 AGCGTCGATAGCGGCCC OP3_1_13 TGATAGGTCCTTACGgamma_3_13 CTTACTTAACCGCCA AGTGAGCT GATCCCCATC ACGCGCGCTT actino_1_14GCGTCGATAGCGGCCCA OP3_1_14 TCTCCCCTGCTGACA gamma_3_14 ATCCGACTTACTTAACGTGAGCTG GGAGTTTACA CGCCAACGC actino_1_15 CGTCGATAGCGGCCCAG OP3_1_15CGGATCCCCATCTTT gamma_3_15 CGACTTACTTAACCG TGAGCTGC CCCTCATGTTCCAACGCGCG actino_1_16 CAGCGTCGATAGCGGCC OP3_1_16 TCCTTGCCGGTTAGGgamma_3_16 TCCGACTTACTTAACC CAGTGAGC CAACCTACTT GCCAACGCG actino_1_17CCCTGAACTTTCACGAC OP3_1_17 AGTGCGCACCGACC gamma_3_17 CTTAACGCGCTAGCTCGACTTGT GAAGTCGGTGT GCGACACCGA actino_1_18 TGAGCCCTGAACTTTCA OP3_1_18CCAGTAATGCGCCT gamma_3_18 ACTTACTTAACCGCC CGACCGAC TCGCGACTGGTAACGCGCGCT actino_1_19 ACCTAGATCCGTCATCC OP3_1_19 AGAGTGCGCACCGAgamma_3_19 GCGCTAGCTGCGACA CACACGCG CCGAAGTCGGT CCGAAGGGCA actino_1_20CTCGGGCTATCCCAGTA OP3_1_20 TCGAAAAGCACAGG gamma_3_20 CCGACTTACTTAACCACTAAGGT ACGTATCCGGT GCCAACGCGC actino_1_21 CCTCGGGCTATCCCAGT OP3_1_21CTGTGCTTCGAAAA gamma_3_21 ACTTAACCGCCAACG AACTAAGG GCACAGGACGTCGCGCTTTAC actino_1_22 TCGATAGCGGCCCAGTG OP3_1_22 CCTTAGAGTGCGCAgamma_3_22 CATCCGACTTACTTAA AGCTGCCT CCGACCGAAGT CCGCCAACG actino_1_23GTCGATAGCGGCCCAGT OP3_1_23 GCCCTCCTTGCCGGT gamma_3_23 TCTTCACACACGCGGGAGCTGCC TAGGCAACCT CATTGCTAGA actino_1_24 CGATAGCGGCCCAGTG OP3_1_24CTCCTTGCCGGTTAG gamma_3_24 AGAACTTAACGCGCT AGCTGCCTT GCAACCTACTAGCTGCGACA actino_1_25 TCCTCGGGCTATCCCAG OP3_1_25 CAGTAATGCGCCTTgamma_3_25 ACTTAACGCGCTAGC TAACTAAG CGCGACTGGTG TGCGACACCG actino_2_1CCGGTTTCCCCAAGTGC OP9_1_1 GGGCAAGATAATGT gamma_4_1 ACACCGAAAGGCAAAAAGCACTT CAAGTCCCGGT CCCTCCCGAC actino_2_2 CAAGCACTTGGTTCGTC OP9_1_2GCTGGCACATAATT gamma_4_2 GACACCGAAAGGCAA CCTCGACT AGCCGGAGCTT ACCCTCCCGAactino_2_3 GCCGGTTTCCCCAAGTG OP9_1_3 TGCTGGCACATAATT gamma_4_3CACCGAAAGGCAAAC CAAGCACT AGCCGGAGCT CCTCCCGACA actino_2_4GCTTCGACACGGAAATC OP9_1_4 CCCACTTACAGGGT gamma_4_4 ACCGAAAGGCAAACCGTGAACTG AGATTACCCAC CTCCCGACAT actino_2_5 TTCGCCGGTTTCCCCAA OP9_1_5CCCCACTTACAGGG gamma_4_5 CGACACCGAAAGGCA GTGCAAGC TAGATTACCCA AACCCTCCCGactino_2_6 CGACACGGAAATCGTG OP9_1_6 CCCCCACTTACAGG gamma_4_6CCGAAAGGCAAACCC AACTGATCC GTAGATTACCC TCCCGACATC actino_2_7GACACGGAAATCGTGA OP9_1_7 CTGCTAACCTCATCA gamma_4_7 GCGACACCGAAAGGCACTGATCCC TCCCGAAGGA AAACCCTCCC actino_2_8 ACACGGAAATCGTGAA OP9_1_8TCTGCTAACCTCATC gamma_4_8 CGAAAGGCAAACCCT CTGATCCCC ATCCCGAAGGCCCGACATCT actino_2_9 CGCCGGTTTCCCCAAGT OP9_1_9 CTGCTGGCACATAA gamma_4_9GCTGCGACACCGAAA GCAAGCAC TTAGCCGGAGC GGCAAACCCT actino_2_10ACGGAAATCGTGAACT OP9_1_10 CCACTTACAGGGTA gamma_4_10 AGCTGCGACACCGAAGATCCCCAC GATTACCCACG AGGCAAACCC actino_2_11 TCGCCGGTTTCCCCAAG OP9_1_11GACGGGCAAGATAA gamma_4_11 TTGGCTAGCCATTGCT TGCAAGCA TGTCAAGTCCCGGTTTGCAG actino_2_12 CACGGAAATCGTGAACT OP9_1_12 TCCCCCACTTACAGgamma_4_12 TGGCTAGCCATTGCT GATCCCCA GGTAGATTACC GGTTTGCAGC actino_2_13CGGTTTCCCCAAGTGCA OP9_1_13 GCAGTCTGCCTAGA gamma_4_13 GGATTGGCTAGCCATAGCACTTG GTGCACTTGTA TGCTGGTTTG actino_2_14 AAGTGCAAGCACTTGGT OP9_1_14GCTGCTGGCACATA gamma_4_14 GATTGGCTAGCCATT TCGTCCCT ATTAGCCGGAGGCTGGTTTGC actino_2_15 GTTCGCCGGTTTCCCCA OP9_1_15 GGGTACCGTCAGGCgamma_4_15 GGGATTGGCTAGCCA AGTGCAAG TTAAGGGTTTA TTGCTGGTTT actino_2_16CGGAAATCGTGAACTG OP9_1_16 CACTTACAGGGTAG gamma_4_16 GGCTAGCCATTGCTGATCCCCACA ATTACCCACGC GTTTGCAGCC actino_2_17 GCAAGCACTTGGTTCGT OP9_1_17GGCAGTCTGCCTAG gamma_4_17 GAAAGGCAAACCCTC CCCTCGAC AGTGCACTTGTCCGACATCTA actino_2_18 CGTTCGCCGGTTTCCCC OP9_1_18 GGTTATCCCCCACTTgamma_4_18 CTGCGACACCGAAAG AAGTGCAA ACAGGGTAGA GCAAACCCTC actino_2_19AAGCACTTGGTTCGTCC OP9_1_19 GAGGGTTATCCCCC gamma_4_19 TGCGACACCGAAAGGCTCGACTT ACTTACAGGGT CAAACCCTCC actino_2_20 GGTTTCCCCAAGTGCAA OP9_1_20GGGTTATCCCCCACT gamma_4_20 AGGGATTGGCTAGCC GCACTTGG TACAGGGTAGATTGCTGGTT actino_2_21 AGTGCAAGCACTTGGTT OP9_1_21 GTCAGAGATAGACCgamma_4_21 AAGGGATTGGCTAGC CGTCCCTC AGAAAGCCGCC CATTGCTGGT actino_2_22CAAGTGCAAGCACTTGG OP9_1_22 GGGGTACCGTCAGG gamma_4_22 TAAGGGATTGGCTAGTTCGTCCC CTTAAGGGTTT CCATTGCTGG actino_2_23 CCGTTCGCCGGTTTCCC OP9_1_23AGGGTTATCCCCCA gamma_4_23 TAGCTGCGACACCGA CAAGTGCA CTTACAGGGTAAAGGCAAACC actino_2_24 CCGTAGTTATCCCGGTG OP9_1_24 CGGCAGTCTGCCTAgamma_4_24 TTAGCTGCGACACCG TACAGGGC GAGTGCACTTG AAAGGCAAAC actino_2_25CCTCAAGCCTTGCAGTA OP9_1_25 CTCCGCATTATCTGC gamma_4_25 GTTAGCTGCGACACCTCGACTGC GGCAGTCTGC GAAAGGCAAA bacter_1_1 GTTTCCGCGACTGTCAT plancto_1_1TGCAACACCTGTGC gamma_5_1 CCACTAAGGGACAAA TCCACGTT AGGTCACACCC TTCCCCCAACbacter_1_2 TTCCGCGACTGTCATTC plancto_1_2 GCAACACCTGTGCA gamma_5_2CGCCACTAAGGGACA CACGTTCG GGTCACACCCG AATTCCCCCA bacter_1_3ACGTTTCCGCGACTGTC plancto_1_3 ATGCAACACCTGTG gamma_5_3 GCCACTAAGGGACAAATTCCACG CAGGTCACACC ATTCCCCCAA bacter_1_4 TTTCCGCGACTGTCATT plancto_1_4AACACCTGTGCAGG gamma_5_4 CACTAAGGGACAAAT CCACGTTC TCACACCCGAA TCCCCCAACGbacter_1_5 CACGTTTCCGCGACTGT plancto_1_5 CAACACCTGTGCAG gamma_5_5ACTAAGGGACAAATT CATTCCAC GTCACACCCGA CCCCCAACGG bacter_1_6TCACGTTTCCGCGACTG plancto_1_6 TGTGCAGGTCACAC gamma_5_6 CTAAGGGACAAATTCTCATTCCA CCGAAGGTAAT CCCCAACGGC bacter_1_7 CGTTTCCGCGACTGTCA plancto_1_7GTGCAGGTCACACC gamma_5_7 GCGCCACTAAGGGAC TTCCACGT CGAAGGTAATC AAATTCCCCCbacter_1_8 TGTCATTCCACGTTCGA plancto_1_8 TGCAGGTCACACCC gamma_5_8GGTACCGTCAAGACG GCCCAGGT GAAGGTAATCA CGCAGTTATT bacter_1_9CTGTCATTCCACGTTCG plancto_1_9 CTGTGCAGGTCACA gamma_5_9 AGGTACCGTCAAGACAGCCCAGG CCCGAAGGTAA GCGCAGTTAT bacter_1_10 CCGCGACTGTCATTCCAplancto_1_10 CCTGTGCAGGTCAC gamma_5_10 TAGGTACCGTCAAGA CGTTCGAGACCCGAAGGTA CGCGCAGTTA bacter_1_11 ACTGTCATTCCACGTTC plancto_1_11ACACCTGTGCAGGT gamma_5_11 TGCGCCACTAAGGGA GAGCCCAG CACACCCGAAGCAAATTCCCC bacter_1_12 CGCGACTGTCATTCCAC plancto_1_12 ACAGAGTTAGCCAGgamma_5_12 TAAGGGACAAATTCC GTTCGAGC TGCTTCCTCTC CCCAACGGCT bacter_1_13GCGACTGTCATTCCACG plancto_1_13 ACCTGTGCAGGTCA gamma_5_13 CTGTAGGTACCGTCATTCGAGCC CACCCGAAGGT AGACGCGCAG bacter_1_14 CGACTGTCATTCCACGTplancto_1_14 CATGCAACACCTGT gamma_5_14 GTAGGTACCGTCAAG TCGAGCCCGCAGGTCACAC ACGCGCAGTT bacter_1_15 TCCGCGACTGTCATTCC plancto_1_15CACCTGTGCAGGTC gamma_5_15 CTGCGCCACTAAGGG ACGTTCGA ACACCCGAAGGACAAATTCCC bacter_1_16 GACTGTCATTCCACGTT plancto_1_16 CACAGAGTTAGCCAgamma_5_16 TGTAGGTACCGTCAA CGAGCCCA GTGCTTCCTCT GACGCGCAGT bacter_1_17ATCACGTTTCCGCGACT plancto_1_17 CAGAGTTAGCCAGT gamma_5_17 TCTGTAGGTACCGTCGTCATTCC GCTTCCTCTCG AAGACGCGCA bacter_1_18 GTCATTCCACGTTCGAGplancto_1_18 AGCCAGTGCTTCCTC gamma_5_18 GTCCGCCACTCGACG CCCAGGTATCGAGCTTAC CCTGAAGAGC bacter_1_19 ACGGTACCATCAGCACC plancto_1_19GCACAGAGTTAGCC gamma_5_19 GCCACTCGACGCCTG GATACACG AGTGCTTCCTCAAGAGCAAGC bacter_1_20 GTACCATCAGCACCGAT plancto_1_20 GGCCTAGCCCCTGCgamma_5_20 GCTGCGCCACTAAGG ACACGACC ATGTCAAGCCT GACAAATTCC bacter_1_21GGTACCATCAGCACCGA plancto_1_21 GCAGGTCACACCCG gamma_5_21 CACTCGGTTCCCGAATACACGAC AAGGTAATCAG GGCACCAAAC bacter_1_22 CGGTACCATCAGCACCGplancto_1_22 ACCGGCCTAGCCCC gamma_5_22 CTTCTGTAGGTACCGT ATACACGATGCATGTCAAG CAAGACGCG bacter_1_23 GATCACGTTTCCGCGAC plancto_1_23CAGGTCACACCCGA gamma_5_23 CACTCGACGCCTGAA TGTCATTC AGGTAATCAGCGAGCAAGCTC bacter_1_24 TACGGTACCATCAGCAC plancto_1_24 CCGGCCTAGCCCCTgamma_5_24 CGCCACTCGACGCCT CGATACAC GCATGTCAAGC GAAGAGCAAG bacter_1_25CACCGATACACGACCG plancto_1_25 CGGCCTAGCCCCTG gamma_5_25 GGACAAATTCCCCCAGTGGTTTTT CATGTCAAGCC ACGGCTAGTT bacter_2_1 GGATTTCTCCGGGCTACplancto_2_1 TCTCCGAAGAGCAC gamma_6_1 AGCTGCGCCACCAAC CTTCCGGTTCTCCCCTTTC CTCTTGAATG bacter_2_2 CTCCGGGCTACCTTCCG plancto_2_2TACGACCGAGAAAC gamma_6_2 CCAACCTCTTGAATG GTAAAGGG TGTGGGAGGTC AGGCCGACGGbacter_2_3 CGGATTTCTCCGGGCTA plancto_2_3 ACCGAGAAACTGTG gamma_6_3TGCGCCACCAACCTC CCTTCCGG GGAGGTCCCTC TTGAATGAGG bacter_2_4TCTCCGGGCTACCTTCC plancto_2_4 CGACCGAGAAACTG gamma_6_4 GCCACCAACCTCTTGGGTAAAGG TGGGAGGTCCC AATGAGGCCG bacter_2_5 TTCTCCGGGCTACCTTC plancto_2_5CTCCGAAGAGCACT gamma_6_5 ACCAACCTCTTGAAT CGGTAAAG CTCCCCTTTCA GAGGCCGACGbacter_2_6 TTTCTCCGGGCTACCTT plancto_2_6 GCCCGACCTTCCTCT gamma_6_6CTGCGCCACCAACCT CCGGTAAA GAGGTTTGGT CTTGAATGAG bacter_2_7GATTTCTCCGGGCTACC plancto_2_7 AAACTGTGGGAGGT gamma_6_7 CAACCTCTTGAATGATTCCGGTA CCCTCGATCCA GGCCGACGGC bacter_2_8 ATTTCTCCGGGCTACCT plancto_2_8TCCGAAGAGCACTC gamma_6_8 GCGCCACCAACCTCT TCCGGTAA TCCCCTTTCAG TGAATGAGGCbacter_2_9 CCGGATTTCTCCGGGCT plancto_2_9 GACCGAGAAACTGT gamma_6_9CGCCACCAACCTCTT ACCTTCCG GGGAGGTCCCT GAATGAGGCC bacter_2_10TCCGGATTTCTCCGGGC plancto_2_10 ACGACCGAGAAACT gamma_6_10 CACCAACCTCTTGAATACCTTCC GTGGGAGGTCC TGAGGCCGAC bacter_2_11 TCCGGGCTACCTTCCGGplancto_2_11 GAAACTGTGGGAGG gamma_6_11 GCTGCGCCACCAACC TAAAGGGTTCCCTCGATCC TCTTGAATGA bacter_2_12 ATCCGGATTTCTCCGGG plancto_2_12CTCTCCGAAGAGCA gamma_6_12 CCACCAACCTCTTGA CTACCTTC CTCTCCCCTTTATGAGGCCGA bacter_2_13 CTTTATGGATTAGCTCC plancto_2_13 GCCTGGAGGTAGGTgamma_6_13 TAGCTGCGCCACCAA CCGTCGCT ATCTACCTGTT CCTCTTGAAT bacter_2_14ACTTTATGGATTAGCTC plancto_2_14 TCCCGACGCTATTCC gamma_6_14AACCTCTTGAATGAG CCCGTCGC CAGCCTGGAG GCCGACGGCT bacter_2_15CCGGGCTACCTTCCGGT plancto_2_15 TTGGGCATTACCGC gamma_6_15 AGAGGTCCACTTTGCAAAGGGTA CAGTTTCCCGA CCCGAAGGGC bacter_2_16 AATCCGGATTTCTCCGGplancto_2_16 CCGAGAAACTGTGG gamma_6_16 GAGGTCCACTTTGCC GCTACCTTGAGGTCCCTCG CCGAAGGGCG bacter_2_17 GCTACCTTCCGGTAAAG plancto_2_17TGAGCAGACCCATC gamma_6_17 TCTTCAGGTAACGTC GGTAGGTT TCCAGGCGCCGAATACGCGCG bacter_2_18 GGCTACCTTCCGGTAAA plancto_2_18 AACTGTGGGAGGTCgamma_6_18 TTAGCTGCGCCACCA GGGTAGGT CCTCGATCCAG ACCTCTTGAA bacter_2_19GGGCTACCTTCCGGTAA plancto_2_19 CCCGACCTTCCTCTG gamma_6_19CAGAGGTCCACTTTG AGGGTAGG AGGTTTGGTC CCCCGAAGGG bacter_2_20TAATCCGGATTTCTCCG plancto_2_20 TGGGCATTACCGCC gamma_6_20AGGTCCACTTTGCCCC GGCTACCT AGTTTCCCGAC GAAGGGCGT bacter_2_21CTACCTTCCGGTAAAGG plancto_2_21 CGAGAAACTGTGGG gamma_6_21 ACCTCTTGAATGAGGGTAGGTTG AGGTCCCTCGA CCGACGGCTA bacter_2_22 CGGGCTACCTTCCGGTAplancto_2_22 GAGAAACTGTGGGA gamma_6_22 CGCGCGGGTATTAAC AAGGGTAGGGTCCCTCGAT CGCACGCTTT bacter_2_23 TTAATCCGGATTTCTCC plancto_2_23CAGCCTGGAGGTAG gamma_6_23 CTTCAGGTAACGTCA GGGCTACC GTATCTACCTGATACGCGCGG bacter_2_24 TTTATGGATTAGCTCCC plancto_2_24 AGCCCGACCTTCCTCgamma_6_24 TCAGAGGTCCACTTT CGTCGCTG TGAGGTTTGG GCCCCGAAGG bacter_2_25TACCTTCCGGTAAAGGG plancto_2_25 AATAGTGAGCAGAC gamma_6_25 ACGCGCGGGTATTAATAGGTTGC CCATCTCCAGG CCGCACGCTT bacter_3_1 GGCTCCTCGCCGTATCA plancto_3_1CGCAGTGCCTCAGT gamma_7_1 GTCCTCCGTAGTTAG TCGAAATT TAAGCTCAGGC ACTAGCCACTbacter_3_2 CAACCTTGCCAATCACT plancto_3_2 GCAGTGCCTCAGTT gamma_7_2CGTCCTCCGTAGTTAG CCCCAGGT AAGCTCAGGCA ACTAGCCAC bacter_3_3CTTGCCAATCACTCCCC plancto_3_3 CAACTCTGAGGGAG gamma_7_3 ACCGTCCTCCGTAGTTAGGTGGAT TACCCTCAGAG AGACTAGCC bacter_3_4 CAGGTAAGGCTCCTCGC plancto_3_4GTCAACTCTGAGGG gamma_7_4 CCGTCCTCCGTAGTTA CGTATCAT AGTACCCTCAG GACTAGCCAbacter_3_5 AGGCTCCTCGCCGTATC plancto_3_5 TATGTTTTCCTACGC gamma_7_5GACCGTCCTCCGTAG ATCGAAAT CGTTCGCCGC TTAGACTAGC bacter_3_6AACCTTGCCAATCACTC plancto_3_6 GCAGAAAGAGGAAA gamma_7_6 TGACCGTCCTCCGTACCCAGGTG CCTCCTCCCGC GTTAGACTAG bacter_3_7 ACCTTGCCAATCACTCC plancto_3_7AACTCTGAGGGAGT gamma_7_7 CTGCAGGTAACGTCA CCAGGTGG ACCCTCAGAGA AGTACTCACCbacter_3_8 TCAACCTTGCCAATCAC plancto_3_8 TCAACTCTGAGGGA gamma_7_8TATTAGGGGTAAGCC TCCCCAGG GTACCCTCAGA TTCCTCCCTG bacter_3_9GGTAAGGCTCCTCGCCG plancto_3_9 CTATGTTTTCCTACG gamma_7_9 TGCAGGTAACGTCAATATCATCG CCGTTCGCCG GTACTCACCC bacter_3_10 TCCGCCTACCCCAACTAplancto_3_10 TCCTATGTTTTCCTA gamma_7_10 GCAGGTAACGTCAAG TACTCTAGCGCCGTTCGC TACTCACCCG bacter_3_11 TTCAACCTTGCCAATCA plancto_3_11CCTATGTTTTCCTAC gamma_7_11 TTCCCCGGGTTGTCCC CTCCCCAG GCCGTTCGCCCCACTCATG bacter_3_12 CCCAGGTAAGGCTCCTC plancto_3_12 ACTCTGAGGGAGTAgamma_7_12 TCCCCGGGTTGTCCCC GCCGTATC CCCTCAGAGAT CACTCATGG bacter_3_13AGGTAAGGCTCCTCGCC plancto_3_13 ACGCAGTGCCTCAG gamma_7_13CCCCGGGTTGTCCCCC GTATCATC TTAAGCTCAGG ACTCATGGG bacter_3_14CCAATCACTCCCCAGGT plancto_3_14 TGTCAACTCTGAGG gamma_7_14TTTCCCCGGGTTGTCC GGATTACC GAGTACCCTCA CCCACTCAT bacter_3_15CCTTGCCAATCACTCCC plancto_3_15 ATGTTTTCCTACGCC gamma_7_15CCCGGGTTGTCCCCC CAGGTGGA GTTCGCCGCT ACTCATGGGT bacter_3_16GTAAGGCTCCTCGCCGT plancto_3_16 AACGCAGTGCCTCA gamma_7_16 CCGGGTTGTCCCCCAATCATCGA GTTAAGCTCAG CTCATGGGTA bacter_3_17 CCGCCTACCCCAACTATplancto_3_17 CAGTGCCTCAGTTA gamma_7_17 CTCACCCGTATTAGG ACTCTAGAAGCTCAGGCAT GGTAAGCCTT bacter_3_18 CCAGGTAAGGCTCCTCG plancto_3_18CTGTCAACTCTGAG gamma_7_18 ACCCGTATTAGGGGT CCGTATCA GGAGTACCCTCAAGCCTTCCT bacter_3_19 AAGGCTCCTCGCCGTAT plancto_3_19 CTCTGAGGGAGTACgamma_7_19 ACTCACCCGTATTAG CATCGAAA CCTCAGAGATT GGGTAAGCCT bacter_3_20GCCAATCACTCCCCAGG plancto_3_20 TCTGTCAACTCTGAG gamma_7_20GTCAAGTACTCACCC TGGATTAC GGAGTACCCT GTATTAGGGG bacter_3_21TAAGGCTCCTCGCCGTA plancto_3_21 GGAGTACCCTCAGA gamma_7_21 TCACCCGTATTAGGGTCATCGAA GATTTCATCCC GTAAGCCTTC bacter_3_22 GCCCAGGTAAGGCTCCTplancto_3_22 CAAACGCAGTGCCT gamma_7_22 CCCGTATTAGGGGTA CGCCGTATCAGTTAAGCTC AGCCTTCCTC bacter_3_23 CATTCCGCCTACCCCAA plancto_3_23CTCTGTCAACTCTGA gamma_7_23 GTACTCACCCGTATTA CTATACTC GGGAGTACCCGGGGTAAGC bacter_3_24 CAATCACTCCCCAGGTG plancto_3_24 ACAGCAGAAAGAGGgamma_7_24 CACCCGTATTAGGGG GATTACCT AAACCTCCTCC TAAGCCTTCC bacter_3_25CCGCCGGAACTTTGATC plancto_3_25 CTGAGGGAGTACCC gamma_7_25TACTCACCCGTATTAG ATCAAGAG TCAGAGATTTC GGGTAAGCC flavo_1_1CTCAGACACCAAGGTCC plancto_4_1 ACTACCTAATATCG gamma_8_1 CGCGAGCTCATCCATAAACAGCT CATCGGCCGCT CAGCACAAGG flavo_1_2 CAGACACCAAGGTCCA plancto_4_2CAACTACCTAATAT gamma_8_2 TCATCCATCAGCACA AACAGCTAG CGCATCGGCCGAGGTCCGAAG flavo_1_3 CACTCAGACACCAAGGT plancto_4_3 AACTACCTAATATCgamma_8_3 CTCATCCATCAGCAC CCAAACAG GCATCGGCCGC AAGGTCCGAA flavo_1_4GCTTAGCCACTCAGACA plancto_4_4 CCAACTACCTAATA gamma_8_4 GCTCATCCATCAGCACCAAGGTC TCGCATCGGCC CAAGGTCCGA flavo_1_5 ACTCAGACACCAAGGTC plancto_4_5ACGTTCCGATGTATT gamma_8_5 ACGCGAGCTCATCCA CAAACAGC CCTACCCCGT TCAGCACAAGflavo_1_6 CTTAGCCACTCAGACAC plancto_4_6 TACGTTCCGATGTAT gamma_8_6CATCCATCAGCACAA CAAGGTCC TCCTACCCCG GGTCCGAAGA flavo_1_7TACCGTCAAGCTTGGTA plancto_4_7 GTACGTTCCGATGTA gamma_8_7 GACGCGAGCTCATCCCACGTACC TTCCTACCCC ATCAGCACAA flavo_1_8 GTACCGTCAAGCTTGGT plancto_4_8CTACCTAATATCGC gamma_8_8 GCGAGCTCATCCATC ACACGTAC ATCGGCCGCTC AGCACAAGGTflavo_1_9 GCCACTCAGACACCAA plancto_4_9 CGTTCCGATGTATTC gamma_8_9TCCATCAGCACAAGG GGTCCAAAC CTACCCCGTT TCCGAAGATC flavo_1_10TTAGCCACTCAGACACC plancto_4_10 GTTTCCACCCACTAA gamma_8_10CGACGCGAGCTCATC AAGGTCCA TCCGTGCATG CATCAGCACA flavo_1_11ACCGTCAAGCTTGGTAC plancto_4_11 TTCCACCCACTAATC gamma_8_11CATCAGCACAAGGTC ACGTACCA CGTGCATGTC CGAAGATCCC flavo_1_12CCACTCAGACACCAAG plancto_4_12 TCCACCCACTAATCC gamma_8_12 CCCTCTAATGGGCAGGTCCAAACA GTGCATGTCA ATTCTCACGT flavo_1_13 AGCCACTCAGACACCA plancto_4_13CCACCCACTAATCC gamma_8_13 CCGACGCGAGCTCAT AGGTCCAAA GTGCATGTCAACCATCAGCAC flavo_1_14 TAGCCACTCAGACACCA plancto_4_14 GGCAGTAAACCTTTgamma_8_14 CCCCTCTAATGGGCA AGGTCCAA GGTCTCTCGAC GATTCTCACG flavo_1_15CCGTCAAGCTTGGTACA plancto_4_15 GGTACGTTCCGATGT gamma_8_15CCCCCTCTAATGGGC CGTACCAA ATTCCTACCC AGATTCTCAC flavo_1_16CGCTTAGCCACTCAGAC plancto_4_16 TGCGAGCGTCATGA gamma_8_16 CGAGCTCATCCATCAACCAAGGT ATGTTTCCACC GCACAAGGTC flavo_1_17 TCGCTTAGCCACTCAGAplancto_4_17 GCGAGCGTCATGAA gamma_8_17 CCATCAGCACAAGGT CACCAAGGTGTTTCCACCC CCGAAGATCC flavo_1_18 CGTCAAGCTTGGTACAC plancto_4_18GAGCGTCATGAATG gamma_8_18 CCTCTAATGGGCAGA GTACCAAG TTTCCACCCACTTCTCACGTG flavo_1_19 CAGCTAGTAACCATCGT plancto_4_19 CGAGCGTCATGAATgamma_8_19 CCCAGGTTATCCCCCT TTACCGGC GTTTCCACCCA CTAATGGGC flavo_1_20GCCATAGCTAGAGACTA plancto_4_20 CAGTTATGCCCCAG gamma_8_20 TCCGACGCGAGCTCATGGGGGAT TGAATCGCCTT TCCATCAGCA flavo_1_21 TGCCATAGCTAGAGACTplancto_4_21 TCAGTTATGCCCCA gamma_8_21 GAGCTCATCCATCAG ATGGGGGAGTGAATCGCCT CACAAGGTCC flavo_1_22 ATGCCATAGCTAGAGAC plancto_4_22AGTTATGCCCCAGT gamma_8_22 TTCCCCAGGTTATCCC TATGGGGG GAATCGCCTTCCCTCTAATG flavo_1_23 TTCGCTTAGCCACTCAG plancto_4_23 GTCAGTTATGCCCCgamma_8_23 TCCCCAGGTTATCCCC ACACCAAG AGTGAATCGCC CTCTAATGG flavo_1_24AGCTAGTAACCATCGTT plancto_4_24 GTTATGCCCCAGTG gamma_8_24CCCCAGGTTATCCCCC TACCGGCG AATCGCCTTCG TCTAATGGG flavo_1_25GTCAAGCTTGGTACACG plancto_4_25 CTCCACTGGATGTTC gamma_8_25ATCCCCCTCTAATGG TACCAAGG CATTCACCTC GCAGATTCTC flavo_2_1TACAGTACCGTCAGAGC alpha_1_1 CCGGCCCCTTGCGG gamma_9_1 CCTGTCCATCGGTTCCTCTACACG GAAGAAAGCCA CGAAGGCAC flavo_2_2 TCTTACAGTACCGTCAG alpha_1_2CACCTGTGCACCGG gamma_9_2 CTGTCCATCGGTTCCC AGCTCTAC CCCCTTGCGGG GAAGGCACCflavo_2_3 TTACAGTACCGTCAGAG alpha_1_3 GCACCTGTGCACCG gamma_9_3TGTCCATCGGTTCCCG CTCTACAC GCCCCTTGCGG AAGGCACCA flavo_2_4GCATACTCATCTCTTAC alpha_1_4 CTGTGCACCGGCCC gamma_9_4 CAGCACCTGTCCATCCGCCGAAG CTTGCGGGAAG GGTTCCCGAA flavo_2_5 CATACTCATCTCTTACC alpha_1_5ACCTGTGCACCGGC gamma_9_5 AGCACCTGTCCATCG GCCGAAGC CCCTTGCGGGA GTTCCCGAAGflavo_2_6 ACAGTACCGTCAGAGCT alpha_1_6 CCTGTGCACCGGCC gamma_9_6ACCTGTCCATCGGTTC CTACACGT CCTTGCGGGAA CCGAAGGCA flavo_2_7CAGTACCGTCAGAGCTC alpha_1_7 AGCACCTGTGCACC gamma_9_7 GTCCATCGGTTCCCGTACACGTA GGCCCCTTGCG AAGGCACCAA flavo_2_8 CTTACAGTACCGTCAGA alpha_1_8CGGCCCCTTGCGGG gamma_9_8 CACCTGTCCATCGGTT GCTCTACA AAGAAAGCCAT CCCGAAGGCflavo_2_9 TACTCATCTCTTACCGC alpha_1_9 GCACCGGCCCCTTG gamma_9_9CCTCCCTCTCTCGCAC CGAAGCTT CGGGAAGAAAG TCTAGCCTT flavo_2_10ATACTCATCTCTTACCG alpha_1_10 CACCGGCCCCTTGC gamma_9_10 GCACCTGTCCATCGGCCGAAGCT GGGAAGAAAGC TTCCCGAAGG flavo_2_11 CTCATCTCTTACCGCCG alpha_1_11ACCGGCCCCTTGCG gamma_9_11 GCAGCACCTGTCCAT AAGCTTTA GGAAGAAAGCCCGGTTCCCGA flavo_2_12 CGCCCAGTGGCTGCTCT alpha_1_12 TGTGCACCGGCCCCgamma_9_12 ACCTCCCTCTCTCGCA CTGTCTAT TTGCGGGAAGA CTCTAGCCT flavo_2_13CCAGTGGCTGCTCTCTG alpha_1_13 GTGCACCGGCCCCT gamma_9_13 CTCCCTCTCTCGCACTTCTATACC TGCGGGAAGAA CTAGCCTTC flavo_2_14 CCCAGTGGCTGCTCTCT alpha_1_14TGCACCGGCCCCTT gamma_9_14 TCTCTCGCACTCTAGC GTCTATAC GCGGGAAGAAACTTCCAGTA flavo_2_15 TCGCCCAGTGGCTGCTC alpha_1_15 CAGCACCTGTGCACgamma_9_15 TCGCACTCTAGCCTTC TCTGTCTA CGGCCCCTTGC CAGTATCGG flavo_2_16GCCCAGTGGCTGCTCTC alpha_1_16 TTGCGGGAAGAAAG gamma_9_16 CTCGCACTCTAGCCTTTGTCTATA CCATCTCTGGC CCAGTATCG flavo_2_17 GACTCCGATCCGAACTG alpha_1_17GGCCCCTTGCGGGA gamma_9_17 TACCTCCCTCTCTCGC TGATATAG AGAAAGCCATCACTCTAGCC flavo_2_18 AGAACGCATACTCATCT alpha_1_18 CCTTGCGGGAAGAAgamma_9_18 CTCTCGCACTCTAGCC CTTACCGC AGCCATCTCTG TTCCAGTAT flavo_2_19GAACGCATACTCATCTC alpha_1_19 GCAGCACCTGTGCA gamma_9_19 CCCTCTCTCGCACTCTTTACCGCC CCGGCCCCTTG AGCCTTCCA flavo_2_20 CACGTAGAGCGGTTTCT alpha_1_20TGCGGGAAGAAAGC gamma_9_20 TGCAGCACCTGTCCA TCCTGTAT CATCTCTGGCGTCGGTTCCCG flavo_2_21 GTCCTGTCACACTACAT alpha_1_21 AAAGCCATCTCTGGgamma_9_21 ACTCCGTGGTAATCG TTAAGCCC CGATCATACCG CCCTCCCGAA flavo_2_22ACTCATCTCTTACCGCC alpha_1_22 GCCCCTTGCGGGAA gamma_9_22 TCCATCGGTTCCCGAGAAGCTTT GAAAGCCATCT AGGCACCAAT flavo_2_23 CCCCTATCTATCGTAGC alpha_1_23AACAGCAAGCTGCC gamma_9_23 TCACTCCGTGGTAATC CATGGTGT CAACGGCTAGCGCCCTCCCG flavo_2_24 CCCTATCTATCGTAGCC alpha_1_24 CATGCAGCACCTGTgamma_9_24 TCCCTCTCTCGCACTC ATGGTGTG GCACCGGCCCC TAGCCTTCC flavo_2_25CCTATCTATCGTAGCCA alpha_1_25 GCAAGCTGCCCAAC gamma_9_25 CCTCTCTCGCACTCTATGGTGTGC GGCTAGCATCC GCCTTCCAG flavo_3_1 CTGTCACCTAACATTTA alpha_2_1GTGACCCAGAAAGT gamma_10_1 CGCAGGCACATCCGA AGCCCTGG TGCCTTCGCATTAGCGAGAGC flavo_3_2 CCGTCAAGCTTTCTCAC alpha_2_2 GTATTCACCGCGACgamma_10_2 ACGCAGGCACATCCG GAGAAAGT GCGCTGATTCG ATAGCGAGAG flavo_3_3ACCGTCAAGCTTTCTCA alpha_2_3 CGTATTCACCGCGA gamma_10_3 GCGGCTTCGCGGCCCCGAGAAAG CGCGCTGATTC TCTGTACTTG flavo_3_4 CTCTGACTTATTTGTCC alpha_2_4TATTCACCGCGACG gamma_10_4 CGGCTTCGCGGCCCT ACCTACGG CGCTGATTCGCCTGTACTTGC flavo_3_5 CCTCTGACTTATTTGTC alpha_2_5 ACGTATTCACCGCGgamma_10_5 GGCTTCGCGGCCCTCT CACCTACG ACGCGCTGATT GTACTTGCC flavo_3_6GTACCGTCAAGCTTTCT alpha_2_6 GGAACGTATTCACC gamma_10_6 CGCGGCTTCGCGGCCCACGAGAA GCGACGCGCTG CTCTGTACTT flavo_3_7 GAGGCAGATTGTATACG alpha_2_7CCGGGAACGTATTC gamma_10_7 GCTTCGCGGCCCTCTG CGATACTC ACCGCGACGCGTACTTGCCA flavo_3_8 TCTATCGTAGCCTAGGT alpha_2_8 CGGGAACGTATTCAgamma_10_8 CACTACTGGGTAGTTT GTGCCGTT CCGCGACGCGC CCTACGCGT flavo_3_9CCCCTATCTATCGTAGC alpha_2_9 GGGAACGTATTCAC gamma_10_9 CCACTACTGGGTAGTCTAGGTGT CGCGACGCGCT TTCCTACGCG flavo_3_10 ATCTATCGTAGCCTAGG alpha_2_10AACGTATTCACCGC gamma_10_10 CCCCACTACTGGGTA TGTGCCGT GACGCGCTGATGTTTCCTACG flavo_3_11 CCCTATCTATCGTAGCC alpha_2_11 GAACGTATTCACCGgamma_10_11 CCCACTACTGGGTAG TAGGTGTG CGACGCGCTGA TTTCCTACGC flavo_3_12TATCTATCGTAGCCTAG alpha_2_12 CCCGGGAACGTATT gamma_10_12 CCCCCACTACTGGGTGTGTGCCG CACCGCGACGC AGTTTCCTAC flavo_3_13 CCTATCTATCGTAGCCT alpha_2_13ATTCACCGCGACGC gamma_10_13 ACTACCGGGTAGTTT AGGTGTGC GCTGATTCGCGCCTACGCGTT flavo_3_14 CTATCTATCGTAGCCTA alpha_2_14 CCGCGACGCGCTGAgamma_10_14 CACTACCGGGTAGTT GGTGTGCC TTCGCGATTAC TCCTACGCGT flavo_3_15CTATCGTAGCCTAGGTG alpha_2_15 CACCGCGACGCGCT gamma_10_15 ACCGGGTAGTTTCCTTGCCGTTA GATTCGCGATT ACGCGTTACT flavo_3_16 TATCGTAGCCTAGGTGT alpha_2_16CGCGACGCGCTGAT gamma_10_16 CCACTACCGGGTAGT GCCGTTAC TCGCGATTACTTTCCTACGCG flavo_3_17 CTTATTTGTCCACCTAC alpha_2_17 TCACCGCGACGCGCgamma_10_17 CCCCACTACCGGGTA GGACCCTT TGATTCGCGAT GTTTCCTACG flavo_3_18ACTTATTTGTCCACCTA alpha_2_18 ACCGCGACGCGCTG gamma_10_18 CCGGGTAGTTTCCTACCGGACCCT ATTCGCGATTA GCGTTACTC flavo_3_19 GACTTATTTGTCCACCT alpha_2_19GCGACGCGCTGATT gamma_10_19 CCCACTACCGGGTAG ACGGACCC CGCGATTACTATTTCCTACGC flavo_3_20 TGACTTATTTGTCCACC alpha_2_20 TTCACCGCGACGCGgamma_10_20 TACCGGGTAGTTTCCT TACGGACC CTGATTCGCGA ACGCGTTAC flavo_3_21CTGACTTATTTGTCCAC alpha_2_21 TCCTCAGTGTCAGTA gamma_10_21 CCCCCACTACCGGGTCTACGGAC GTGACCCAGA AGTTTCCTAC flavo_3_22 AGATTGTATACGCGATA alpha_2_22CCCAGAAAGTTGCC gamma_10_22 CTACCGGGTAGTTTCC CTCACCCG TTCGCATTTGGTACGCGTTA flavo_3_23 GATTGTATACGCGATAC alpha_2_23 AGTGCGGGCTCATCgamma_10_23 CTGTTGTCCCCCACTA TCACCCGT TTTCGGCGTAT CTGGGTAGT flavo_3_24TCTTCGGGCTATTCCCT alpha_2_24 AAGTGCGGGCTCAT gamma_10_24 CTAGCTAATCTCACGAGTATGAG CTTTCGGCGTA CAGGCACATC flavo_3_25 CTTCGGGCTATTCCCTA alpha_2_25GTGCGGGCTCATCTT gamma_10_25 CAACTAGCTAATCTC GTATGAGG TCGGCGTATAACGCAGGCAC flavo_4_1 CAGGAGATATTCCCATA alpha_3_1 CACCTGTATCCAATCgamma_11_1 GCTTTCCCCCGTAGG CTATGGGG CACCCGAAGT ATATATGCGG flavo_4_2TCAAACTCCCACACGTG alpha_3_2 ACCTGTATCCAATCC gamma_11_2 CTTTCCCCCGTAGGATGGAGTGGT ACCCGAAGTG ATATGCGGT flavo_4_3 CAAACTCCCACACGTGG alpha_3_3CCTGTATCCAATCCA gamma_11_3 TGCTTTCCCCCGTAGG GAGTGGTT CCCGAAGTGAATATATGCG flavo_4_4 GTCAAACTCCCACACGT alpha_3_4 GCACCTGTATCCAAgamma_11_4 CTGCTTTCCCCCGTAG GGGAGTGG TCCACCCGAAG GATATATGC flavo_4_5GGAGATATTCCCATACT alpha_3_5 GGCAGTTCCTTCAA gamma_11_5 CCTGCTTTCCCCCGTAATGGGGCA AGTTCCCACCA GGATATATG flavo_4_6 AGGAGATATTCCCATAC alpha_3_6AGCACCTGTATCCA gamma_11_6 CCCTGCTTTCCCCCGT TATGGGGC ATCCACCCGAAAGGATATAT flavo_4_7 CGTCAAACTCCCACACG alpha_3_7 CGGCAGTTCCTTCAgamma_11_7 CTCACTCAGGCTCATC TGGGAGTG AAGTTCCCACC AAATAGCGC flavo_4_8AAACTCCCACACGTGGG alpha_3_8 CAGCACCTGTATCC gamma_11_8 CCCCTGCTTTCCCCCGAGTGGTTC AATCCACCCGA TAGGATATA flavo_4_9 CTGGGCTATTCCCCTCC alpha_3_9CCGGCAGTTCCTTCA gamma_11_9 GTGTCAGTATCGAGC AAAAGGTA AAGTTCCCACCAGTCAGTCG flavo_4_10 CCGTCAAACTCCCACAC alpha_3_10 GCAGCACCTGTATCgamma_11_10 TCAGTGTCAGTATCG GTGGGAGT CAATCCACCCG AGCCAGTCAG flavo_4_11CTTAACCACTCAGCCCT alpha_3_11 TGCAGCACCTGTAT gamma_11_11 AGTGTCAGTATCGAGTAATCGGG CCAATCCACCC CCAGTCAGTC flavo_4_12 GTTTCCCTGGGCTATTC alpha_3_12TCACCGGCAGTTCCT gamma_11_12 TGTCAGTATCGAGCC CCCTCCAA TCAAAGTTCCAGTCAGTCGC flavo_4_13 GCTTAACCACTCAGCCC alpha_3_13 CTTACAAATCCGCCTgamma_11_13 CAGTGTCAGTATCGA TTAATCGG ACGCTCGCTT GCCAGTCAGT flavo_4_14AACTCCCACACGTGGGA alpha_3_14 ATGCAGCACCTGTA gamma_11_14 CTCAGTGTCAGTATCGTGGTTCT TCCAATCCACC GAGCCAGTCA flavo_4_15 ACCGTCAAACTCCCACA alpha_3_15CGGGCCCATCCAAT gamma_11_15 TCCCCTGCTTTCCCCC CGTGGGAG AGCGCATAAAGGTAGGATAT flavo_4_16 CCACACGTGGGAGTGGT alpha_3_16 GGGCCCATCCAATAgamma_11_16 CCCCACCAACTAGCT TCTTCCTC GCGCATAAAGC AATCTCACTC flavo_4_17AGTTTCCCTGGGCTATT alpha_3_17 GCGGGCCCATCCAA gamma_11_17 CCTCAGTGTCAGTATCCCCCTCCA TAGCGCATAAA GAGCCAGTC flavo_4_18 TTAACCACTCAGCCCTT alpha_3_18ACTTACAAATCCGC gamma_11_18 GTCCCCTGCTTTCCCC AATCGGGC CTACGCTCGCTCGTAGGATA flavo_4_19 CACGTGGGAGTGGTTCT alpha_3_19 CGCGGGCCCATCCAgamma_11_19 TCAGTATCGAGCCAG TCCTCTGT ATAGCGCATAA TCAGTCGCCT flavo_4_20CACACGTGGGAGTGGTT alpha_3_20 GGCCCATCCAATAG gamma_11_20 GTATCGAGCCAGTCACTTCCTCT CGCATAAAGCT GTCGCCTTCG flavo_4_21 ACACGTGGGAGTGGTTC alpha_3_21CACCGGCAGTTCCTT gamma_11_21 AGTATCGAGCCAGTC TTCCTCTG CAAAGTTCCCAGTCGCCTTC flavo_4_22 CGCTTAACCACTCAGCC alpha_3_22 ACCGGCAGTTCCTTCgamma_11_22 TATCGAGCCAGTCAG CTTAATCG AAAGTTCCCA TCGCCTTCGC flavo_4_23ACGTGGGAGTGGTTCTT alpha_3_23 AACTTACAAATCCG gamma_11_23 ATCGAGCCAGTCAGTCCTCTGTA CCTACGCTCGC CGCCTTCGCC flavo_4_24 TTTCCCTGGGCTATTCC alpha_3_24CGCATAAAGCTTTCT gamma_11_24 GTCAGTATCGAGCCA CCTCCAAA CCCGAAGGACGTCAGTCGCC flavo_4_25 TTCCCTGGGCTATTCCC alpha_3_25 CATGCAGCACCTGTgamma_11_25 CAGTATCGAGCCAGT CTCCAAAA ATCCAATCCAC CAGTCGCCTT flavo_5_1CGTCAACAGTTCACACG roseo_1_1 CTCTGGAATCCGCG gamma_12_1 CACTACCTGGTAGATTGAACCTT ACAAGTATGTC TCCTACGCGT flavo_5_2 ACAGTACCGTCAACAGT roseo_1_2TGCCCCTATAAATA gamma_12_2 CCACTACCTGGTAGA TCACACGT GTTGGCGCACCTTCCTACGCG flavo_5_3 CCGTCAACAGTTCACAC roseo_1_3 CCCTATAAATAGTTGgamma_12_3 CCCACTACCTGGTAG GTGAACCT GCGCACCACC ATTCCTACGC flavo_5_4CAGTACCGTCAACAGTT roseo_1_4 CCCCTATAAATAGTT gamma_12_4 AACTGTTGTCCCCCACCACACGTG GGCGCACCAC TACCTGGTA flavo_5_5 TACAGTACCGTCAACAG roseo_1_5GCCCCTATAAATAG gamma_12_5 CAACTGTTGTCCCCCA TTCACACG TTGGCGCACCACTACCTGGT flavo_5_6 ACCGTCAACAGTTCACA roseo_1_6 CGTGGTTGGCTGCCgamma_12_6 CCAACTGTTGTCCCCC CGTGAACC CCTATAAATAG ACTACCTGG flavo_5_7CTACAGTACCGTCAACA roseo_1_7 CTGCCCCTATAAAT gamma_12_7 CCCCACTACCTGGTAGTTCACAC AGTTGGCGCAC GATTCCTACG flavo_5_8 TACCGTCAACAGTTCAC roseo_1_8CCGTGGTTGGCTGC gamma_12_8 CGGTATTGCAACCCT ACGTGAAC CCCTATAAATACTGTACGCCC flavo_5_9 AGTACCGTCAACAGTTC roseo_1_9 TGGCTGCCCCTATAgamma_12_9 ACTGTTGTCCCCCACT ACACGTGA AATAGTTGGCG ACCTGGTAG flavo_5_10GTACCGTCAACAGTTCA roseo_1_10 GGCTGCCCCTATAA gamma_12_10 TCCAACTGTTGTCCCCCACGTGAA ATAGTTGGCGC CACTACCTG flavo_5_11 CCTACAGTACCGTCAAC roseo_1_11GGAATCCGCGACAA gamma_12_11 CCCCCACTACCTGGT AGTTCACA GTATGTCAAGGAGATTCCTAC flavo_5_12 TCCTACAGTACCGTCAA roseo_1_12 GCTGCCCCTATAAAgamma_12_12 GCGGTATTGCAACCC CAGTTCAC TAGTTGGCGCA TCTGTACGCC flavo_5_13CCGAAGAAAAAGATGT roseo_1_13 ACCGTGGTTGGCTG gamma_12_13 GCGGTATCGCAACCCTTCCACCCC CCCCTATAAAT TCTGTACGTT flavo_5_14 CTCAGACCGCAATTAGT roseo_1_14CCATCTCTGGAATCC gamma_12_14 TCTATCAGTTTGGGGT CCGAACAG GCGACAAGTAGCAGTTCCC flavo_5_15 TAGCCACTCAGACCGCA roseo_1_15 ATAGTTGGCGCACCgamma_12_15 GTCTATCAGTTTGGG ATTAGTCC ACCTTCGGGTA GTGCAGTTCC flavo_5_16TTAGCCACTCAGACCGC roseo_1_16 GGAATCCATCTCTG gamma_12_16 CTGTTGTCCCCCACTAAATTAGTC GAATCCGCGAC CCTGGTAGA flavo_5_17 ACTCAGACCGCAATTAG roseo_1_17TACCGTGGTTGGCTG gamma_12_17 CTATCAGTTTGGGGT TCCGAACA CCCCTATAAAGCAGTTCCCA flavo_5_18 AGATGTTTCCACCCCTG roseo_1_18 GAATCCGCGACAAGgamma_12_18 CTGTTGCTAACGTCAC TCAAACTG TATGTCAAGGG AGCTAAGGG flavo_5_19CAGACCGCAATTAGTCC roseo_1_19 TCCATCTCTGGAATC gamma_12_19 CAGTTTGGGGTGCAGGAACAGCT CGCGACAAGT TTCCCAGGTT flavo_5_20 GCCACTCAGACCGCAAT roseo_1_20ATCCATCTCTGGAAT gamma_12_20 AGTTTGGGGTGCAGT TAGTCCGA CCGCGACAAGTCCCAGGTTG flavo_5_21 CACTCAGACCGCAATTA roseo_1_21 TAGTTGGCGCACCAgamma_12_21 TTCCAACTGTTGTCCC GTCCGAAC CCTTCGGGTAG CCACTACCT flavo_5_22CTTAGCCACTCAGACCG roseo_1_22 CCTACCGTGGTTGG gamma_12_22 TATCAGTTTGGGGTGCAATTAGT CTGCCCCTATA CAGTTCCCAG flavo_5_23 AGCCACTCAGACCGCA roseo_1_23CTACCGTGGTTGGCT gamma_12_23 CGGTATCGCAACCCT ATTAGTCCG GCCCCTATAACTGTACGTTC flavo_5_24 TCAGACCGCAATTAGTC roseo_1_24 ACGTCGTCCACACCgamma_12_24 CCCCACCAACTAACT CGAACAGC TTCCTCCGGCT AATCTCACGC flavo_5_25ACTTTCGCTTAGCCACT roseo_1_25 GACGTCGTCCACAC gamma_12_25 GTCAGCGACTAGCAACAGACCGC CTTCCTCCGGC GCTAGTCCTG flavo_6_1 AGTGCCGGAGTTAAGCC roseo_2_1GTCACCGGGTCACC gamma_13_1 CGCCACTGAAAGACA CCTGCATT GAAGTGAAAACTTGTCTCCCA flavo_6_2 GTGCCGGAGTTAAGCCC roseo_2_2 ACCGGGTCACCGAAgamma_13_2 GCGCCACTGAAAGAC CTGCATTT GTGAAAACCAG ATTGTCTCCC flavo_6_3CAGTGCCGGAGTTAAGC roseo_2_3 CACCGGGTCACCGA gamma_13_3 TGCGCCACTGAAAGACCCTGCAT AGTGAAAACCA CATTGTCTCC flavo_6_4 TGCCGGAGTTAAGCCCC roseo_2_4TCACCGGGTCACCG gamma_13_4 TGTCAGTACAGATCC TGCATTTC AAGTGAAAACCAGGAGGCCGC flavo_6_5 AGTTAAGCCCCTGCATT roseo_2_5 TGTCACCGGGTCACgamma_13_5 GTGTCAGTACAGATC TCACCACT CGAAGTGAAAA CAGGAGGCCG flavo_6_6GCAGTGCCGGAGTTAA roseo_2_6 CCGGGTCACCGAAG gamma_13_6 CTGCGCCACTGAAAGGCCCCTGCA TGAAAACCAGA ACATTGTCTC flavo_6_7 GTTAAGCCCCTGCATTT roseo_2_7AGATCTCTCTGGCG gamma_13_7 CTTGGCTCCAAAAGG CACCACTG GTCCCGGGATGCACACTCTCA flavo_6_8 GGCAGTGCCGGAGTTA roseo_2_8 ACCAGATCTCTCTGgamma_13_8 GAGAGCTTCAAGAGA AGCCCCTGC GCGGTCCCGGG GGCCCTCTTT flavo_6_9TGGCAGTGCCGGAGTTA roseo_2_9 AACCAGATCTCTCT gamma_13_9 CGAGAGCTTCAAGAGAGCCCCTG GGCGGTCCCGG AGGCCCTCTT flavo_6_10 GAGTTAAGCCCCTGCAT roseo_2_10AAACCAGATCTCTC gamma_13_10 GCGAGAGCTTCAAGA TTCACCAC TGGCGGTCCCGGAGGCCCTCT flavo_6_11 GCCGGAGTTAAGCCCCT roseo_2_11 TCTCTGGCGGTCCCGgamma_13_11 TAGCGAGAGCTTCAA GCATTTCA GGATGTCAAG GAGAGGCCCT flavo_6_12ATGGCAGTGCCGGAGTT roseo_2_12 ATCTCTCTGGCGGTC gamma_13_12 AGAGCTTCAAGAGAGAAGCCCCT CCGGGATGTC GCCCTCTTTC flavo_6_13 TTAAGCCCCTGCATTTC roseo_2_13GATCTCTCTGGCGGT gamma_13_13 AGCGAGAGCTTCAAG ACCACTGA CCCGGGATGTAGAGGCCCTC flavo_6_14 GGAGTTAAGCCCCTGCA roseo_2_14 CAGATCTCTCTGGCgamma_13_14 GTCAGTACAGATCCA TTTCACCA GGTCCCGGGAT GGAGGCCGCC flavo_6_15CGGAGTTAAGCCCCTGC roseo_2_15 TCTGGCGGTCCCGG gamma_13_15 TCAGTACAGATCCAGATTTCACC GATGTCAAGGG GAGGCCGCCT flavo_6_16 CCCTGCATTTCACCACT roseo_2_16CTCTGGCGGTCCCG gamma_13_16 CAGTACAGATCCAGG GACTTATC GGATGTCAAGGAGGCCGCCTT flavo_6_17 CAATGGCAGTGCCGGA roseo_2_17 CCAGATCTCTCTGGCgamma_13_17 AGTACAGATCCAGGA GTTAAGCCC GGTCCCGGGA GGCCGCCTTC flavo_6_18TCAATGGCAGTGCCGGA roseo_2_18 TCTCTCTGGCGGTCC gamma_13_18 GCTGCGCCACTGAAAGTTAAGCC CGGGATGTCA GACATTGTCT flavo_6_19 CCTTACGGTCACCGACT roseo_2_19CTCTCTGGCGGTCCC gamma_13_19 GAGCTTCAAGAGAGG TCAGGCAC GGGATGTCAACCCTCTTTCT flavo_6_20 CCGGAGTTAAGCCCCTG roseo_2_20 CTGGCGGTCCCGGGgamma_13_20 TCTTGGCTCCAAAAG CATTTCAC ATGTCAAGGGT GCACACTCTC flavo_6_21AATGGCAGTGCCGGAG roseo_2_21 ACCTGTCACCGGGT gamma_13_21 AGTGTCAGTACAGATTTAAGCCCC CACCGAAGTGA CCAGGAGGCC flavo_6_22 TATCAATGGCAGTGCCG roseo_2_22CCTGTCACCGGGTC gamma_13_22 GGCCCTCTTTCTCCCT GAGTTAAG ACCGAAGTGAATAGGAGGTA flavo_6_23 GTATCAATGGCAGTGCC roseo_2_23 CTGTCACCGGGTCAgamma_13_23 AGCTTCAAGAGAGGC GGAGTTAA CCGAAGTGAAA CCTCTTTCTC flavo_6_24CCCCTGCATTTCACCAC roseo_2_24 CGGGTCACCGAAGT gamma_13_24 AGCTGCGCCACTGAATGACTTAT GAAAACCAGAT AGACATTGTC flavo_6_25 TAAGCCCCTGCATTTCA roseo_2_25AAAACCAGATCTCT gamma_13_25 CGAGAGCATCAAGAG CCACTGAC CTGGCGGTCCCAGGCCCTCTT flavo_7_1 TCTTACAGTACCGTCAC roseo_3_1 GCCGCTACACCCGAgamma_14_1 GGCGGTCAACTTACT CAGACTAC AGGTGCCGCTC ACGTTAGCTG flavo_7_2CTTACAGTACCGTCACC roseo_3_2 CTACACCCGAAGGT gamma_14_2 CCAGGCGGTCAACTTAGACTACA GCCGCTCGACT ACTACGTTAG flavo_7_3 CGTCACCAGACTACACG roseo_3_3GCTACACCCGAAGG gamma_14_3 GCGGTCAACTTACTA TAGTCCTT TGCCGCTCGACCGTTAGCTGC flavo_7_4 GTACCGTCACCAGACTA roseo_3_4 CCGCTACACCCGAAgamma_14_4 CAGGCGGTCAACTTA CACGTAGT GGTGCCGCTCG CTACGTTAGC flavo_7_5CCGTCACCAGACTACAC roseo_3_5 CGCTACACCCGAAG gamma_14_5 CCCAGGCGGTCAACTGTAGTCCT GTGCCGCTCGA TACTACGTTA flavo_7_6 TACCGTCACCAGACTAC roseo_3_6CGCCGCTACACCCG gamma_14_6 CCGAGGGCACTGCTT ACGTAGTC AAGGTGCCGCTCATTACAAAG flavo_7_7 ACCGTCACCAGACTACA roseo_3_7 CCGCCGCTACACCCgamma_14_7 CGAGGGCACTGCTTC CGTAGTCC GAAGGTGCCGC ATTACAAAGC flavo_7_8TTACAGTACCGTCACCA roseo_3_8 TACACCCGAAGGTG gamma_14_8 TCCCGAGGGCACTGCGACTACAC CCGCTCGACTT TTCATTACAA flavo_7_9 GTCACCAGACTACACGT roseo_3_9TCCGCCGCTACACC gamma_14_9 CCCGAGGGCACTGCT AGTCCTTA CGAAGGTGCCGTCATTACAAA flavo_7_10 TACAGTACCGTCACCAG roseo_3_10 ACACCCGAAGGTGCgamma_14_10 CCCCAGGCGGTCAAC ACTACACG CGCTCGACTTG TTACTACGTT flavo_7_11ACAGTACCGTCACCAGA roseo_3_11 GTCCGCCGCTACAC gamma_14_11 TCCCCAGGCGGTCAACTACACGT CCGAAGGTGCC CTTACTACGT flavo_7_12 AACTTTCACCCCTGACT roseo_3_12ACCCGAAGGTGCCG gamma_14_12 CTCCCGAGGGCACTG TAACAGCC CTCGACTTGCACTTCATTACA flavo_7_13 CAGTACCGTCACCAGAC roseo_3_13 CACCCGAAGGTGCCgamma_14_13 CTCCCCAGGCGGTCA TACACGTA GCTCGACTTGC ACTTACTACG flavo_7_14CCGGTCGTCAGCAAGA roseo_3_14 CGTCCGCCGCTACA gamma_14_14 GCTCCCGAGGGCACTGCAAGCTCC CCCGAAGGTGC GCTTCATTAC flavo_7_15 ACTTTCACCCCTGACTT roseo_3_15CACCTGGTCTCTTAC gamma_14_15 TCTTGGCTCCCGAGG AACAGCCC GAGAAAACCGGCACTGCTTC flavo_7_16 CCCTGACTTAACAGCCC roseo_3_16 CCAGGAGTTTTGGAgamma_14_16 GGCTCCCGAGGGCAC GCCTACGG GGCCGTTCCAG TGCTTCATTA flavo_7_17TCGCTTGGCCGCTCAGA roseo_3_17 ACCTGGTCTCTTACG gamma_14_17TATCTTGGCTCCCGAG TCGAAATC AGAAAACCGG GGCACTGCT flavo_7_18CGCTTGGCCGCTCAGAT roseo_3_18 CCGGATCTCTCCGG gamma_14_18 ACTCCCCAGGCGGTCCGAAATCC CGGTCCAGGGA AACTTACTAC flavo_7_19 TTCGCTTGGCCGCTCAG roseo_3_19CCCGAAGGTGCCGC gamma_14_19 ATCTTGGCTCCCGAG ATCGAAAT TCGACTTGCATGGCACTGCTT flavo_7_20 TTTCGCTTGGCCGCTCA roseo_3_20 ACCAGGAGTTTTGGgamma_14_20 TACTACGTTAGCTGC GATCGAAA AGGCCGTTCCA GCCACTGAGA flavo_7_21GCTTGGCCGCTCAGATC roseo_3_21 CAGGAGTTTTGGAG gamma_14_21 GTATCTTGGCTCCCGAGAAATCCA GCCGTTCCAGG GGGCACTGC flavo_7_22 CTTGGCCGCTCAGATCG roseo_3_22CCGAAGGTGCCGCT gamma_14_22 CTTGGCTCCCGAGGG AAATCCAA CGACTTGCATGCACTGCTTCA flavo_7_23 TTGGCCGCTCAGATCGA roseo_3_23 CCGTCCGCCGCTACgamma_14_23 TGGCTCCCGAGGGCA AATCCAAA ACCCGAAGGTG CTGCTTCATT flavo_7_24GGCTATCCCTTAGTGTA roseo_3_24 AAACCGGATCTCTC gamma_14_24 ACTACGTTAGCTGCGAGGCAGAT CGGCGGTCCAG CCACTGAGAA flavo_7_25 GGGCTATCCCTTAGTGT roseo_3_25CCTGGTCTCTTACGA gamma_14_25 TTGGCTCCCGAGGGC AAGGCAGA GAAAACCGGAACTGCTTCAT flavo_8_1 GCCGAAATACGGTACTA roseo_4_1 CGTACCATCTCTGGTgamma_15_1 TCCGTAGAAGTCCGG CGGGGCAT AGTAGCACAG GCCGTGTCTC flavo_8_2GATGCCGAAATACGGT roseo_4_2 CCATCTCTGGTAGTA gamma_15_2 CCGTAGAAGTCCGGGACTACGGGG GCACAGGATG CCGTGTCTCA flavo_8_3 ATGCCGAAATACGGTAC roseo_4_3GTACCATCTCTGGTA gamma_15_3 CGTAGAAGTCCGGGC TACGGGGC GTAGCACAGGCGTGTCTCAG flavo_8_4 TGCCGAAATACGGTACT roseo_4_4 CTGGTAGTAGCACAgamma_15_4 GTAGAAGTCCGGGCC ACGGGGCA GGATGTCAAGG GTGTCTCAGT flavo_8_5ACCGTATAACGATGCCG roseo_4_5 TGGTAGTAGCACAG gamma_15_5 TTCCGTAGAAGTCCGAAATACGG GATGTCAAGGG GGCCGTGTCT flavo_8_6 CCGTATAACGATGCCGA roseo_4_6GAAGGGAACGTACC gamma_15_6 CTTCCGTAGAAGTCC AATACGGT ATCTCTGGTAGGGGCCGTGTC flavo_8_7 CGATGCCGAAATACGGT roseo_4_7 CCTTAGAGAAGGGCgamma_15_7 TAGAAGTCCGGGCCG ACTACGGG ATATTCCCACG TGTCTCAGTC flavo_8_8CCGAAATACGGTACTAC roseo_4_8 GGTAGTAGCACAGG gamma_15_8 ACTGCTGCCTTCCGTAGGGGCATT ATGTCAAGGGT GAAGTCCGG flavo_8_9 ACGATGCCGAAATACG roseo_4_9GGGAACGTACCATC gamma_15_9 CATGCAGTCGAGTTC GTACTACGG TCTGGTAGTAGCAGACTGCAA flavo_8_10 AACGATGCCGAAATAC roseo_4_10 GGAACGTACCATCTgamma_15_10 CCTCGAGCTATCCCCC GGTACTACG CTGGTAGTAGC TCCATTGGG flavo_8_11CGAAGGAAAAGTCATC roseo_4_11 CGAAGGGAACGTAC gamma_15_11 AGAAGTCCGGGCCGTTCTGACCCT CATCTCTGGTA GTCTCAGTCC flavo_8_12 CGAAATACGGTACTACG roseo_4_12CCGAAGGGAACGTA gamma_15_12 TCCTCGAGCTATCCCC GGGCATTA CCATCTCTGGTCTCCATTGG flavo_8_13 CCGAAGGAAAAGTCAT roseo_4_13 CGTCCCCGAAGGGAgamma_15_13 CTCGAGCTATCCCCCT CTCTGACCC ACGTACCATCT CCATTGGGT flavo_8_14GTCATCTCTGACCCTGT roseo_4_14 CCCCGAAGGGAACG gamma_15_14 TCATGCAGTCGAGTTCAATATGC TACCATCTCTG CCAGACTGCA flavo_8_15 CCCGAAGGAAAAGTCA roseo_4_15GTCCCCGAAGGGAA gamma_15_15 CCTTCCGTAGAAGTC TCTCTGACC CGTACCATCTCCGGGCCGTGT flavo_8_16 TACAAGGCAGGTTCCAT roseo_4_16 GCGTCCCCGAAGGGgamma_15_16 GCGCCACTGGATAAA ACGCGGTG AACGTACCATC TCCAACGGCT flavo_8_17GGCTTTAACCGTATAAC roseo_4_17 ACTGCGTCCCCGAA gamma_15_17 TGCGCCACTGGATAAGATGCCGA GGGAACGTACC ATCCAACGGC flavo_8_18 CTGGGCTATTCCCCTGT roseo_4_18CTGCGTCCCCGAAG gamma_15_18 TTCCTCGAGCTATCCC ACAAGGCA GGAACGTACCACCTCCATTG flavo_8_19 GAAGGAAAAGTCATCT roseo_4_19 CCCGAAGGGAACGTgamma_15_19 GTTCCAGACTGCAAT CTGACCCTG ACCATCTCTGG TCGGACTACG flavo_8_20GCCCGAAGGAAAAGTC roseo_4_20 TGCGTCCCCGAAGG gamma_15_20 CCAGCTCGCGCTTTGATCTCTGAC GAACGTACCAT GCAACCGTTT flavo_8_21 GTACAAGGCAGGTTCCA roseo_4_21CTTAGAGAAGGGCA gamma_15_21 TCGAGCTATCCCCCTC TACGCGGT TATTCCCACGCCATTGGGTA flavo_8_22 TGTACAAGGCAGGTTCC roseo_4_22 GAAGGGCGCGCTCGgamma_15_22 GCTGCGCCACTGGAT ATACGCGG ACTTGCATGTA AAATCCAACG flavo_8_23CCTGGGCTATTCCCCTG roseo_4_23 CACTGCGTCCCCGA gamma_15_23 CGCCACTGGATAAATTACAAGGC AGGGAACGTAC CCAACGGCTA flavo_8_24 ACAAGGCAGGTTCCATA roseo_4_24TCACTGCGTCCCCG gamma_15_24 CTGCGCCACTGGATA CGCGGTGC AAGGGAACGTAAATCCAACGG flavo_8_25 GGCAGGTTCCATACGCG roseo_4_25 TCCCCGAAGGGAACgamma_15_25 TTTCCTCGAGCTATCC GTGCGCAC GTACCATCTCT CCCTCCATT flavo_9_1ATTCCGCCTACTTCAAT roseo_5_1 GTCACTATGTCCCG gamma_16_1 TTTAAGGGTTTGGCTCACAACTCA AAGGAAAGCCT CAGCTCGCG flavo_9_2 TTCCGCCTACTTCAATA roseo_5_2CCGAAGGAAAGCCT gamma_16_2 TTTTAAGGGTTTGGCT CAACTCAA GATCTCTCAGGCCAGCTCGC flavo_9_3 TATTCCGCCTACTTCAA roseo_5_3 TGTCACTATGTCCCGgamma_16_3 TTAAGGGTTTGGCTCC TACAACTC AAGGAAAGCC AGCTCGCGC flavo_9_4TCCGCCTACTTCAATAC roseo_5_4 TCCCGAAGGAAAGC gamma_16_4 GTTTTAAGGGTTTGGCAACTCAAG CTGATCTCTCA TCCAGCTCG flavo_9_5 CATATTCCGCCTACTTC roseo_5_5TCACTATGTCCCGA gamma_16_5 CACGCGGTATACCTG AATACAAC AGGAAAGCCTGGATCAGGGTT flavo_9_6 CCGCCTACTTCAATACA roseo_5_6 CCCGAAGGAAAGCCgamma_16_6 ACACGCGGTATACCT ACTCAAGA TGATCTCTCAG GGATCAGGGT flavo_9_7CGCCTACTTCAATACAA roseo_5_7 CTGTCACTATGTCCC gamma_16_7 CTTCCTCCGGGTTTCACTCAAGAT GAAGGAAAGC CCCGGCAGT flavo_9_8 GAACTCAAGGTCCCGA roseo_5_8GTCCCGAAGGAAAG gamma_16_8 TCCTCCGGGTTTCACC ACAGCTAGT CCTGATCTCTCCGGCAGTCT flavo_9_9 TCAGAACTCAAGGTCCC roseo_5_9 GCCTGATCTCTCAGgamma_16_9 CTTCACACACGCGGT GAACAGCT GTTGTCATAGG ATACCTGGAT flavo_9_10ACTCAAGGTCCCGAACA roseo_5_10 TGACTGACTAATCC gamma_16_10 CACACGCGGTATACCGCTAGTAT GCCTACGTACG TGGATCAGGG flavo_9_11 GATGCCTATCAATAATA roseo_5_11CTGACTGACTAATC gamma_16_11 ACACACGCGGTATAC CCATGAGG CGCCTACGTACCTGGATCAGG flavo_9_12 AGAACTCAAGGTCCCG roseo_5_12 CGAAGGAAAGCCTGgamma_16_12 CACACACGCGGTATA AACAGCTAG ATCTCTCAGGT CCTGGATCAG flavo_9_13CTCAAGGTCCCGAACAG roseo_5_13 CACTATGTCCCGAA gamma_16_13 CCTTCCTCCGGGTTTCCTAGTATC GGAAAGCCTGA ACCCGGCAG flavo_9_14 AACTCAAGGTCCCGAAC roseo_5_14GCACCTGTCACTAT gamma_16_14 TTCCTCCGGGTTTCAC AGCTAGTA GTCCCGAAGGACCGGCAGTC flavo_9_15 CAGAACTCAAGGTCCCG roseo_5_15 CCTGTCACTATGTCCgamma_16_15 CCTCCGGGTTTCACCC AACAGCTA CGAAGGAAAG GGCAGTCTC flavo_9_16CTCAGAACTCAAGGTCC roseo_5_16 CTATGTCCCGAAGG gamma_16_16 TTCACACACGCGGTACGAACAGC AAAGCCTGATC TACCTGGATC flavo_9_17 TCAAGGTCCCGAACAGC roseo_5_17ATGTCCCGAAGGAA gamma_16_17 CGCCTTCCTCCGGGTT TAGTATCC AGCCTGATCTCTCACCCGGC flavo_9_18 GCTCAGAACTCAAGGTC roseo_5_18 AGCACCTGTCACTAgamma_16_18 CTCCGGGTTTCACCCG CCGAACAG TGTCCCGAAGG GCAGTCTCC flavo_9_19CTACATATTCCGCCTAC roseo_5_19 CAGCACCTGTCACT gamma_16_19 GCGGTATACCTGGATTTCAATAC ATGTCCCGAAG CAGGGTTGCC flavo_9_20 GCCTACTTCAATACAAC roseo_5_20CCTCCGAAGAGGTT gamma_16_20 CGGTATACCTGGATC TCAAGATG AGCGCACGGCCAGGGTTGCCC flavo_9_21 TACACGTAAGGCTTATT roseo_5_21 TCCGCTGCCTCCTCCgamma_16_21 GGTATACCTGGATCA CTTCCTGT GAAGAGGTTA GGGTTGCCCC flavo_9_22CACGTAAGGCTTATTCT roseo_5_22 CCGCTGCCTCCTCCG gamma_16_22 TCTTCACACACGCGGTCCTGTAT AAGAGGTTAG TATACCTGGA flavo_9_23 ACACGTAAGGCTTATTC roseo_5_23TGTCCCGAAGGAAA gamma_16_23 TCACACACGCGGTAT TTCCTGTA GCCTGATCTCTACCTGGATCA flavo_9_24 CTTAGCCGCTCAGAACT roseo_5_24 CACCTGTCACTATGTgamma_16_24 GCCTTCCTCCGGGTTT CAAGGTCC CCCGAAGGAA CACCCGGCA flavo_9_25CGCTCAGAACTCAAGGT roseo_5_25 GCAGCACCTGTCAC gamma_16_25 CGCGGTATACCTGGACCCGAACA TATGTCCCGAA TCAGGGTTGC flavo_10_1 CGCTTAGCCACTCATCT roseo_6_1CGATAAAACCTAGT gamma_17_1 GGCTCCTCCAATAGT AACCAATG CTCCTAGGCGGGACCGGTCCG flavo_10_2 CTTTCGCTTAGCCACTC roseo_6_2 CCGAGGCTATTCCGgamma_17_2 AGGCTCCTCCAATAG ATCTAACC AAGCAAAAGGT TGACCGGTCC flavo_10_3ACACGTCGGAGTGTTTC roseo_6_3 CCCGAGGCTATTCC gamma_17_3 CAGGCTCCTCCAATATTCCTGTA GAAGCAAAAGG GTGACCGGTC flavo_10_4 CCCGTGCGCCACTCGTC roseo_6_4AAAACCTAGTCTCC gamma_17_4 CATGTATTAGGCCTG ATCTGGTG TAGGCGGTCAGCCGCCAACGT flavo_10_5 ACCCGTGCGCCACTCGT roseo_6_5 AAACCTAGTCTCCTgamma_17_5 GCTCCTCCAATAGTG CATCTGGT AGGCGGTCAGA ACCGGTCCGA flavo_10_6CACCCGTGCGCCACTCG roseo_6_6 TCCCGAGGCTATTCC gamma_17_6 GCAGGCTCCTCCAATTCATCTGG GAAGCAAAAG AGTGACCGGT flavo_10_7 TACAACCCGTAGGGCTT roseo_6_7CTAGTCTCCTAGGC gamma_17_7 CGCCTGAGAGCAAGC TCATCCTG GGTCAGAGGATTCCCATCGTT flavo_10_8 ACAACCCGTAGGGCTTT roseo_6_8 AACCTAGTCTCCTAgamma_17_8 ACGCCTGAGAGCAAG CATCCTGC GGCGGTCAGAG CTCCCATCGT flavo_10_9AACCCGTAGGGCTTTCA roseo_6_9 CCTAGTCTCCTAGGC gamma_17_9 GCCTGAGAGCAAGCTTCCTGCAC GGTCAGAGGA CCCATCGTTT flavo_10_10 CAGTTTACAACCCGTAG roseo_6_10TAGTCTCCTAGGCG gamma_17_10 GACGCCTGAGAGCAA GGCTTTCA GTCAGAGGATGGCTCCCATCG flavo_10_11 CAACCCGTAGGGCTTTC roseo_6_11 CCTCTCAAACCAGCgamma_17_11 AATCCTACGCAGGCT ATCCTGCA TACTGATCGCA CCTCCAATAG flavo_10_12TTACAACCCGTAGGGCT roseo_6_12 TCCTCTCAAACCAG gamma_17_12 GCATGTATTAGGCCTTTCATCCT CTACTGATCGC GCCGCCAACG flavo_10_13 AGCAGTTTACAACCCGT roseo_6_13CTCTCAAACCAGCT gamma_17_13 CTAATCCTACGCAGG AGGGCTTT ACTGATCGCAGCTCCTCCAAT flavo_10_14 GCAGTTTACAACCCGTA roseo_6_14 CTCAAACCAGCTACgamma_17_14 GCTAATCCTACGCAG GGGCTTTC TGATCGCAGAC GCTCCTCCAA flavo_10_15AAGCAGTTTACAACCCG roseo_6_15 CAGCTACTGATCGC gamma_17_15 CGACGCCTGAGAGCATAGGGCTT AGACTTGGTAG AGCTCCCATC flavo_10_16 CACGTCGGAGTGTTTCT roseo_6_16CCAGCTACTGATCG gamma_17_16 CCTGAGAGCAAGCTC TCCTGTAT CAGACTTGGTACCATCGTTTC flavo_10_17 TGCGCCACTCGTCATCT roseo_6_17 CCATGCAGCACCTGgamma_17_17 CTCCTCCAATAGTGA GGTGCAAG TCACTCTGTAT CCGGTCCGAA flavo_10_18CCGTGCGCCACTCGTCA roseo_6_18 CATGCAGCACCTGT gamma_17_18 ATCCTACGCAGGCTCTCTGGTGC CACTCTGTATC CTCCAATAGT flavo_10_19 GCGCCACTCGTCATCTG roseo_6_19AACCAGCTACTGAT gamma_17_19 CGCAGGCTCCTCCAA GTGCAAGC CGCAGACTTGGTAGTGACCGG flavo_10_20 CGTGCGCCACTCGTCAT roseo_6_20 ACCAGCTACTGATCgamma_17_20 AGCTAATCCTACGCA CTGGTGCA GCAGACTTGGT GGCTCCTCCA flavo_10_21GTGCGCCACTCGTCATC roseo_6_21 GCCATGCAGCACCT gamma_17_21 TCGACGCCTGAGAGCTGGTGCAA GTCACTCTGTA AAGCTCCCAT flavo_10_22 GTTTACAACCCGTAGGG roseo_6_22AGTTTCCCGAGGCT gamma_17_22 CTGAGAGCAAGCTCC CTTTCATC ATTCCGAAGCACATCGTTTCC flavo_10_23 TTTACAACCCGTAGGGC roseo_6_23 GTTTCCCGAGGCTATgamma_17_23 TGTATTAGGCCTGCC TTTCATCC TCCGAAGCAA GCCAACGTTC flavo_10_24GCACCCGTGCGCCACTC roseo_6_24 GGCGGTCAGAGGAT gamma_17_24 TGCATGTATTAGGCCTGTCATCTG GTCAAGGGTTG GCCGCCAAC flavo_10_25 GCGAAGTGGCTGCTCTC roseo_6_25AGGCGGTCAGAGGA gamma_17_25 CGCCACCGGTATTCCT TGTACCGG TGTCAAGGGTTCAGAATATC flavo_11_1 GTACAAGTACTTTATGC alpha_4_1 CGACAGGCATGCCTgamma_19_1 GAGGTTGCGACCCTT TGCCCCTC GCCAACAACTA TGTCCTTCCC flavo_11_2CCGCCGGAGCTTTTCTT alpha_4_2 CCGACAGGCATGCC gamma_19_2 GCGAGGTTGCGACCCAAAAACTC TGCCAACAACT TTTGTCCTTC flavo_11_3 CGGTCGCCATCAAAGTA alpha_4_3ACCGACAGGCATGC gamma_19_3 CGAAACCTTTCAAGA CAAGTACT CTGCCAACAACAGAGGGCTCC flavo_11_4 CCGGTCGCCATCAAAGT alpha_4_4 GACAGGCATGCCTGgamma_19_4 AAAGTGGTGAGCGCC ACAAGTAC CCAACAACTAG CAGATAAGCT flavo_11_5CGTCCCTCAGCGTCAGT alpha_4_5 CCGTCTGCCACTATA gamma_19_5 TGAGCGCCCAGATAATAATTGTT TCGTTCGACT GCTACCCACT flavo_11_6 TACAAGTACTTTATGCT alpha_4_6CACCGACAGGCATG gamma_19_6 CAAAGTGGTGAGCGC GCCCCTCG CCTGCCAACAACCAGATAAGC flavo_11_7 CACGCGGCATCGCTGGA alpha_4_7 CCCGTCTGCCACTATgamma_19_7 GTGGTGAGCGCCCAG TCAGAGTT ATCGTTCGAC ATAAGCTACC flavo_11_8TCGTCCCTCAGCGTCAG alpha_4_8 CAGGCATGCCTGCC gamma_19_8 AGTGGTGAGCGCCCATTAATTGT AACAACTAGCT GATAAGCTAC flavo_11_9 TCACGCGGCATCGCTGG alpha_4_9ACAGGCATGCCTGC gamma_19_9 GTGAGCGCCCAGATA ATCAGAGT CAACAACTAGCAGCTACCCAC flavo_11_10 TGCCAGTATCAAAGGCA alpha_4_10 TCACCGACAGGCATgamma_19_10 GGTGAGCGCCCAGAT GTTCTACC GCCTGCCAACA AAGCTACCCA flavo_11_11ACAAGTACTTTATGCTG alpha_4_11 GCATGCCTGCCAAC gamma_19_11 TGGTGAGCGCCCAGACCCCTCGA AACTAGCTCTC TAAGCTACCC flavo_11_12 GTACATCGAACAGCTAG alpha_4_12GGCATGCCTGCCAA gamma_19_12 AAGTGGTGAGCGCCC TGACCATC CAACTAGCTCTAGATAAGCTA flavo_11_13 GCCAGTATCAAAGGCA alpha_4_13 CACCCGTCTGCCACTgamma_19_13 CGCCCAGATAAGCTA GTTCTACCG ATATCGTTCG CCCACTTCTT flavo_11_14TTCGTCCCTCAGCGTCA alpha_4_14 ACCCGTCTGCCACT gamma_19_14 GCGCCCAGATAAGCTGTTAATTG ATATCGTTCGA ACCCACTTCT flavo_11_15 CAAGTACTTTATGCTGC alpha_4_15GTCACCGACAGGCA gamma_19_15 GCGAAACCTTTCAAG CCCTCGAC TGCCTGCCAACAAGAGGGCTC flavo_11_16 CGCCGGTCGCCATCAAA alpha_4_16 AGGCATGCCTGCCAgamma_19_16 AGCGCCCAGATAAGC GTACAAGT ACAACTAGCTC TACCCACTTC flavo_11_17TCGCCGGTCGCCATCAA alpha_4_17 CTCACCCGTCTGCCA gamma_19_17 ACAAAGTGGTGAGCGAGTACAAG CTATATCGTT CCCAGATAAG flavo_11_18 GCCGGTCGCCATCAAAG alpha_4_18TCACCCGTCTGCCAC gamma_19_18 CACAAAGTGGTGAGC TACAAGTA TATATCGTTCGCCCAGATAA flavo_11_19 TTCGCCGGTCGCCATCA alpha_4_19 CATGCCTGCCAACAgamma_19_19 CGAGGTTGCGACCCT AAGTACAA ACTAGCTCTCA TTGTCCTTCC flavo_11_20CGTTCGCCGGTCGCCAT alpha_4_20 CCTGCCAACAACTA gamma_19_20 GAGCGCCCAGATAAGCAAAGTAC GCTCTCATCGT CTACCCACTT flavo_11_21 GTTCGCCGGTCGCCATC alpha_4_21CGTCACCGACAGGC gamma_19_21 CGCGAGGTTGCGACC AAAGTACA ATGCCTGCCAACTTTGTCCTT flavo_11_22 TACCTATCGGAGCTTAG alpha_4_22 CTCGGTATTCCGCTAgamma_19_22 GACGCCTAAGAGCAA GTGAGCCG ACCTCTCCTG GCTCTTATCG flavo_11_23TATCGGAGCTTAGGTGA alpha_4_23 ACTCACCCGTCTGCC gamma_19_23 TCACAAAGTGGTGAGGCCGTTAC ACTATATCGT CGCCCAGATA flavo_11_24 CCCTGACTTAACAAACA alpha_4_24GCGTCACCGACAGG gamma_19_24 GCAGGCTCATCTGAT GCCTGCGG CATGCCTGCCAAGCGAAACCT flavo_11_25 ACCGTTGAGCGGTAGG alpha_4_25 TACTCACCCGTCTGCgamma_19_25 CGACGCCTAAGAGCA ATTTCACCC CACTATATCG AGCTCTTATC flavo_12_1CGTCTTCCTGCACGCTG wolbach_1_1 GCCAGGACTTCTTCT gamma_20_1 CCACTAAGGGACAAACATGGCTG GTGAGTACCG TTCCCCCAAC flavo_12_2 CCGTCTTCCTGCACGCT wolbach_1_2AGCCAGGACTTCTT gamma_20_2 CGCCACTAAGGGACA GCATGGCT CTGTGAGTACCAATTCCCCCA flavo_12_3 GTCTTCCTGCACGCTGC wolbach_1_3 CCAGGACTTCTTCTGgamma_20_3 GCCACTAAGGGACAA ATGGCTGG TGAGTACCGT ATTCCCCCAA flavo_12_4CTTCCTGCACGCTGCAT wolbach_1_4 CGGAGTTAGCCAGG gamma_20_4 CACTAAGGGACAAATGGCTGGAT ACTTCTTCTGT TCCCCCAACG flavo_12_5 TTCCTGCACGCTGCATG wolbach_1_5CCGGCCGAACCGAC gamma_20_5 ACTAAGGGACAAATT GCTGGATC CCTATCCCTTCCCCCCAACGG flavo_12_6 GCCGTCTTCCTGCACGC wolbach_1_6 ACGGAGTTAGCCAGgamma_20_6 CTAAGGGACAAATTC TGCATGGC GACTTCTTCTG CCCCAACGGC flavo_12_7TCTTCCTGCACGCTGCA wolbach_1_7 GGAGTTAGCCAGGA gamma_20_7 GCGCCACTAAGGGACTGGCTGGA CTTCTTCTGTG AAATTCCCCC flavo_12_8 CACGCTGCATGGCTGGA wolbach_1_8CAGGACTTCTTCTGT gamma_20_8 GGTACCGTCAAGACG TCAGAGTT GAGTACCGTCCGCAGTTATT flavo_12_9 GGCCGTCTTCCTGCACG wolbach_1_9 GGCACGGAGTTAGCgamma_20_9 AGGTACCGTCAAGAC CTGCATGG CAGGACTTCTT GCGCAGTTAT flavo_12_10TGCCCACCTTTTACCAC wolbach_1_10 CACGGAGTTAGCCA gamma_20_10TAGGTACCGTCAAGA CGGAGTTT GGACTTCTTCT CGCGCAGTTA flavo_12_11ATGCCCACCTTTTACCA wolbach_1_11 TGGCACGGAGTTAG gamma_20_11TGCGCCACTAAGGGA CCGGAGTT CCAGGACTTCT CAAATTCCCC flavo_12_12CACACGTGGACAGATTT wolbach_1_12 GCACGGAGTTAGCC gamma_20_12TAAGGGACAAATTCC CTTCCTGT AGGACTTCTTC CCCAACGGCT flavo_12_13GAAGACTCGCTCTTCCT wolbach_1_13 CGCCTCAGCGTCAG gamma_20_13CTGTAGGTACCGTCA CGCGGAGT ATTTGAACCAG AGACGCGCAG flavo_12_14CATGCCCACCTTTTACC wolbach_1_14 GCGCCTCAGCGTCA gamma_20_14GTAGGTACCGTCAAG ACCGGAGT GATTTGAACCA ACGCGCAGTT flavo_12_15CCGGCTTTGAAGACTCG wolbach_1_15 CTGGCACGGAGTTA gamma_20_15CTGCGCCACTAAGGG CTCTTCCT GCCAGGACTTC ACAAATTCCC flavo_12_16CCACACGTGGACAGATT wolbach_1_16 CTGCTGGCACGGAG gamma_20_16TGTAGGTACCGTCAA TCTTCCTG TTAGCCAGGAC GACGCGCAGT flavo_12_17TTTGAAGACTCGCTCTT wolbach_1_17 GCTGGCACGGAGTT gamma_20_17TCTGTAGGTACCGTC CCTCGCGG AGCCAGGACTT AAGACGCGCA flavo_12_18GGCTTTGAAGACTCGCT wolbach_1_18 TGCTGGCACGGAGT gamma_20_18GCTGCGCCACTAAGG CTTCCTCG TAGCCAGGACT GACAAATTCC flavo_12_19CTTTGAAGACTCGCTCT wolbach_1_19 CGCGCCTCAGCGTC gamma_20_19CTTCTGTAGGTACCGT TCCTCGCG AGATTTGAACC CAAGACGCG flavo_12_20TGAAGACTCGCTCTTCC wolbach_1_20 GCCTTCGCGCCTCA gamma_20_20TCTTCTGTAGGTACCG TCGCGGAG GCGTCAGATTT TCAAGACGC flavo_12_21GACCGGCTTTGAAGACT wolbach_1_21 GCCTCAGCGTCAGA gamma_20_21GGACAAATTCCCCCA CGCTCTTC TTTGAACCAGA ACGGCTAGTT flavo_12_22CGGCTTTGAAGACTCGC wolbach_1_22 TCGCGCCTCAGCGT gamma_20_22GACAAATTCCCCCAA TCTTCCTC CAGATTTGAAC CGGCTAGTTG flavo_12_23GCTTTGAAGACTCGCTC wolbach_1_23 CATGCAACACCTGT gamma_20_23AGCTGCGCCACTAAG TTCCTCGC GTGAAACCCGG GGACAAATTC flavo_12_24ACCGGCTTTGAAGACTC wolbach_1_24 GACTTTGCAGCCCA gamma_20_24CGTTACGCACCCGTC GCTCTTCC TTGTAGCCACC CGCCACTCGA flavo_12_25TCGTACAGTACCGTCAA wolbach_1_25 CGACTTTGCAGCCC gamma_20_25TCGCGTTAGCTGCGC CTACCCAC ATTGTAGCCAC CACTAAGGGA flavo_13_1CGCCGGTCGTCAGCATA rickett_1_1 TCTCTGCGATCCGCG gamma_21_1 TCGTCAGCGCAGAGCGCAAGCTA ACCACCATGT AAGCTCCGCC flavo_13_2 AGGTCGCTCCTCACGGT rickett_1_2ATCTCTGCGATCCGC gamma_21_2 CTCGTCAGCGCAGAG AACGAACT GACCACCATGCAAGCTCCGC flavo_13_3 GGTCGCTCCTCACGGTA rickett_1_3 GTCAGTTGTAGCCCgamma_21_3 ACTCGTCAGCGCAGA ACGAACTT AGATGACCGCC GCAAGCTCCG flavo_13_4TAGGTCGCTCCTCACGG rickett_1_4 CAGTTGTAGCCCAG gamma_21_4 AGCAAGCTCCGCCTGTAACGAAC ATGACCGCCTT TTACCGTTCG flavo_13_5 AGGACGCATAGTCATCT rickett_1_5TCAGTTGTAGCCCA gamma_21_5 GTCAGCGCAGAGCAA TGTACCCA GATGACCGCCTGCTCCGCCTG flavo_13_6 CCTCACGGTAACGAACT rickett_1_6 CGTCAGTTGTAGCCgamma_21_6 GAGCAAGCTCCGCCT TCAGGCAC CAGATGACCGC GTTACCGTTC flavo_13_7TCGCCCAGTGGCTGCTC rickett_1_7 GTTGTAGCCCAGAT gamma_21_7 CAAGCTCCGCCTGTTATTGTCCA GACCGCCTTCG ACCGTTCGAC flavo_13_8 CGTTCGCCGGTCGTCAG rickett_1_8AGTTGTAGCCCAGA gamma_21_8 GCTCCGCCTGTTACCG CATAGCAA TGACCGCCTTCTTCGACTTG flavo_13_9 GTCGCTCCTCACGGTAA rickett_1_9 CATCTCTGCGATCCGgamma_21_9 CTGGGCTTTCACATCC CGAACTTC CGACCACCAT GACTGACCG flavo_13_10GTCGCCCAGTGGCTGCT rickett_1_10 GCGTCAGTTGTAGC gamma_21_10CTTTTGCAAGCCACTC CATTGTCC CCAGATGACCG CCATGGTGT flavo_13_11TAGGACGCATAGTCATC rickett_1_11 AGCATCTCTGCGAT gamma_21_11TCTTTTGCAAGCCACT TTGTACCC CCGCGACCACC CCCATGGTG flavo_13_12ACCAGTATCAAAGGCA rickett_1_12 GCATCTCTGCGATCC gamma_21_12CTTCTTTTGCAAGCCA GTTCCATCG GCGACCACCA CTCCCATGG flavo_13_13TCCTCACGGTAACGAAC rickett_1_13 TTGTAGCCCAGATG gamma_21_13TTTTGCAAGCCACTCC TTCAGGCA ACCGCCTTCGC CATGGTGTG flavo_13_14CTAGGTCGCTCCTCACG rickett_1_14 AGCGTCAGTTGTAG gamma_21_14TTTGCAAGCCACTCCC GTAACGAA CCCAGATGACC ATGGTGTGA flavo_13_15CTCCTCACGGTAACGAA rickett_1_15 CCACTAACTAATTG gamma_21_15CCTCAGCGTCAGTATT CTTCAGGC GAGCAAGCCCC GCTCCAGAA flavo_13_16CCGTTCGCCGGTCGTCA rickett_1_16 GCCACTAACTAATT gamma_21_16GGGCTTTCACATCCG GCATAGCA GGAGCAAGCCC ACTGACCGTG flavo_13_17GTTCGCCGGTCGTCAGC rickett_1_17 CAAGCCCCAATTAG gamma_21_17CTTTCACATCCGACTG ATAGCAAG TCCGTTCGACT ACCGTGCCG flavo_13_18CTCACGGTAACGAACTT rickett_1_18 CCGTCTTGCTTCCCT gamma_21_18GGCTTTCACATCCGA CAGGCACT CTGTAAACAC CTGACCGTGC flavo_13_19TCGCTCCTCACGGTAAC rickett_1_19 CCGTCTGCCACTAA gamma_21_19CACTCGTCAGCGCAG GAACTTCA CTAATTGGAGC AGCAAGCTCC flavo_13_20GGTCGCCCAGTGGCTGC rickett_1_20 CTCTGCGATCCGCG gamma_21_20GCTTTCACATCCGACT TCATTGTC ACCACCATGTC GACCGTGCC flavo_13_21CGGCATAGCTGGTTCAG rickett_1_21 GCAAGCCCCAATTA gamma_21_21TCAGCGCAGAGCAAG AGTTGCCT GTCCGTTCGAC CTCCGCCTGT flavo_13_22GGCATAGCTGGTTCAGA rickett_1_22 AGCAAGCCCCAATT gamma_21_22CGTCAGCGCAGAGCA GTTGCCTC AGTCCGTTCGA AGCTCCGCCT flavo_13_23CGCGGCATAGCTGGTTC rickett_1_23 TGTAGCCCAGATGA gamma_21_23AGAGCAAGCTCCGCC AGAGTTGC CCGCCTTCGCC TGTTACCGTT flavo_13_24GCGGCATAGCTGGTTCA rickett_1_24 GAGCAAGCCCCAAT gamma_21_24AGCTCCGCCTGTTACC GAGTTGCC TAGTCCGTTCG GTTCGACTT flavo_13_25GCATAGCTGGTTCAGAG rickett_1_25 GAAGAAAAGCATCT gamma_21_25CAGAGCAAGCTCCGC TTGCCTCC CTGCGATCCGC CTGTTACCGT flavo_14_1GTGCAAGCACTCCTGTT alpha_5_1 ACCAAAGCCCTGTG verru_1_1 CCCCGAGATTTCACAACCCCTCG GGCCCTAGCAG CCTCACACAT flavo_14_2 AGTGCAAGCACTCCTGT alpha_5_2CACCAAAGCCCTGT verru_1_2 CCCGAGATTTCACAC TACCCCTC GGGCCCTAGCA CTCACACATCflavo_14_3 GCAAGCACTCCTGTTAC alpha_5_3 CCAAAGCCCTGTGG verru_1_3TCACACCTCACACAT CCCTCGAC GCCCTAGCAGC CTATCCGCCT flavo_14_4TGCAAGCACTCCTGTTA alpha_5_4 ACCCTATGGTAGAT verru_1_4 CACCTCACACATCTATCCCCTCGA CCCCACGCGTT CCGCCTACG flavo_14_5 CAAGCACTCCTGTTACC alpha_5_5CACCCTATGGTAGA verru_1_5 TTCACACCTCACACAT CCTCGACT TCCCCACGCGT CTATCCGCCflavo_14_6 AAGCACTCCTGTTACCC alpha_5_6 GCACCCTATGGTAG verru_1_6ACACCTCACACATCT CTCGACTT ATCCCCACGCG ATCCGCCTAC flavo_14_7AGCACTCCTGTTACCCC alpha_5_7 CCGCACCCTATGGT verru_1_7 CACACCTCACACATCTCGACTTG AGATCCCCACG TATCCGCCTA flavo_14_8 GCACTCCTGTTACCCCT alpha_5_8CGCACCCTATGGTA verru_1_8 GCCCCGAGATTTCAC CGACTTGC GATCCCCACGC ACCTCACACAflavo_14_9 TGCTACACGTAGCAGTG alpha_5_9 TATTCCGCACCCTAT verru_1_9ACCTCACACATCTATC TTTCTTCC GGTAGATCCC CGCCTACGC flavo_14_10CCCGTGCGCCGGTCGTC alpha_5_10 ATTCCGCACCCTATG verru_1_10 AGCCCCGAGATTTCAAGCGAGTG GTAGATCCCC CACCTCACAC flavo_14_11 TCGTCAGCGAGTGCAAG alpha_5_11TCCGCACCCTATGGT verru_1_11 CTCCCGAAGGATAGC CACTCCTG AGATCCCCACTCACGTACTT flavo_14_12 TGCGCCGGTCGTCAGCG alpha_5_12 CGCACCAGCTTCGGverru_1_12 CTGCCTCCCGAAGGA AGTGCAAG GTTGATCCAAC TAGCTCACGT flavo_14_13CGGTCGTCAGCGAGTGC alpha_5_13 TTCCGCACCCTATGG verru_1_13 GGCTATGAACCTCCTTAAGCACTC TAGATCCCCA GTTGCTCCT flavo_14_14 CCGTGCGCCGGTCGTCA alpha_5_14CCACCAAAGCCCTG verru_1_14 CCTCCCGAAGGATAG GCGAGTGC TGGGCCCTAGCCTCACGTACT flavo_14_15 GCGCCGGTCGTCAGCGA alpha_5_15 CCCTATGGTAGATCverru_1_15 CCCGAAGGATAGCTC GTGCAAGC CCCACGCGTTA ACGTACTTCG flavo_14_16GGTCGTCAGCGAGTGCA alpha_5_16 CCTATGGTAGATCC verru_1_16 TCCCGAAGGATAGCTAGCACTCC CCACGCGTTAC CACGTACTTC flavo_14_17 GCCGGTCGTCAGCGAGT alpha_5_17GCGCACCAGCTTCG verru_1_17 GAGGCTATGAACCTC GCAAGCAC GGTTGATCCAACTTGTTGCTC flavo_14_18 GTCAGCGAGTGCAAGC alpha_5_18 GCACCAGCTTCGGGverru_1_18 GACGCTGCCTCCCGA ACTCCTGTT TTGATCCAACT AGGATAGCTC flavo_14_19CCGGTCGTCAGCGAGTG alpha_5_19 AGCGCACCAGCTTC verru_1_19 AGGCTATGAACCTCCCAAGCACT GGGTTGATCCA TTGTTGCTCC flavo_14_20 TCAGCGAGTGCAAGCA alpha_5_20CTATGGTAGATCCC verru_1_20 GCCTCCCGAAGGATA CTCCTGTTA CACGCGTTACGGCTCACGTAC flavo_14_21 CGTGCGCCGGTCGTCAG alpha_5_21 GCCACCAAAGCCCTverru_1_21 CGCTGCCTCCCGAAG CGAGTGCA GTGGGCCCTAG GATAGCTCAC flavo_14_22CGCCGGTCGTCAGCGAG alpha_5_22 CACCAGCTTCGGGT verru_1_22 TGCCTCCCGAAGGATTGCAAGCA TGATCCAACTC AGCTCACGTA flavo_14_23 GTGCGCCGGTCGTCAGC alpha_5_23TAGCGCACCAGCTT verru_1_23 ACGCTGCCTCCCGAA GAGTGCAA CGGGTTGATCCGGATAGCTCA flavo_14_24 CGTCAGCGAGTGCAAG alpha_5_24 CAAAGCCCTGTGGGverru_1_24 GCTGCCTCCCGAAGG CACTCCTGT CCCTAGCAGCT ATAGCTCACG flavo_14_25GTCGTCAGCGAGTGCAA alpha_5_25 CGCCACCAAAGCCC verru_1_25 AGGACGCTGCCTCCCGCACTCCT TGTGGGCCCTA GAAGGATAGC flavo_15_1 GGCGTACTCCCCAGGTG alpha_6_1GCGCCACTAACCCC verru_2_1 CGTCGCATGTTCACA CATCACTT GAAGCTTCGTT CTTTCGTGCAflavo_15_2 CTCCCCAGGTGCATCAC alpha_6_2 CTTCTTGCGAGTAGC verru_2_2CTACCCTAACTTTCGT TTAATACT TGCCCACTGT CCATGAGCG flavo_15_3GCGTACTCCCCAGGTGC alpha_6_3 CCCAGCTTGTTGGG verru_2_3 ACCCTAACTTTCGTCCATCACTTA CCATGAGGACT ATGAGCGTC flavo_15_4 CGGCGTACTCCCCAGGT alpha_6_4ATCTTCTTGCGAGTA verru_2_4 GCGTCGCATGTTCAC GCATCACT GCTGCCCACT ACTTTCGTGCflavo_15_5 ACTCCCCAGGTGCATCA alpha_6_5 TCTTCTTGCGAGTAG verru_2_5CAAGTGTTCCCTTCTC CTTAATAC CTGCCCACTG CCCTCCAGT flavo_15_6CGTACTCCCCAGGTGCA alpha_6_6 TAGCCCAGCTTGTTG verru_2_6 TACACCAAGTGTTCCTCACTTAA GGCCATGAGG CTTCTCCCCT flavo_15_7 CCGGCGTACTCCCCAGG alpha_6_7GCCACTAACCCCGA verru_2_7 CCAAGTGTTCCCTTCT TGCATCAC AGCTTCGTTCG CCCCTCCAGflavo_15_8 GTACTCCCCAGGTGCAT alpha_6_8 GTAGCCCAGCTTGTT verru_2_8ACACCAAGTGTTCCC CACTTAAT GGGCCATGAG TTCTCCCCTC flavo_15_9GCCGGCGTACTCCCCAG alpha_6_9 CGCCACTAACCCCG verru_2_9 CGCTACACCAAGTGTGTGCATCA AAGCTTCGTTC TCCCTTCTCC flavo_15_10 GAAGAGAAGGCCTGTTT alpha_6_10TTCTTGCGAGTAGCT verru_2_10 CACCAAGTGTTCCCTT CCAAGCCG GCCCACTGTCCTCCCCTCC flavo_15_11 CAACAGCGAGTGATGA alpha_6_11 TAGCATCTTCTTGCGverru_2_11 GCTACACCAAGTGTT TCGTTTACG AGTAGCTGCC CCCTTCTCCC flavo_15_12GCATGCCCATCTCATAC alpha_6_12 AGCATCTTCTTGCGA verru_2_12 CTACACCAAGTGTTCCGAAAAAC GTAGCTGCCC CCTTCTCCCC flavo_15_13 TTGTAATCTGCTCCGAA alpha_6_13GCCCAGCTTGTTGG verru_2_13 AGTGTTCCCTTCTCCC GAGAAGGC GCCATGAGGACCTCCAGTAC flavo_15_14 CGCCGGTCGTCAGCAAA alpha_6_14 CACTAACCCCGAAGverru_2_14 AAGTGTTCCCTTCTCC AGCAAGCT CTTCGTTCGAC CCTCCAGTA flavo_15_15AAGAGAAGGCCTGTTTC alpha_6_15 CATCTTCTTGCGAGT verru_2_15 ACCAAGTGTTCCCTTCCAAGCCGG AGCTGCCCAC TCCCCTCCA flavo_15_16 GCCGGTCGTCAGCAAA alpha_6_16TGTAGCCCAGCTTGT verru_2_16 GCTACCCTAACTTTCG AGCAAGCTT TGGGCCATGATCCATGAGC flavo_15_17 TGCCGGCGTACTCCCCA alpha_6_17 AGCCCAGCTTGTTGverru_2_17 GTTCCCTTCTCCCCTC GGTGCATC GGCCATGAGGA CAGTACTCT flavo_15_18GCGCCGGTCGTCAGCAA alpha_6_18 CCACTAACCCCGAA verru_2_18 GTGTTCCCTTCTCCCCAAGCAAGC GCTTCGTTCGA TCCAGTACT flavo_15_19 CGAAGAGAAGGCCTGT alpha_6_19GCATCTTCTTGCGAG verru_2_19 TGTTCCCTTCTCCCCT TTCCAAGCC TAGCTGCCCACCAGTACTC flavo_15_20 CCAACAGCGAGTGATG alpha_6_20 GTGTAGCCCAGCTTverru_2_20 CCGCTACACCAAGTG ATCGTTTAC GTTGGGCCATG TTCCCTTCTC flavo_15_21GGAGTATTAATCCCCGT alpha_6_21 TGCGCCACTAACCC verru_2_21 TTCCCTTCTCCCCTCCTTCCAGGG CGAAGCTTCGT AGTACTCTA flavo_15_22 TGGAGTATTAATCCCCG alpha_6_22CTCAAGCACCAAGT verru_2_22 GGCGTCGCATGTTCA TTTCCAGG GCCCGAACAGCCACTTTCGTG flavo_15_23 TCCCCGTTTCCAGGGGC alpha_6_23 CCAGCTTGTTGGGCverru_2_23 CGCTACCCTAACTTTC TATCCTCC CATGAGGACTT GTCCATGAG flavo_15_24TGCGCCGGTCGTCAGCA alpha_6_24 ACTAACCCCGAAGC verru_2_24 CCCTAACTTTCGTCCAAAAGCAAG TTCGTTCGACT TGAGCGTCA flavo_15_25 AACAGCGAGTGATGAT alpha_6_25TCTTGCGAGTAGCTG verru_2_25 ACCGCTACACCAAGT CGTTTACGG CCCACTGTCAGTTCCCTTCT

The Chip-SIP method was applied to San Francisco Bay water collected atthe Berkeley Calif. pier, incubated in the presence of 200 uM ¹⁵Nammonium for 24 hours and sampled over this time. An array designed totarget marine microorganisms was designed using ARB software; where eachrow on the array represents a series of probes designed to hybridize toa different taxon (microbial species).

A collected environmental water sample was analyzed by Chip-SIP. Inparticular San Francisco Bay water was collected at the Berkeley pier,and incubated with 200 uM ¹⁵N ammonium for 24 hours. An array designedto target marine microorganisms was designed using built with ARBsoftware.

To construct the network diagram of FIG. 10B, taxa with HCEs havingstandard errors not overlapping with zero and with >30 permil enrichmentwere included (all others were discarded) using Cytoscape software (17).For analyses of marine bacterial genomic information, genomes of marinebacterial isolates were selected in the Joint Genome Institute'sIntegrated Microbial Genomes (IM-G) database and word-searched for thepresence of amino acid, fatty acid, and nucleoside transporters andextracellular nucleases. Results are summarized in Table 2.

TABLE 2 Amino acid Extracellular Nucleoside Fatty acid Genome transportnuclease transport transport Agreia sp. PHSC20C1 Y N N N Algoriphagussp. PR1 Y N Y Y Aurantimonas sp. SI85-9A1 Y N N N Bacillus sp. B14905 YN Y N Bacillus sp. NRRL B-14911 Y N Y N Bacillus sp. SG-1 Y Y Y NBeggiatoa sp. PS Y N N Y Bermanella marisrubri Y N N Y Blastopirellulamarina DSM 3645 Y Y N N Caminibacter mediatlanticus TB-2 Y N N NCandidatus Blochmannia Y N N N pennsylvanicus BPEN CandidatusPelagibacter ubique Y N N N HTCC1002 Carnobacterium sp. AT7 Y N Y YCongregibacter litoralis KT71 Y N Y N Croceibacter atlanticus HTCC2559 YY Y N Cyanothece sp. CCY 0110 Y N Y N Dokdonia donghaensis MED134 Y Y YN Erythrobacter litoralis HTCC2594 Y N Y Y Erythrobacter sp. NAP1 Y N NN Erythrobacter sp. SD-21 Y N Y N Finegoldia magna ATCC 29328 Y N N NFlavobacteria bacterium BAL38 Y N N Y Flavobacteria bacterium BBFL7 N YN N Flavobacteriales bacterium ALC-1 Y N Y N Flavobacteriales bacteriumY N Y N HTCC2170 Fulvimarina pelagi HTCC2506 Y N N Y Hoefleaphototrophica DFL-43 Y N N Y Hydrogenivirga sp. 128-5-R1-1 Y N N NIdiomarina baltica OS145 Y Y N Y Janibacter sp. HTCC2649 Y Y N N Kordiaalgicida OT-1 Y Y N Y Labrenzia aggregata IAM 12614 Y N N NLeeuwenhoekiella blandensis Y N Y N MED217 Lentisphaera araneosaHTCC2155 Y N N Y Limnobacter sp. MED105 Y N N Y Loktanella vestfoldensisSKA53 Y N N N Lyngbya sp. PCC 8106 Y Y Y N marine gamma proteobacteriumY Y Y Y HTCC2080 marine gamma proteobacterium Y N N N HTCC2143 marinegamma proteobacterium Y N N N HTCC2148 marine gamma proteobacterium Y NN N HTCC2207 Marinobacter algicola DG893 Y Y N Y Marinobacter sp. ELB17Y N N Y Marinomonas sp. MED121 Y Y N N Mariprofundus ferrooxydans PV-1 YN N Y Methylophilales bacterium N N N N HTCC2181 Microscilla marina ATCC23134 Y Y Y N Moritella sp. PE36 Y Y Y Y Neptuniibacter caesariensis Y NN N Nisaea sp. BAL199 Y N N Y Nitrobacter sp. Nb-311A Y N N NNitrococcus mobilis Nb-231 Y N N N Nodularia spumigena CCY9414 Y N N NOceanibulbus indolifex HEL-45 Y N Y Y Oceanicaulis alexandrii HTCC2633 YN N N Oceanicola batsensis HTCC2597 Y Y N N Oceanicola granulosusHTCC2516 Y Y Y N Parvularcula bermudensis Y Y Y N HTCC2503 Pedobactersp. BAL39 Y N N Y Pelotomaculum thermopropionicum Y N N N SI Phaeobactergallaeciensis 2.10 Y N N N Phaeobacter gallaeciensis BS107 Y N N NPhotobacterium angustum S14 Y Y Y Y Photobacterium profundum 3TCK Y Y YY Photobacterium sp. SKA34 Y Y Y Y Planctomyces maris DSM 8797 Y Y N NPlesiocystis pacifica SIR-1 Y N Y Y Polaribacter irgensii 23-P Y Y Y NPolaribacter sp. MED152 Y Y Y N Prochlorococcus marinus AS9601 N N N NProchlorococcus marinus MIT 9211 Y N N N Prochlorococcus marinus MIT9301 N N N N Prochlorococcus marinus MIT 9303 Y N N N Prochlorococcusmarinus MIT 9515 Y N N N Prochlorococcus marinus NATL1A Y N N NPseudoalteromonas sp. TW-7 Y N Y Y Pseudoalteromonas tunicata D2 Y N Y YPsychroflexus torquis ATCC Y Y N N 700755 Psychromonas sp. CNPT3 Y N N YReinekea sp. MED297 Y Y N N Rhodobacterales bacterium Y Y Y N HTCC2150Rhodobacterales bacterium Y N N N HTCC2654 Rhodobacterales sp. HTCC2255Y N Y N Roseobacter litoralis Och 149 Y N N N Roseobacter sp. AzwK-3b YN N N Roseobacter sp. CCS2 Y Y N N Roseobacter sp. MED193 Y N N NRoseobacter sp. SK209-2-6 Y N Y N Roseovarius nubinhibens ISM Y N N NRoseovarius sp. 217 Y Y Y Y Roseovarius sp. HTCC2601 Y N Y N Roseovariussp. TM1035 Y N N N Sagittula stellata E-37 Y Y N N Shewanella benthicaKT99 Y Y N Y Sphingomonas sp. SKA58 Y N Y Y Sulfitobacter sp. EE-36 Y NN N Sulfitobacter sp. NAS-14.1 Y N N N Synechococcus sp. BL107 Y N N NSynechococcus sp. RS9916 Y N N N Synechococcus sp. RS9917 Y N N NSynechococcus sp. WH 5701 Y N N N Synechococcus sp. WH 7805 Y N N NUlvibacter sp. SCB49 Y Y N Y Vibrio alginolyticus 12G01 Y Y Y Y Vibriocampbellii AND4 Y N Y Y Vibrio harveyi HY01 Y N Y Y Vibrio shilonii AK1Y Y Y Y Vibrio sp. MED222 Y Y Y Y Vibrio splendidus 12B01 Y Y Y YVibrionales bacterium SWAT-3 Y Y Y Y

For phylogenetic relationships the global 16S rRNA phylogeny in theGreengenes database (18) was opened in ARB (19) and all taxa except thetargets of the array analysis were removed with the taxon pruningfunction.

As evident in the hybridization patterns measured (FIG. 10A) and inNanoSIMS enrichment data measured on this ITO array, different taxaincorporated ammonia at different rates during the experiment. Theseexperiments show that different marine microbial taxa are present atdifferent time points, and that different taxa incorporated ammonia atdifferent times and to differing degrees. This set of data furtherdemonstrates that the Chip-SIP method can be used to characterizedcomplex mixtures of nucleic acids

In a similar experiment, where isotopically labeled nucleic acids, aminoacids, and fatty acids were added as microbial substrates, Chip-SIP wasable to identify substrate specialist and generalist taxa (FIG. 10B),based on the organisms that took up all three substrates into their RNA(generalists), versus those that only took up one of the possiblesubstrates (specialists).

The results illustrated in FIG. 10B show that different marine microbialtaxa have different substrate use patterns in an environmental watersample analyzed by Chip-SIP. This demonstrates that the Chip-SIP methodcan be used to quantitatively characterized the substrate uptakepatterns of complex microbial communities.

Example 7 Chip-SIP and Related Manufacture and Use

A functionalized microarray was manufactured comprising a definedplurality of single-strand DNA molecules that have been chemicallysynthesized on the surface of a standard glass microscope slide.Importantly, the latter has been coated with a conductive layerconsisting of inorganic indium-tin-oxide (16) between 300 and 1500angstroms in thickness—such glass microscope slides are commerciallyavailable from Sigma Chemical Company, St. Louis, Mo. The ITO surface istreated with a linker molecule to provide a starting point for DNAsynthesis.

Such linker molecules contain a chemical group that reacts specificallywith the ITO surface, e.g. silanes, phosphonates and the like; as wellas a chemical group that provides a starting point for DNA synthesis,e.g. hydroxyl (—OH), amino (—NH₂) and the like. These functionalizedglass microscope slides are placed in a Maskless Array Synthesizer (MAS)unit; the MAS is programmed to synthesize a plurality of uniquesingle-strand DNA molecules each within a feature size between 13-15micron². Subsequent hybridization with complementary single strandoligonucleotides containing stable isotopes, e.g. C¹³ and N¹⁵, resultsin double-stranded molecular assemblies labeled with stable isotopes.The latter can be detected and quantified by secondary mass spectrometryor SIMS as described herein.

In summary, in several embodiments, polymer arrays are described thatare suitable to perform quantitative and qualitative detection as wellas sorting of a target molecules and related devices methods andsystems.

The examples set forth above are provided to give those of ordinaryskill in the art a complete disclosure and description of how to makeand use the embodiments of the compositions, systems and methods of thedisclosure, and are not intended to limit the scope of what theinventors regard as their disclosure. All patents and publicationsmentioned in the specification are indicative of the levels of skill ofthose skilled in the art to which the disclosure pertains.

The entire disclosure of each document cited (including patents, patentapplications, journal articles, abstracts, laboratory manuals, books, orother disclosures) in the Background, Summary, Detailed Description, andExamples is hereby incorporated herein by reference. All referencescited in this disclosure are incorporated by reference to the sameextent as if each reference had been incorporated by reference in itsentirety individually.

Further, the sequence listing annexed herewith in computer readable formforms integral part of this description and is incorporated herein byreference in its entirety.

It is to be understood that the disclosures are not limited toparticular compositions or biological systems, which can, of course,vary. It is also to be understood that the terminology used herein isfor the purpose of describing particular embodiments only, and is notintended to be limiting. As used in this specification and the appendedclaims, the singular forms “a,” “an,” and “the” include plural referentsunless the content clearly dictates otherwise. The term “plurality”includes two or more referents unless the content clearly dictatesotherwise. Unless defined otherwise, all technical and scientific termsused herein have the same meaning as commonly understood by one ofordinary skill in the art to which the disclosure pertains.

When a Markush group or other grouping is used herein, all individualmembers of the group and all combinations and possible subcombinationsof the group are intended to be individually included in the disclosure.Every combination of components or materials described or exemplifiedherein can be used to practice the disclosure, unless otherwise stated.One of ordinary skill in the art will appreciate that methods, deviceelements, and materials other than those specifically exemplified can beemployed in the practice of the disclosure without resort to undueexperimentation. All art-known functional equivalents, of any suchmethods, device elements, and materials are intended to be included inthis disclosure. Whenever a range is given in the specification, forexample, a temperature range, a frequency range, a time range, or acomposition range, all intermediate ranges and all subranges, as wellas, all individual values included in the ranges given are intended tobe included in the disclosure. Any one or more individual members of arange or group disclosed herein can be excluded from a claim of thisdisclosure. The disclosure illustratively described herein suitably canbe practiced in the absence of any element or elements, limitation orlimitations which is not specifically disclosed herein.

Although any methods and materials similar or equivalent to thosedescribed herein can be used in the practice for testing of theproducts, methods and system of the present disclosure, exemplaryappropriate materials and methods are described herein.

A number of embodiments of the disclosure have been described. Thespecific embodiments provided herein are examples of useful embodimentsof the disclosure and it will be apparent to one skilled in the art thatthe disclosure can be carried out using a large number of variations ofthe functionalized platforms, arrays, compositions, methods steps, andsystems set forth in the present description. As will be obvious to oneof skill in the art, methods and devices useful for the present methodscan include a large number of optional composition and processingelements and steps.

It will be understood that various modifications may be made withoutdeparting from the spirit and scope of the present disclosure.Accordingly, other embodiments are within the scope of the followingclaims.

REFERENCES

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1. A method for quantitative detection of a target, the methodcomprising, labeling the target with a SIMS detectable label to providea SIMS labeled target, the SIMS labeled target capable of binding apolymer of a polymer array comprising the polymer presented on aplatform; contacting the SIMS labeled target with the polymer array fora time and under condition to allow binding of the SIMS labeled targetmolecule to the polymer array; and performing SIMS detection of thepolymer array following the contacting to detect the SIM labeled targetbound to the polymer array, wherein the platform comprises a substratecoated with an electrically conductive layer and the polymer is attachedto the platform through a functional linker molecule attached to theelectrically conductive layer.
 2. A method to detect a target in asample, the method comprising: exposing the sample to a label detectableby Secondary Ion Mass Spectrometry (SIMS label) for a time and undercondition to allow binding of the SIMS label with the target; contactingthe polymer array with the sample following the exposing to allowbinding of a SIMS labeled target with a polymer array comprising apolymer presented on a platform; and performing Secondary Ion MassSpectrometry on the polymer array following the contacting to detect theSIM labeled target bound to the polymer array, wherein the platformcomprises a substrate coated with an electrically conductive layer andthe polymer is attached to the platform through a functional linkermolecule attached to the electrically conductive layer.
 3. The method ofclaim 1 or 2, wherein the SIMS label is a stable isotope.
 4. The methodof claim 1 or 2, wherein the polymer is a probe nucleic acid, the targetis a target nucleic acids and the contacting is performed to allowspecific hybridization of the probe nucleic acid with the target nucleicacid.
 5. A system for detection of a target, the system comprising afunctionalized platform comprising a substrate coated with anelectrically conductive layer attaching a functionalized linkermolecule; and a label detectable by Secondary Ion Mass Spectrometry(SIMS label) wherein the platform is configured to be associated, duringoperation, with a polymer array, the polymer array is configured fordetection of a target attached to a polymer on the polymer array, thedetection performed through the SIMS label attached to the target. 6.The system of claim 5, further comprising the polymer array configuredfor detection of a target attached to a polymer on the polymer arraythrough the SIMS label.
 7. The system of claim 5, wherein theelectrically conductive layer comprises a metal oxide.
 8. The system ofclaim 7, wherein the metal oxide is ITO.
 9. The system of claim 8,wherein ITO comprises about 90% In₂O₃ and about 10% SnO₂ by weight. 10.The system of claim 9, wherein the substrate is glass, quartz, silica orplastic.
 11. The system of claim 5, wherein the polymer is a nucleicacid.
 12. The system of claim 5, wherein the SIMS label is ¹³C or ¹⁵N.13. The system of claim 5, wherein the polymer array is comprised in abiochip.
 14. A functionalized platform comprising a substrate, and anelectrically conductive layer, wherein the substrate is coated with theelectrically conductive layer and the electrically conductive layerattaches an a functionalized linker molecule comprising an organosilanecompound presenting an organosilane functional group, the platform isconfigured to be associated, during operation, with a polymer arrayconfigured for detection of a target attached to a polymer on thepolymer array, through a label attached to the target, the labeldetectable by Secondary Ion Mass Spectrometry.
 15. A polymer arrayconfigured to allow detection of a target attached to the polymerthrough a label attached to the target, the polymer array comprising apolymer attached to a platform, wherein the platform is thefunctionalized platform of claim 14, the polymer is attached to theorganosilane functional group of the functionalized platform of claim14, and wherein the label is detectable by Secondary Ion MassSpectrometry.
 16. The polymer array of claim 15, wherein the polymer isa polynucleotide or a polypeptide.
 17. The polymer array of claim 15,wherein the polymer is DNA.
 18. The polymer array of claim 15, whereinthe polymer is spotted on the platform.
 19. A bio-chip comprising thepolymer array of claim 15.